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Direct Evidence of the Link Between Energetic Metabolism and Proliferation Capacity of Cancer Cells In Vitro.

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De Preter, Géraldine [UCL] Danhier, Pierre [UCL] Porporato, Paolo [UCL] Payen, Valery L [UCL] Jordan, Benedicte [UCL] Sonveaux, Pierre [UCL] Gallez, Bernard [UCL]

The aim of the study was to assess the link between the metabolic profile and the proliferation capacity of a range of human and murine cancer cell lines. First, the combination of mitochondrial respiration and glycolytic efficiency measurements allowed the determination of different metabolic profiles among the cell lines, ranging from a mostly oxidative to a mostly glycolytic phenotype. Second, the study revealed that cell proliferation, evaluated by DNA synthesis measurements, was statistically correlated to glycolytic efficiency. This indicated that glycolysis is the key energetic pathway linked to cell proliferation rate. Third, to validate this hypothesis and exclude non-metabolic factors, mitochondria-depleted were compared to wild-type cancer cells, and the data showed that enhanced glycolysis observed in mitochondria-depleted cells is also associated with an increase in proliferation capacity.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2016
Language Anglais
Journal information "Advances in Experimental Medicine and Biology" - Vol. 876, p. 209-14 (2016)
Peer reviewed yes
Publisher Springer New York LLC (New York)
issn 0065-2598
Publication status Publié
Affiliations UCL - SSS/LDRI - Louvain Drug Research Institute
UCL - SSS/IREC/FATH - Pôle de Pharmacologie et thérapeutique
MESH Subject Animals ; Oxygen Consumption ; Cell Line, Tumor ; Cell Proliferation ; Energy Metabolism ; Glycolysis ; Humans ; Mice ; Mitochondria ; Neoplasms
Links
  1. Warburg Otto, über den Stoffwechsel der Carcinomzelle, 10.1007/bf01726151
  2. Penta John S., Johnson F.M., Wachsman Joseph T., Copeland William C., Mitochondrial DNA in human malignancy, 10.1016/s1383-5742(01)00053-9
  3. Xu Lei, Tong Ricky, Cochran David M., Jain Rakesh K., Blocking Platelet-Derived Growth Factor-D/Platelet-Derived Growth Factor Receptor β Signaling Inhibits Human Renal Cell Carcinoma Progression in an Orthotopic Mouse Model, 10.1158/0008-5472.can-04-4313
  4. Moreno-Sánchez Rafael, Marín-Hernández Alvaro, Saavedra Emma, Pardo Juan P., Ralph Stephen J., Rodríguez-Enríquez Sara, Who controls the ATP supply in cancer cells? Biochemistry lessons to understand cancer energy metabolism, 10.1016/j.biocel.2014.01.025
  5. Moreno-Sánchez Rafael, Rodríguez-Enríquez Sara, Marín-Hernández Alvaro, Saavedra Emma, Energy metabolism in tumor cells : Glycolytic and mitochondrial metabolism of tumor cells, 10.1111/j.1742-4658.2007.05686.x
  6. Zu Xin Lin, Guppy Michael, Cancer metabolism: facts, fantasy, and fiction, 10.1016/j.bbrc.2003.11.136
  7. Diepart Caroline, Verrax Julien, Calderon Pedro Buc, Feron Olivier, Jordan Bénédicte F., Gallez Bernard, Comparison of methods for measuring oxygen consumption in tumor cells in vitro, 10.1016/j.ab.2009.09.029
  8. Taper HS, Woolley GW, Teller MN et al (1966) A new transplantable mouse liver tumor of spontaneous origin. Cancer Res 26:143–148
  9. Volpe John P., Hunter Nancy, Basic Ivan, Milas Luka, Metastatic properties of murine sarcomas and carcinomas I. Positive correlation with lung colonization and lack of correlation with s.c. tumor take, 10.1007/bf01585082
  10. Rockwell S, Kallman RF (1972) Growth and cell population kinetics of single and multiple KHT sarcomas. Cell Tissue Kinet 5:449–457
  11. Reilly RT, Gottlieb MB, Ercolini AM et al (2000) HER-2/neu is a tumor rejection target in tolerized HER-2/neu transgenic mice. Cancer Res 60:3569–3576
  12. King M., Attardi G, Human cells lacking mtDNA: repopulation with exogenous mitochondria by complementation, 10.1126/science.2814477
  13. James Philip E., Jackson Simon K., Grinberg Oleg Y., Swartz Harold M., The effects of endotoxin on oxygen consumption of various cell types in vitro: An EPR oximetry study, 10.1016/0891-5849(94)00179-n
  14. Jordan BF, Gregoire V, Demeure RJ et al (2002) Insulin increases the sensitivity of tumors to irradiation: involvement of an increase in tumor oxygenation mediated by a nitric oxide-dependent decrease of the tumor cells oxygen consumption. Cancer Res 62:3555–3561
  15. Fogal V., Richardson A. D., Karmali P. P., Scheffler I. E., Smith J. W., Ruoslahti E., Mitochondrial p32 Protein Is a Critical Regulator of Tumor Metabolism via Maintenance of Oxidative Phosphorylation, 10.1128/mcb.01101-09
  16. Lunt Sophia Y., Vander Heiden Matthew G., Aerobic Glycolysis: Meeting the Metabolic Requirements of Cell Proliferation, 10.1146/annurev-cellbio-092910-154237
  17. Books Received, 10.1093/jnci/62.3.co3
  18. Hedeskov C. J., Early effects of phytohaemagglutinin on glucose metabolism of normal human lymphocytes, 10.1042/bj1100373
  19. Tannock I F, The relation between cell proliferation and the vascular system in a transplanted mouse mammary tumour, 10.1038/bjc.1968.34
Bibliographic reference De Preter, Géraldine ; Danhier, Pierre ; Porporato, Paolo ; Payen, Valery L ; Jordan, Benedicte ; et. al. Direct Evidence of the Link Between Energetic Metabolism and Proliferation Capacity of Cancer Cells In Vitro.. In: Advances in Experimental Medicine and Biology, Vol. 876, p. 209-14 (2016)
Permanent URL http://hdl.handle.net/2078.1/186009