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Characterization of alpha,alpha-trehalase released in the intestinal lumen by the probiotic Saccharomyces boulardii.

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Authors :
Document type : Article de périodique (Journal article) – Journal Article
Abstract :

Objective. Trehalose intolerance due to alpha,alpha-trehalase deficiency has scarcely been studied. The purpose of this study was to measure alpha,alpha-trehalase activity in intestinal biopsy samples from 200 consecutive patients over a period of 6 months, and to characterize alpha,alpha-trehalase released by the probiotic Saccharomyces boulardii (S. boulardii). Material and methods. Enzyme activities were measured in human and rat intestinal mucosal samples using the micromethod of Messer & Dalqvist. alpha,alpha-trehalase from S. boulardii was immunoprecipitated and Western blotted using an IgG purified antibody raised against a 23 amino acid peptide of alpha,alpha-trehalase of S. cerevisiae. Results. Among 200 patients, most of whom complained of abdominal symptoms and diarrhoea, 18 (9%) had total alpha,alpha-trehalase deficiency (0-12 U/g mucosa) and 39 had partial deficiency (3-12 U/g mucosa). Only 4 patients (2%) presented selective alpha,alpha-trehalase deficiency with otherwise normal disaccharidases. Expressed per gram of powder, alpha,alpha-trehalase from S. boulardii delivered in vitro an activity 175 times higher than that of human trehalase per gram of intestinal mucosa. V(max) (22+/-0.43 micromol) and K(m) (5 mM) were close to that of the human enzyme, whereas Western blot revealed a signal of two subunits of 82 kDa. Finally, treatment of rats with S. boulardii resulted in increases in alpha,alpha-trehalase activities of 25 to 45% (p<0.01) in endoluminal fluid and intestinal mucosa compared with in controls. Conclusions. Our data suggest that alpha,alpha-trehalase deficiency is more common than is believed and that oral administration of S. boulardii could be beneficial in patients with digestive symptoms caused by trehalose intolerance.
Publication date : 2008
Journal information : "Scandinavian journal of gastroenterology" - Vol. 43, no. 12, p. 1489-96 (2008)
Peer reviewed : yes
e-issn : 1502-7708
issn : 0085-5928
Publication status : Publié
Affiliation : UCL - MD/GYPE - Département de gynécologie, d'obstétrique et de pédiatrie
MESH Subject : Trehalase - metabolism ; Saccharomyces - enzymology ; Rats ; Probiotics - metabolism ; Middle Aged ; Male ; Intestines - metabolism ; Humans ; Female ; Animals ; Adult
Links :
Bibliographic reference Buts, Jean-Paul ; Stilmant, Catherine ; Bernasconi, Paul ; Neirinck, Christiane ; Dekeyser, Nadine. Characterization of alpha,alpha-trehalase released in the intestinal lumen by the probiotic Saccharomyces boulardii.. In: Scandinavian journal of gastroenterology, Vol. 43, no. 12, p. 1489-96 (2008)
Permanent URL http://hdl.handle.net/2078.1/19563

References Provided by I4OC

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