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The effect on early renal function of various dynamic preservation strategies in a preclinical pig ischemia-reperfusion autotransplant model.

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Darius, Tom [UCL] Gianello, Pierre [UCL] Vergauwen, Martial [UCL] Mourad, Nizar [UCL] Buemi, Antoine [UCL] De Meyer, Martine [UCL] Mourad, Michel [UCL]

The aims of this study were to determine the most optimal timing to start machine perfusion during kidney preservation to improve early graft function and to evaluate the impact of temperature and oxygen supply during machine perfusion in a porcine ischemia-reperfusion autotransplant model. The left kidney of an approximately 40-kg female Belgian Landrace pig was exposed to 30 minutes of warm ischemia via vascular clamping and randomized to 1 of 6 study groups: (1) 22-hour static cold storage (SCS) (n = 6), (2) 22-hour hypothermic machine perfusion (HMP) (n = 6), (3) 22-hour oxygenated HMP (n = 7), (4) 20-hour HMP plus 2-hour normothermic perfusion (NP) (n = 6), (5) 20-hour SCS plus 2-hour oxygenated HMP (n = 7), and (6) 20-hour SCS plus 2-hour NP (n = 6). Graft recovery measured by serum creatinine level was significantly faster for continuous HMP preservation strategies compared with SCS alone and for all end-ischemic strategies. The active oxygenated 22-hour HMP group demonstrated a significantly faster recovery from early graft function compared with the 22-hour nonactive oxygenated HMP group. Active oxygenation was also found to be an important modulator of a faster increase in renal flow during HMP preservation. Continuous oxygenated HMP applied from the time of kidney procurement until transplant might be the best preservation strategy to improve early graft function.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 2019
Language Anglais
Journal information "American journal of transplantation" - Vol. 19, no. 3, p. 752-762 (2019)
Peer reviewed yes
Publisher Wiley-Blackwell ((United States) Hoboken, NJ)
issn 1600-6135
e-issn 1600-6143
Publication status Publié
Affiliations UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation
UCL - (SLuc) Service de chirurgie et transplantation abdominale
Keywords Animal models: porcine ; Delayed graft function (DGF) ; Kidney (native) function/dysfunction ; Kidney transplantation/nephrology ; Organ perfusion and preservation ; Organ procurement and allocation ; Organ transplantation in general ; Translational research/science
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Bibliographic reference Darius, Tom ; Gianello, Pierre ; Vergauwen, Martial ; Mourad, Nizar ; Buemi, Antoine ; et. al. The effect on early renal function of various dynamic preservation strategies in a preclinical pig ischemia-reperfusion autotransplant model.. In: American journal of transplantation, Vol. 19, no. 3, p. 752-762 (2019)
Permanent URL http://hdl.handle.net/2078.1/212954