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Evaluation of a new meropenem-EDTA double-ended Etest strip for the detection of the cfiA metallo-beta-lactamase gene in clinical isolates of Bacteroides fragilis.

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Bogaerts, Pierre [UCL] Glupczynski, Gerald [UCL] Engelhardt, A. Berhin, C. Bylund, L. Yusof, A.

Thirty-five Bacteroides fragilis clinical isolates with varying susceptibility to meropenem were analysed with a prototype of a double-ended Etest strip containing meropenem +/- EDTA, designed for the detection of the CfiA metallo-beta-lactamase. Phenotypic results obtained with this new Etest strip were related to the genotype and compared to the results of the Etest containing imipenem +/- EDTA. Whereas the Etest with imipenem +/- EDTA only allowed detection of isolates with high-level resistance (both MICs of imipenem and meropenem >32 mg/L), reflecting the possible underestimation of CfiA prevalence in B. fragilis, the Etest with meropenem +/- EDTA proved to be more accurate, particularly for isolates with low-level carbapenem resistance, suggesting its potential for broader detection of CfiA production.

Document type Article de périodique (Journal article) – Evaluation Studies, Journal Article
Access type Accès restreint
Publication date 2008
Journal information "Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases" - Vol. 14, no. 10, p. 973-7 (2008)
Peer reviewed yes
e-issn 1469-0691
issn 1198-743X
Publication status Publié
Affiliations UCL
UCL - (MGD) Laboratoire de biologie clinique
MESH Subject beta-Lactamases - analysis ; beta-Lactam Resistance ; Thienamycins - pharmacology ; Microbial Sensitivity Tests ; Humans ; Genotype ; Genes, Bacterial ; DNA, Bacterial - genetics ; Bacteroides fragilis - enzymology - isolation & purification ; Bacteroides Infections - microbiology ; Bacterial Proteins - analysis ; Anti-Bacterial Agents - pharmacology
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Bibliographic reference Bogaerts, Pierre ; Glupczynski, Gerald ; Engelhardt, A. ; Berhin, C. ; Bylund, L. ; et. al. Evaluation of a new meropenem-EDTA double-ended Etest strip for the detection of the cfiA metallo-beta-lactamase gene in clinical isolates of Bacteroides fragilis.. In: Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases, Vol. 14, no. 10, p. 973-7 (2008)
Permanent URL http://hdl.handle.net/2078.1/19601