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In situ nanomechanical characterization of the early stages of swelling and degradation of a biodegradable polymer

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Dumitru, Andra-Cristina [UCL] Espinosa, F.M. Garcia, R. Foschi, G. Tortorella, S. Biscarini, F.

The interactions of a biodegradable scaffold with cells or living tissues depend on the time-evolution of the nanoscale properties of the scaffold. We present an in situ quantitative study on the early-stage swelling and degradation of poly(lactic-co-glycolic acid) (PLGA). A novel metrology scheme based on force microscopy measurements of the patterns of PLGA nanostructures is developed to characterize the evolution of topography, volume and nanomechanical properties. The volume and nanoscale roughness show an oscillating behaviour during the first eight days of immersion; at a later stage, we observe a continuous decrease of the volume. The effective Young's modulus exhibits a monotonic decrease from an initial value of about 2.4 GPa down to 9 MPa at day 14. The oscillating behaviour of the volume before the onset of full degradation is explained by a coupled diffusion-swelling mechanism. The appearance of a second maximum in the volume evolution results from the competition between swelling and degradation.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2015
Language Anglais
Journal information "Nanoscale" - Vol. 7, no. 12, p. 5403-5410 (2015)
Peer reviewed yes
Publication status Publié
Affiliation UCL - SST/LIBST - Louvain Institute of Biomolecular Science and Technology
Links
  1. Anderson James M, Shive Matthew S, Biodegradation and biocompatibility of PLA and PLGA microspheres, 10.1016/s0169-409x(97)00048-3
  2. Yamaguchi Kazuhiro, Anderson James M., In vivo biocompatibility studies of medisorb® 65/35 D,L-lactide/glycolide copolymer microspheres, 10.1016/0168-3659(93)90169-6
  3. Gautier Sandrine E., Oudega Martin, Fragoso Miryam, Chapon Pascal, Plant Giles W., Bunge Mary Bartlett, Parel Jean-Marie, Poly(?-hydroxyacids) for application in the spinal cord: Resorbability and biocompatibility with adult rat Schwann cells and spinal cord, 10.1002/(sici)1097-4636(19981215)42:4<642::aid-jbm22>3.0.co;2-k
  4. Ignatius A.A., Claes L.E., In vitro biocompatibility of bioresorbable polymers: poly(L, DL-lactide) and poly(L-lactide-co-glycolide), 10.1016/0142-9612(96)81421-9
  5. Ke Cherng-Jyh, Su Tzu-Yuan, Chen Hsin-Lung, Liu Hao-Li, Chiang Wei-Lun, Chu Po-Chun, Xia Younan, Sung Hsing-Wen, Smart Multifunctional Hollow Microspheres for the Quick Release of Drugs in Intracellular Lysosomal Compartments, 10.1002/anie.201102852
  6. Chen Menglin, Gao Shan, Dong Mingdong, Song Jie, Yang Chuanxu, Howard Kenneth Alan, Kjems Jørgen, Besenbacher Flemming, Chitosan/siRNA Nanoparticles Encapsulated in PLGA Nanofibers for siRNA Delivery, 10.1021/nn300106t
  7. Mathew, PLoS One, 7, 10 (2012)
  8. Hild Nora, Schneider Oliver D., Mohn Dirk, Luechinger Norman A., Koehler Fabian M., Hofmann Sandra, Vetsch Jolanda R., Thimm Benjamin W., Müller Ralph, Stark Wendelin J., Two-layer membranes of calcium phosphate/collagen/PLGA nanofibres: in vitro biomineralisation and osteogenic differentiation of human mesenchymal stem cells, 10.1039/c0nr00615g
