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Characterization of upper lamina propria interstitial cells in bladders from patients with neurogenic detrusor overactivity and bladder pain syndrome

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Gevaert, Thomas De Vos, Rita Everaerts, Wouter Libbrecht, Louis [UCL] Van Der Aa, Frank De Ridder, Dirk

The upper lamina propria (ULP) area of interstitial cells (IC) in bladder has been studied for more than a decade in several species including human beings. Nevertheless there is still lack of uniformity in terminology of this cell layer. The aim of the present study was to add new data to the morphological and immunohistochemical phenotype of these cells and to find out whether this phenotype is changed in bladders from patients with neurogenic detrusor overactivity (NDO) and bladder pain syndrome (BPS). Bladder tissue was obtained from a control group and from patients with NDO and BPS. Samples were processed for morphology, electron microscopy and immunohistochemistry. A morphological and immunohistochemical phenotype for the ULP IC was assessed and changes in this phenotype were looked for in samples from patients with NDO and BPS. The ULP IC were characterized ultrastructurally by the presence of actin filaments with densifications, many caveolae and abundant rough endoplasmic reticulum (RER); on immunohistochemistry ULP IC were immunoreactive for α-sma, vimentin, CD10 and podoplanin and categorized as interstitial Cajal-like cells (ICLC). In NDO and BPS bladders we found a phenotypical shift towards a fibroblastic phenotype which was even more pronounced in the NDO group. In both groups there was also an increased presence in ULP lymphocytes. The ULP area in the human bladder contains a population of ICLC with distinct ultrastructural morphology and immunohistochemical phenotype. Their unique α-sma(+) /desmin(-) /CD34(-) phenotype allows studying this population in various bladder disorders. In bladders form patients with BPS and NDO, we observed these ULP ICLC to shift towards a fibroblast phenotype.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 2011
Language Anglais
Journal information "Journal of Cellular and Molecular Medicine" - Vol. 15, no.12, p. 2586-2593 (2011)
Peer reviewed yes
Publisher Wiley-Blackwell Publishing Ltd. ((United Kingdom) Oxford)
issn 1582-1838
e-issn 1582-4934
Publication status Publié
Affiliation [UGent]
MESH Subject Adult ; Urinary Bladder ; Urinary Bladder, Neurogenic ; Urinary Bladder, Overactive ; Cystitis, Interstitial ; Female ; Humans ; Interstitial Cells of Cajal ; Male ; Middle Aged ; Mucous Membrane ; Phenotype
Keywords Bladder ; Neurogenic detrusor overactivity ; Bladder pain syndrome ; Interstitial cell ; Myofibroblast ; Lamina propria
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Bibliographic reference Gevaert, Thomas ; De Vos, Rita ; Everaerts, Wouter ; Libbrecht, Louis ; Van Der Aa, Frank ; et. al. Characterization of upper lamina propria interstitial cells in bladders from patients with neurogenic detrusor overactivity and bladder pain syndrome. In: Journal of Cellular and Molecular Medicine, Vol. 15, no.12, p. 2586-2593 (2011)
Permanent URL http://hdl.handle.net/2078/187536