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Protein-polyelectrolyte complexes to improve the biological activity of proteins in layer-by-layer assemblies

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vander Straeten, Aurélien [UCL] Bratek-Skicki, Anna [UCL] Germain, Loïc [UCL] D'Haese, Cécile [UCL] Eloy, Pierre [UCL] Fustin, Charles-André [UCL] Dupont-Gillain, Christine C. [UCL]

A standard method of protein immobilization is proposed, based on the use of protein–polyelectrolyte complexes (PPCs) as building blocks for layer-by-layer assembly. Thicker multilayers, with a higher polyelectrolyte fraction, are obtained with PPCs compared to single protein molecules. Biological activity is not only maintained, but specific activity is also higher, as demonstrated for lysozyme–poly(styrene sulfonate) complexes. This is attributed to the more hydrated state of the assemblies. This new method of protein immobilization opens up perspectives for biotechnology and biomedical applications.

Document type Article de périodique (Journal article)
Access type Accès restreint
Publication date 2017
Language Anglais
Journal information "Nanoscale" - Vol. 9, p. 17186-17192 (2017)
Peer reviewed yes
Publisher National Center for Nanoscience and Technology
issn 2040-3364
e-issn 2040-3372
Publication status Publié
Affiliation UCL - SST/IMCN/BSMA - Bio and soft matter
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Bibliographic reference vander Straeten, Aurélien ; Bratek-Skicki, Anna ; Germain, Loïc ; D'Haese, Cécile ; Eloy, Pierre ; et. al. Protein-polyelectrolyte complexes to improve the biological activity of proteins in layer-by-layer assemblies. In: Nanoscale, Vol. 9, p. 17186-17192 (2017)
Permanent URL http://hdl.handle.net/2078.1/189073