  9. Wang Gou-Jen, Lin Yan-Cheng, Hsu Shan-hui, The fabrication of PLGA microvessel scaffolds with nano-patterned inner walls, 10.1007/s10544-010-9438-x
  10. Wu Xue Shen, Wang Nuo, Synthesis, characterization, biodegradation, and drug delivery application of biodegradable lactic/glycolic acid polymers. Part II: Biodegradation, 10.1163/156856201744425
  11. Grizzi I., Garreau H., Li S., Vert M., Hydrolytic degradation of devices based on poly(dl-lactic acid) size-dependence, 10.1016/0142-9612(95)93258-f
  12. Vey Elisabeth, Roger Caroline, Meehan Liz, Booth Jonathan, Claybourn Mike, Miller Aline F., Saiani Alberto, Degradation mechanism of poly(lactic-co-glycolic) acid block copolymer cast films in phosphate buffer solution, 10.1016/j.polymdegradstab.2008.07.018
  13. Park Tae Gwan, Degradation of poly(lactic-co-glycolic acid) microspheres: effect of copolymer composition, 10.1016/0142-9612(95)93575-x
  14. Göpferich A, Mechanisms of polymer degradation and erosion, 10.1016/0142-9612(96)85755-3
  15. Assender H., How Surface Topography Relates to Materials' Properties, 10.1126/science.1074955
  16. Goetz Jacky G., Minguet Susana, Navarro-Lérida Inmaculada, Lazcano Juan José, Samaniego Rafael, Calvo Enrique, Tello Marta, Osteso-Ibáñez Teresa, Pellinen Teijo, Echarri Asier, Cerezo Ana, Klein-Szanto Andres J.P., Garcia Ricardo, Keely Patricia J., Sánchez-Mateos Paloma, Cukierman Edna, Del Pozo Miguel A., Biomechanical Remodeling of the Microenvironment by Stromal Caveolin-1 Favors Tumor Invasion and Metastasis, 10.1016/j.cell.2011.05.040
  17. Chyasnavichyus Marius, Young Seth L., Tsukruk Vladimir V., Probing of Polymer Surfaces in the Viscoelastic Regime, 10.1021/la404925h
  18. Vey Elisabeth, Rodger Caroline, Meehan Liz, Booth Jonathan, Claybourn Mike, Miller Aline F., Saiani Alberto, The impact of chemical composition on the degradation kinetics of poly(lactic-co-glycolic) acid copolymers cast films in phosphate buffer solution, 10.1016/j.polymdegradstab.2011.12.010
  19. Pamula Elzbieta, Menaszek Elzbieta, In vitro and in vivo degradation of poly(l-lactide-co-glycolide) films and scaffolds, 10.1007/s10856-007-3292-2
  20. Perron Josee K., Naguib Hani E., Daka Joseph, Chawla Attar, Wilkins Ruth, A study on the effect of degradation media on the physical and mechanical properties of porous PLGA 85/15 scaffolds, 10.1002/jbm.b.31470
  21. Dufrêne Yves F, Martínez-Martín David, Medalsy Izhar, Alsteens David, Müller Daniel J, Multiparametric imaging of biological systems by force-distance curve–based AFM, 10.1038/nmeth.2602
  22. A. Ikai , in Nano/Micro Biotechnology, ed. I. Endo and T. Nagamune, Springer, Berlin, Heidelberg, 2010, vol. 119, pp. 47–61
  23. Herruzo Elena T., Perrino Alma P., Garcia Ricardo, Fast nanomechanical spectroscopy of soft matter, 10.1038/ncomms4126
  24. Martinez-Martin D., Herruzo E. T., Dietz C., Gomez-Herrero J., Garcia R., Noninvasive Protein Structural Flexibility Mapping by Bimodal Dynamic Force Microscopy, 10.1103/physrevlett.106.198101
  25. Chen Menglin, Nielsen Søren Roesgaard, Uyar Tamer, Zhang Shuai, Zafar Ashar, Dong Mingdong, Besenbacher Flemming, Electrospun UV-responsive supramolecular nanofibers from a cyclodextrin–azobenzene inclusion complex, 10.1039/c2tc00180b
  26. García Ricardo, San Paulo Alvaro, Attractive and repulsive tip-sample interaction regimes in tapping-mode atomic force microscopy, 10.1103/physrevb.60.4961
