User menu

Extraradical mycelium network of arbuscular mycorrhizal fungi allows fast colonization of seedlings under in vitro conditions

Bibliographic reference Voets, Liesbeth ; de la Providencia, Ivan ; Fernandez, Kalyanne ; Ijdo, Marleen ; Cranenbrouck, Sylvie ; et. al. Extraradical mycelium network of arbuscular mycorrhizal fungi allows fast colonization of seedlings under in vitro conditions. In: Mycorrhiza, Vol. 19, no. 5, p. 347-56 (2009)
Permanent URL http://hdl.handle.net/2078.1/21481
  1. Bago B, Cano C (2005) Breaking myths on arbuscular mycorrhizas in vitro biology. In: Declerck S, Strullu DG, Fortin JA (eds) In vitro culture of mycorrhizas. Springer, Berlin, pp 111–138
  2. Bago B, Azcon-Aguilar C, Piché Y (1998) Architecture and developmental dynamics of the external mycelium of the arbuscular mycorrhizal fungus Glomus intraradices grown under monoxenic conditions. Mycologia 90:52–62. doi: 10.2307/3761011
  3. Bago B, Pfeffer PE, Zipfel W, Lammers P, Shachar-Hill Y (2002) Tracking metabolism and imaging transport in arbuscular mycorrhizal fungi. Plant Soil 244:189–197. doi: 10.1023/A:1020212328955
  4. Bécard G, Fortin JA (1988) Early events of vesicular arbuscular mycorrhiza formation on Ri-T-DNA transformed roots. New Phytol 108:211–218. doi: 10.1111/j.1469-8137.1988.tb03698.x
  5. Bécard G, Piché Y (1989) Fungal growth stimulation by CO2 and root exudates in vesicular–arbuscular mycorrhizal symbiosis. Appl Environ Microbiol 55:2320–2325
  6. Biermann B, Linderman RG (1983) Use of vesicular arbuscular mycorrhizal roots, intraradical vesicles and extraradical vesicles as inoculum. New Phytol 95:97–105. doi: 10.1111/j.1469-8137.1983.tb03472.x
  7. Cano C, Dickson S, González-Guerrero M, Bago A (2008) In vitro cultures open new prospects for basic research in arbuscular mycorrhizas. In: Varma A (ed) Mycorrhiza. Springer-Verlag, Berlin, pp 627–654
  8. Cranenbrouck S, Voets L, Bivort C, Renard L, Stullu DG, Declerck S (2005) Methodologies for in vitro cultivation of arbuscular mycorrhizal fungi with root organs. In: Declerck S, Strullu DG, Fortin JA (eds) In vitro culture of mycorrhizas. Springer, Berlin, pp 341–348
  9. Dalpé Y, Declerck S (2002) Development of Acaulospora rehmii spore and hyphal swellings under root-organ culture. Mycologia 94:850–855. doi: 10.2307/3761699
  10. de la Providencia IE, de Souza FA, Fernandez F, Delmas NS, Declerck S (2005) Arbuscular mycorrhizal fungi reveal distinct patterns of anastomosis formation and hyphal healing mechanisms between different phylogenic groups. New Phytol 165:261–271. doi: 10.1111/j.1469-8137.2004.01236.x
  11. de la Providencia IE, Fernandez F, Declerck S (2007) Hyphal healing mechanism in the arbuscular mycorrhizal fungi Scutellospora reticulata and Glomus clarum differ in response to severe physical stress. FEMS Microbiol Lett 268:120–125. doi: 10.1111/j.1574-6968.2006.00572.x
  12. de Souza FA, Berbara RLL (1999) Ontogeny of Glomus clarum in Ri T-DNA transformed roots. Mycologia 91:343–350. doi: 10.2307/3761379
  13. de Souza FA, Declerck S (2003) Mycelium development and architecture, and spore production of Scutellospora reticulata in monoxenic culture with Ri T-DNA transformed carrot roots. Mycologia 95:1004–1012. doi: 10.2307/3761908
  14. de Souza FA, Declerck S, Smit E, Kowalchuk GA (2005) Morphological, ontogenetic and molecular characterization of Scutellospora reticulata (Glomeromycota). Mycol Res 109:697–706. doi: 10.1017/S0953756205002546
  15. Declerck S, Strullu DG, Plenchette C (1996) In vitro mass-production of the arbuscular mycorrhizal fungus, Glomus versiforme, associated with Ri T-DNA transformed carrot roots. Mycol Res 100:1237–1242
