User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

Butyricimonas faecalis sp. nov., isolated from human faeces and emended description of the genus Butyricimonas.

Primary tabs

download
  • Open access
  • PDF
  • 200.93 K
Le Roy, Tiphaine [UCL] Van Der Smissen, Patrick [UCL] Paquot, Adrien [UCL] Delzenne, Nathalie M. [UCL] Muccioli, Giulio [UCL] Collet, Jean-François [UCL] Cani, Patrice D. [UCL]

A Gram-negative, strictly anaerobic, non-spore forming, non-motile, non-pigmented bacterial strain, designated H184, was isolated from human faeces. 16S rRNA gene sequence analysis showed that strain H184 represents a member of the genus Butyricimonas. Strain H184 is related to but distinct from Butyricimonasvirosa JCM 15149 and Butyricimonasparavirosa JCM 18677, with 16S rRNA gene sequence similarities of 96.32 and 96.24 %, respectively. Strain H184 shared 90.50 % hsp60 gene sequence similarity to B. virosa JCM 15149 and B. paravirosa JCM 18677. Growth occurs between 25 and 42 °C with an optimum at 37 °C. Bile and NaCl concentration range allowing growth are 0-3.75 % and 0-1.8 %, respectively. pH range for growth is 5.5-8. The strain produced propionate as the major end product from glucose. The major cellular fatty acids of strain H184 were iso-C15 : 0 (63.5 %) and iso-C17 : 0 3-OH (12.8%). The major menaquinone of the strain was MK-10 (86 %). DNA G+C content of the isolate H184 was 44.2 mol%. The genome-based comparison between strain H184 and B. virosa JCM 15149 by pairwise average nucleotide identity indicated a clear distinction with a score of 87.22. On the basis of these data, strain H184 represents a novel species of the genus Butyricimonas, for which the name Butyricimonas faecalis sp. nov. is proposed. The type strain of B. faecalis is H184 (DSM 106867, LMG 30602).

Document type Article de périodique (Journal article)
Access type Accès libre
Publication date 2019
Language Anglais
Journal information "International journal of systematic and evolutionary microbiology" - Vol. 69, no.3, p. 833-838 (2019)
Peer reviewed yes
issn 1466-5034
e-issn 1466-5034
Publication status Publié
Affiliations UCL - SSS/LDRI - Louvain Drug Research Institute
UCL - SSS/DDUV - Institut de Duve
Keywords Butyricimonas ; human faeces ; human intestinal microbiota ; porphyromonadaceae
Links
  1. Suau, Appl Environ Microbiol, 65, 4799 (1999)
  2. Eckburg P. B., Diversity of the Human Intestinal Microbial Flora, 10.1126/science.1110591
  3. Sakamoto M., Takagaki A., Matsumoto K., Kato Y., Goto K., Benno Y., Butyricimonas synergistica gen. nov., sp. nov. and Butyricimonas virosa sp. nov., butyric acid-producing bacteria in the family 'Porphyromonadaceae' isolated from rat faeces, 10.1099/ijs.0.007674-0
  4. Duncan Sylvia H, Stewart Colin S, Hold Georgina L, Flint Harry J, Harmsen Hermie J M, Growth requirements and fermentation products of Fusobacterium prausnitzii, and a proposal to reclassify it as Faecalibacterium prausnitzii gen. nov., comb. nov., 10.1099/00207713-52-6-2141
  5. Dione N., Khelaifia S., La Scola B., Lagier J.C., Raoult D., A quasi-universal medium to break the aerobic/anaerobic bacterial culture dichotomy in clinical microbiology, 10.1016/j.cmi.2015.10.032
  6. Sizova M. V., Hohmann T., Hazen A., Paster B. J., Halem S. R., Murphy C. M., Panikov N. S., Epstein S. S., New Approaches for Isolation of Previously Uncultivated Oral Bacteria, 10.1128/aem.06813-11
  7. TURNER SEÁN, PRYER KATHLEEN M., MIAO VIVIAN P. W., PALMER JEFFREY D., Investigating Deep Phylogenetic Relationships among Cyanobacteria and Plastids by Small Subunit rRNA Sequence Analysis, 10.1111/j.1550-7408.1999.tb04612.x
  8. Sakamoto M., Suzuki N., Benno Y., hsp60 and 16S rRNA gene sequence relationships among species of the genus Bacteroides with the finding that Bacteroides suis and Bacteroides tectus are heterotypic synonyms of Bacteroides pyogenes, 10.1099/ijs.0.021154-0
  9. Sakamoto M., Ohkuma M., Usefulness of the hsp60 gene for the identification and classification of Gram-negative anaerobic rods, 10.1099/jmm.0.020420-0
  10. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies, 10.1099/ijsem.0.001755
  11. Edgar R. C., MUSCLE: multiple sequence alignment with high accuracy and high throughput, 10.1093/nar/gkh340
  12. Tamura K., Nei M., Kumar S., Prospects for inferring very large phylogenies by using the neighbor-joining method, 10.1073/pnas.0404206101
  13. The neighbor-joining method: a new method for reconstructing phylogenetic trees., 10.1093/oxfordjournals.molbev.a040454
  14. Kumar Sudhir, Stecher Glen, Tamura Koichiro, MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets, 10.1093/molbev/msw054
  15. Chin Chen-Shan, Alexander David H, Marks Patrick, Klammer Aaron A, Drake James, Heiner Cheryl, Clum Alicia, Copeland Alex, Huddleston John, Eichler Evan E, Turner Stephen W, Korlach Jonas, Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data, 10.1038/nmeth.2474
  16. Lee Imchang, Chalita Mauricio, Ha Sung-Min, Na Seong-In, Yoon Seok-Hwan, Chun Jongsik, ContEst16S: an algorithm that identifies contaminated prokaryotic genomes using 16S RNA gene sequences, 10.1099/ijsem.0.001872
  17. Lee Imchang, Ouk Kim Yeong, Park Sang-Cheol, Chun Jongsik, OrthoANI: An improved algorithm and software for calculating average nucleotide identity, 10.1099/ijsem.0.000760
  18. Tindall B.J., A Comparative Study of the Lipid Composition of Halobacterium saccharovorum from Various Sources, 10.1016/s0723-2020(11)80158-x
  19. Tindall B.J., Lipid composition ofHalobacterium lacusprofundi, 10.1111/j.1574-6968.1990.tb03996.x
  20. Miller, J Clin Microbiol, 16, 584 (1982)
  21. KUYKENDALL L. D., ROY M. A., O'NEILL J. J., DEVINE T. E., Fatty Acids, Antibiotic Resistance, and Deoxyribonucleic Acid Homology Groups of Bradyrhizobium japonicum, 10.1099/00207713-38-4-358
  22. Sakamoto M., Tanaka Y., Benno Y., Ohkuma M., Butyricimonas faecihominis sp. nov. and Butyricimonas paravirosa sp. nov., isolated from human faeces, and emended description of the genus Butyricimonas, 10.1099/ijs.0.065318-0
  23. Hahnke, Front Microbiol, 2016, 7 (2003)
Bibliographic reference Le Roy, Tiphaine ; Van Der Smissen, Patrick ; Paquot, Adrien ; Delzenne, Nathalie M. ; Muccioli, Giulio ; et. al. Butyricimonas faecalis sp. nov., isolated from human faeces and emended description of the genus Butyricimonas.. In: International journal of systematic and evolutionary microbiology, Vol. 69, no.3, p. 833-838 (2019)
Permanent URL http://hdl.handle.net/2078.1/214483