  27. Herruzo Elena T., Garcia Ricardo, Frequency response of an atomic force microscope in liquids and air: Magnetic versus acoustic excitation, 10.1063/1.2794426
  28. Dong Mingdong, Husale Sudhir, Sahin Ozgur, Determination of protein structural flexibility by microsecond force spectroscopy, 10.1038/nnano.2009.156
  29. Butt Hans-Jürgen, Cappella Brunero, Kappl Michael, Force measurements with the atomic force microscope: Technique, interpretation and applications, 10.1016/j.surfrep.2005.08.003
  30. Zhang Shuai, Aslan Hüsnü, Besenbacher Flemming, Dong Mingdong, Quantitative biomolecular imaging by dynamic nanomechanical mapping, 10.1039/c4cs00176a
  31. Butt H -J, Jaschke M, Calculation of thermal noise in atomic force microscopy, 10.1088/0957-4484/6/1/001
  32. Sneddon Ian N., The relation between load and penetration in the axisymmetric boussinesq problem for a punch of arbitrary profile, 10.1016/0020-7225(65)90019-4
  33. Korsmeyer Richard W., Peppas Nikolaos A., Solute and penetrant diffusion in swellable polymers. III. Drug release from glassy poly(HEMA-co-NVP) copolymers, 10.1016/0168-3659(84)90001-4
  34. Alfrey Turner, Gurnee E. F., Lloyd W. G., Diffusion in glassy polymers, 10.1002/polc.5070120119
  35. Vrentas J. S., Jarzebski C. M., Duda J. L., A Deborah number for diffusion in polymer-solvent systems, 10.1002/aic.690210510
  36. http://www.comsol.com
  37. Gualandi Chiara, Govoni Marco, Foroni Laura, Valente Sabrina, Bianchi Michele, Giordano Emanuele, Pasquinelli Gianandrea, Biscarini Fabio, Focarete Maria Letizia, Ethanol disinfection affects physical properties and cell response of electrospun poly(l-lactic acid) scaffolds, 10.1016/j.eurpolymj.2012.09.016
  38. Caldorera-Moore Mary, Kang Min Kyoo, Moore Zachary, Singh Vikramjit, Sreenivasan S. V., Shi Li, Huang Rui, Roy Krishnendu, Swelling behavior of nanoscale, shape- and size-specific, hydrogel particles fabricated using imprint lithography, 10.1039/c0sm01185a
  39. Samadi N., Abbadessa A., Di Stefano A., van Nostrum C.F., Vermonden T., Rahimian S., Teunissen E.A., van Steenbergen M.J., Amidi M., Hennink W.E., The effect of lauryl capping group on protein release and degradation of poly(d,l-lactic-co-glycolic acid) particles, 10.1016/j.jconrel.2013.05.034
  40. Semler E.J., Tjia J.S., Moghe P.V., Analysis of Surface Microtopography of Biodegradable Polymer Matrices Using Confocal Reflection Microscopy, 10.1021/bp9700871
  41. Pamula Elzbieta, Kokoszka Justyna, Cholewa-Kowalska Katarzyna, Laczka Maria, Kantor Lukasz, Niedzwiedzki Lukasz, Reilly Gwendolen C., Filipowska Joanna, Madej Wojciech, Kolodziejczyk Malgorzata, Tylko Grzegorz, Osyczka Anna M., Degradation, Bioactivity, and Osteogenic Potential of Composites Made of PLGA and Two Different Sol–Gel Bioactive Glasses, 10.1007/s10439-011-0307-4
  42. Vidović Elvira, Klee Doris, Höcker Hartwig, Evaluation of water uptake and mechanical properties of biomedical polymers, 10.1002/app.39624
Bibliographic reference Dumitru, Andra-Cristina ; Espinosa, F.M. ; Garcia, R. ; Foschi, G. ; Tortorella, S. ; et. al. In situ nanomechanical characterization of the early stages of swelling and degradation of a biodegradable polymer. In: Nanoscale, Vol. 7, no. 12, p. 5403-5410 (2015)
Permanent URL http://hdl.handle.net/2078.1/211859