  16. Declerck S, Strullu DG, Plenchette C (1998) Monoxenic culture of the intraradical forms of Glomus sp. isolated from a tropical ecosystem: a proposed methodology for germplasm collection. Mycologia 90:579–585. doi: 10.2307/3761216
  17. Declerck S, Cranenbrouck S, Dalpé Y, Séguin S, Grandmougin-Ferjani A, Fontaine J, Sancholle M (2000) Glomus proliferum sp nov.: a description based on morphological, biochemical, molecular and monoxenic cultivation data. Mycologia 92:1178–1187. doi: 10.2307/3761485
  18. Declerck S, D'Or D, Cranenbrouck S, Le Boulenge E (2001) Modelling the sporulation dynamics of arbuscular mycorrhizal fungi in monoxenic culture. Mycorrhiza 11:225–230. doi: 10.1007/s005720100124
  19. Declerck S, Strullu DG, Fortin JA (eds) (2005) In vitro culture of mycorrhizas. Springer, Berlin
  20. Diop TA, Plenchette C, Strullu DG (1994a) Dual axenic culture of sheared-root inocula of vesicular–arbuscular mycorrhizal fungi associated with tomato roots. Mycorrhiza 5:17–22. doi: 10.1007/BF00204015
  21. Diop TA, Plenchette C, Strullu DG (1994b) In vitro culture of sheared mycorrhizal roots. Symbiosis 17:217–227
  22. Doner LW, Bécard G (1991) Solubilisation of gellan gels by chelation of cations. Biotechnol Tech 5:25–28. doi: 10.1007/BF00152749
  23. Dupré de Boulois H, Delvaux B, Declerck S (2005) Effects of arbuscular mycorrhizal fungi on the root uptake and translocation of radiocaesium. Environ Pollut 134:515–524. doi: 10.1016/j.envpol.2004.08.015
  24. Dupré de Boulois H, Voets L, Delvaux B, Jakobsen I, Declerck S (2006) Transport of radiocaesium by arbuscular mycorrhizal fungi to Medicago truncatula under in vitro conditions. Environ Microbiol 8:1926–1934. doi: 10.1111/j.1462-2920.2006.01070.x
  25. Elmeskaoui A, Damont J-P, Poulin M-J, Piché Y, Desjardins Y (1995) A tripartite culture system for endomycorrhizal inoculation of micropropagated strawberry plantlets in vitro. Mycorrhiza 5:313–319. doi: 10.1007/BF00207403
  26. Elsen A, Declerck S, De Waele D (2003) Use of root organ cultures to investigate the interaction between Glomus intraradices and Pratylenchus coffeae. Appl Environ Microbiol 69:4308–4311. doi: 10.1128/AEM.69.7.4308-4311.2003
  27. Fortin JA, Bécard G, Declerck S, Dalpé Y, St-Arnaud M, Coughlan AP, Piché Y (2002) Arbuscular mycorrhiza on root-organ cultures. Can J Bot 80:1–20. doi: 10.1139/b01-139
  28. Friese CF, Allen MF (1991) The spread of VA mycorrhizal fungal hyphea in the soil–inoculum types and external hyphal architecture. Mycologia 83:409–418. doi: 10.2307/3760351
  29. Giovannetti M, Sbrana C, Avio L, Strani P (2004) Patterns of below-ground plant interconnections established by means of arbuscular mycorrhizal networks. New Phytol 164:175–181. doi: 10.1111/j.1469-8137.2004.01145.x
  30. Hart MM, Reader RJ (2002) Taxonomic basis for variation in the colonization strategy of arbuscular mycorrhizal fungi. New Phytol 153:335–344. doi: 10.1046/j.0028-646X.2001.00312.x
  31. Hart MM, Reader RJ (2005) The role of the external mycelium in early colonization for three arbuscular mycorhizal fungal species with different colonization strategies. Pedobiologia (Jena) 49:269–279. doi: 10.1016/j.pedobi.2004.12.001
  32. HEPPER CHRISTINE M., TECHNIQUES FOR STUDYING THE INFECTION OF PLANTS BY VESICULAR-ARBUSCULAR MYCORRHIZAL FUNGI UNDER AXENIC CONDITIONS, 10.1111/j.1469-8137.1981.tb01740.x
  33. Hernandez-Sebastia C, Piché Y, Desjardins Y (1999) Water relations of whole strawberry plantlets in vitro inoculated with Glomus intraradices in a tripartite culture system. Plant Sci 143:81–91. doi: 10.1016/S0168-9452(99) 00014-X
  34. Hernandez-Sebastia C, Samson G, Bernier PY, Piché Y, Desjardins Y (2000) Glomus intraradices causes differential changes in amino acid and starch concentrations of in vitro strawberry subjected to water stress. New Phytol 148:177–186. doi: 10.1046/j.1469-8137.2000.00744.x
