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Extraradical mycelium network of arbuscular mycorrhizal fungi allows fast colonization of seedlings under in vitro conditions

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Voets, Liesbeth de la Providencia, Ivan Fernandez, Kalyanne Ijdo, Marleen Cranenbrouck, Sylvie [UCL] Declerck, Stephan [UCL]

Actively growing extraradical hyphae extending from mycorrhizal plants are an important source of inoculum in soils which has seldom been considered in vitro to inoculate young plantlets. Seedlings of Medicago truncatula were grown in vitro in the extraradical mycelium network extending from mycorrhizal plants. After 3, 6, 9, 12, and 15 days of contact with the mycelium, half of the seedlings were harvested and analyzed for root colonization. The other half was carefully transplanted in vitro on a suitable growth medium and mycelium growth and spore production were evaluated for 4 weeks. Seedlings were readily colonized after 3 days of contact with the mycelium. Starting from 6 days of contact, the newly colonized seedlings were able to reproduce the fungal life cycle, with the production of thousands of spores within 4 weeks. The fast mycorrhization process developed here opens the door to a broad range of in vitro studies for which either homogenous highly colonized seedlings or mass-produced in vitro inoculum is necessary.

Document type Article de périodique (Journal article) – Article de recherche – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 2009
Language Anglais
Journal information "Mycorrhiza" - Vol. 19, no. 5, p. 347-56 (2009)
Peer reviewed yes
issn 0940-6360
e-issn 1432-1890
Publication status Publié
Affiliation UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries
MESH Subject Spores, Fungal - growth & development ; Seedling - microbiology ; Plant Roots - growth & development - microbiology ; Mycorrhizae - growth & development ; Mycelium - growth & development ; Medicago truncatula - growth & development - microbiology ; Glomeromycota - growth & development ; Culture Techniques
Keywords Arbuscular mycorrhizal (AM) fungi ; Seedling ; Root organ culture ; Medicago truncatula ; Mycelium network ; 3130
Links
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Bibliographic reference Voets, Liesbeth ; de la Providencia, Ivan ; Fernandez, Kalyanne ; Ijdo, Marleen ; Cranenbrouck, Sylvie ; et. al. Extraradical mycelium network of arbuscular mycorrhizal fungi allows fast colonization of seedlings under in vitro conditions. In: Mycorrhiza, Vol. 19, no. 5, p. 347-56 (2009)
Permanent URL http://hdl.handle.net/2078.1/21481