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Interleukin-22-deficiency and microbiota contribute to the exacerbation of Toxoplasma gondii-induced intestinal inflammation.

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Couturier-Maillard, Aurélie Froux, Nathalie Piotet-Morin, Julie Michaudel, Chloé Brault, Léa Dumoutier, Laure [UCL] Renauld, Jean-Christophe [UCL] Ryffel, Bernhard

Upon oral infection with Toxoplasma gondii cysts (76 K strain) tachyzoites are released into the intestinal lumen and cross the epithelial barrier causing damage and acute intestinal inflammation in C57BL/6 (B6) mice. Here we investigated the role of microbiota and IL-22 in T.gondii-induced small intestinal inflammation. Oral T.gondii infection in B6 mice causes inflammation with IFNγ and IL-22 production. In IL-22-deficient mice, T.gondii infection augments the Th1 driven inflammation. Deficiency in either IL-22bp, the soluble IL-22 receptor or Reg3γ, an IL-22-dependent antimicrobial lectin/peptide, did not reduce inflammation. Under germ-free conditions, T.gondii-induced inflammation was reduced in correlation with parasite load. But intestinal inflammation is still present in germ-free mice, at low level, in the lamina propria, independently of IL-22 expression. Exacerbated intestinal inflammation driven by absence of IL-22 appears to be independent of IL-22 deficiency associated-dysbiosis as similar inflammation was observed after fecal transplantation of IL-22 or WT microbiota to germ-free-WT mice. Our results suggest cooperation between parasite and intestinal microbiota in small intestine inflammation development and endogenous IL-22 seems to exert a protective role independently of its effect on the microbiota. In conclusion, IL-22 participates in T.gondii induced acute small intestinal inflammation independently of microbiota and Reg3γ.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 2018
Language Anglais
Journal information "Mucosal Immunology" - Vol. 11, no. 4, p. 1181-1190 (2018)
Peer reviewed yes
Publisher Nature Publishing Group ((United States) New York, NY)
issn 1933-0219
e-issn 1935-3456
Publication status Publié
Affiliation UCL - SSS/DDUV/MEXP - Médecine expérimentale
Links
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Bibliographic reference Couturier-Maillard, Aurélie ; Froux, Nathalie ; Piotet-Morin, Julie ; Michaudel, Chloé ; Brault, Léa ; et. al. Interleukin-22-deficiency and microbiota contribute to the exacerbation of Toxoplasma gondii-induced intestinal inflammation.. In: Mucosal Immunology, Vol. 11, no. 4, p. 1181-1190 (2018)
Permanent URL http://hdl.handle.net/2078.1/199962