  35. Klironomos JM, Hart NN (2002) Colonization of roots by arbuscular mycorrhizal fungi using different sources of inoculum. Mycorrhiza 12:181–184. doi: 10.1007/s00572-002-0169-6
  36. Koch AM, Kuhn G, Fontanillas P, Fumagalli L, Goudet J, Sanders IR (2004) High genetic variability and low local diversity in a population of arbuscular mycorrhizal fungi. Proc Natl Acad Sci U S A 101:2369–2374. doi: 10.1073/pnas.0306441101
  37. Louche-Tessandier D, Samson G, Hernandez-Sebastia C, Chagvardieff P, Desjardins Y (1999) Importance of light and CO2 on the effects of endomycorrhizal colonization on growth and photosynthesis of potato plantlets (Solanum tuberosum) in an in vitro tripartite system. New Phytol 142:539–550. doi: 10.1046/j.1469-8137.1999.00408.x
  38. Mosse B, Hepper C (1975) Vesicular arbuscular mycorrhizal infections in root organ cultures. Physiol Plant Pathol 5:215–223. doi: 10.1016/0048-4059(75) 90088-0
  39. Nielsen JS, Joner EJ, Declerck S, Olsson S, Jakobsen I (2002) Phospho-imaging as a tool for visualization and noninvasive measurement of P transport dynamics in arbuscular mycorrhizas. New Phytol 154:809–819. doi: 10.1046/j.1469-8137.2002.00412.x
  40. Pawlowska TE, Taylor JW (2004) Organization of genetic variation in individuals of arbuscular mycorrhizal fungi. Nature 427:733–737. doi: 10.1038/nature02290
  41. Pawlowska TE, Douds DD, Charvat I (1999) In vitro propagation and life cycle of the arbuscular mycorrhizal fungus Glomus etunicatum. Mycol Res 103:1549–1556. doi: 10.1017/S0953756299008801
  42. Read DJ, Francis R, Finlay RD (1985) Mycorrhizal mycelia and nutrient cycling in plant communities. In: Fitter AH (ed) Ecological interactions in soil. Blackwell Scientific, Oxford, pp 193–217
  43. Rufyikiri G, Thiry Y, Wang L, Delvaux B, Declerck S (2002) Uranium uptake and translocation by the arbuscular mycorrhizal fungus Glomus intraradices, under root organ culture conditions. New Phytol 156:275–281. doi: 10.1046/j.1469-8137.2002.00520.x
  44. Rufyikiri G, Thiry Y, Declerck S (2003) Contribution of hyphae and roots to uranium uptake and translocation by arbuscular mycorrhizal carrot roots under root-organ culture conditions. New Phytol 158:391–399. doi: 10.1046/j.1469-8137.2003.00747.x
  45. Smith SE, Read DJ (eds) (1997) Mycorrhizal symbiosis, 2nd edn. Academic, San Diego
  46. St-Arnaud M, Hamel C, Vimard B, Caron M, Fortin JA (1996) Enhanced hyphal growth and spore production of the arbuscular mycorrhizal fungus Glomus intraradices in an in vitro system in the absence of host roots. Mycol Res 100:328–332
  47. StatSoft Inc (2001) Statistica® release 6. Statsoft Incorporation, Tulsa
  48. Strullu DG, Romand C (1986) Méthode d’obtention d’endomycorhizes à vésicules et arbuscules en conditions axéniques. C R Acad Sci 303:245–250
  49. Vierheilig H, Coughlan AP, Wyss U, Piché Y (1998) Ink and vinegar, a simple staining technique for arbuscular mycorrhizal fungi. Appl Environ Microbiol 64:5004–5007
  50. Voets L, Dupré de Boulois H, Renard L, Strullu DG, Declerck S (2005) Development of an autotrophic culture system for the in vitro mycorrhization of potato plantlets. FEMS Microbiol Lett 248:111–118. doi: 10.1016/j.femsle.2005.05.025
  51. Voets L, de la Providencia IE, Declerck S (2006) Glomeraceae and Gigasporaceae differ in their ability to form mycelium networks. New Phytol 172:185–188. doi: 10.1111/j.1469-8137.2006.01873.x
  52. Voets L, Goubau I, Olsson PA, Merckx R, Declerck S (2008) Absence of carbon transfer between Medicago truncatula plants linked by a mycorrhizal network, demonstrated in an experimental microcosm. FEMS Microbiol Ecol 65:350–360. doi: 10.1111/j.1574-6941.2008.00503.x