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A human intestinal M-cell-like model for investigating particle, antigen and microorganism translocation.

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Beloqui Garcia, Ana [UCL] Brayden, David J Artursson, Per Préat, Véronique [UCL] des Rieux, Anne [UCL]

The specialized microfold cells (M cells) in the follicle-associated epithelium (FAE) of intestinal Peyer's patches serve as antigen-sampling cells of the intestinal innate immune system. Unlike 'classical' enterocytes, they are able to translocate diverse particulates without digesting them. They act as pathways for microorganism invasion and mediate food tolerance by transcellular transport of intestinal microbiota and antigens. Their ability to transcytose intact particles can be used to develop oral drug delivery and oral immunization strategies. This protocol describes a reproducible and versatile human M-cell-like in vitro model. This model can be exploited to evaluate M-cell transport of microparticles and nanoparticles for protein, drug or vaccine delivery and to study bacterial adherence and translocation across M cells. The inverted in vitro M-cell model consists of three main steps. First, Caco-2 cells are seeded at the apical side of the inserts. Second, the inserts are inverted and B lymphocytes are seeded at the basolateral side of the inserts. Third, the conversion to M cells is assessed. Although various M-cell culture systems exist, this model provides several advantages over the rest: (i) it is based on coculture with well-established differentiated human cell lines; (ii) it is reproducible under the conditions described herein; (iii) it can be easily mastered; and (iv) it does not require the isolation of primary cells or the use of animals. The protocol requires skills in cell culture and microscopy analysis. The model is obtained after 3 weeks, and transport experiments across the differentiated model can be carried out over periods of up to 10 h.

Document type Article de périodique (Journal article) – Article de recherche
Publication date 2017
Language Anglais
Journal information "Nature Protocols (Online) : recipes for researchers" - Vol. 12, no.7, p. 1387-1399 (2017)
Peer reviewed yes
Publisher Nature Publishing Group (London)
issn 1754-2189
e-issn 1750-2799
Publication status Publié
Affiliation UCL - SSS/LDRI - Louvain Drug Research Institute
Links
  1. Hubatsch Ina, Ragnarsson Eva G E, Artursson Per, Determination of drug permeability and prediction of drug absorption in Caco-2 monolayers, 10.1038/nprot.2007.303
  2. des Rieux Anne, Fievez Virginie, Garinot Marie, Schneider Yves-Jacques, Préat Véronique, Nanoparticles as potential oral delivery systems of proteins and vaccines: A mechanistic approach, 10.1016/j.jconrel.2006.08.013
  3. Brayden David J., Jepson Mark A., Baird Alan W., Keynote review: Intestinal Peyer's patch M cells and oral vaccine targeting, 10.1016/s1359-6446(05)03536-1
  4. Brayden David J., Baird Alan W., Microparticle vaccine approaches to stimulate mucosal immunisation, 10.1016/s1286-4579(01)01445-9
  5. Kerneis S., Conversion by Peyer's Patch Lymphocytes of Human Enterocytes into M Cells that Transport Bacteria, 10.1126/science.277.5328.949
  6. Gullberg Elisabet, Leonard Maurice, Karlsson Johan, Hopkins Ann M., Brayden David, Baird Alan W., Artursson Per, Expression of Specific Markers and Particle Transport in a New Human Intestinal M-Cell Model, 10.1006/bbrc.2000.4038
  7. KIMURA Shunsuke, KISHIMOTO Ayuko, MUTOH Mami, TAKAHASHI-IWANAGA Hiromi, IWANAGA Toshihiko, GP2-expressing cells in the conjunctiva and tear ducts of mice: identification of a novel type of cells in the squamous stratified epithelium , 10.2220/biomedres.36.263
  8. Miyake Masateru, Ragnarsson Eva, Nakai Daisuke, Artursson Per, The Pro-inflammatory Cytokine Interleukin-6 Regulates Nanoparticle Transport Across Model Follicle-Associated Epithelium Cells, 10.1016/j.xphs.2016.03.043
  9. Rieux Anne des, Ragnarsson Eva G.E., Gullberg Elisabet, Préat Véronique, Schneider Yves-Jacques, Artursson Per, Transport of nanoparticles across an in vitro model of the human intestinal follicle associated epithelium, 10.1016/j.ejps.2005.04.015
  10. des Rieux Anne, Fievez Virginie, Théate Ivan, Mast Jan, Préat Véronique, Schneider Yves-Jacques, An improved in vitro model of human intestinal follicle-associated epithelium to study nanoparticle transport by M cells, 10.1016/j.ejps.2006.12.006
  11. Ohno Hiroshi, Intestinal M cells, 10.1093/jb/mvv121
  12. des Rieux Anne, Pourcelle Vincent, Cani Patrice D., Marchand-Brynaert Jacqueline, Préat Véronique, Targeted nanoparticles with novel non-peptidic ligands for oral delivery, 10.1016/j.addr.2013.01.002
  13. Gullberg E., Keita A. V., Salim S. Y., Andersson M., Caldwell K. D., Soderholm J. D., Artursson P., Identification of Cell Adhesion Molecules in the Human Follicle-Associated Epithelium That Improve Nanoparticle Uptake into the Peyer's Patches, 10.1124/jpet.106.107847
  14. Lo D., Cell culture modeling of specialized tissue: identification of genes expressed specifically by follicle-associated epithelium of Peyer's patch by expression profiling of Caco-2/Raji co-cultures, 10.1093/intimm/dxh011
  15. Araújo Francisca, Pereira Carla, Costa Joana, Barrias Cristina, Granja Pedro L., Sarmento Bruno, In vitroM-like cells genesis through a tissue-engineered triple-culture intestinal model : M-LIKE CELLSIN VITROGENESIS FOR INTESTINAL TISSUE MODEL, 10.1002/jbm.b.33508
  16. Schimpel Christa, Teubl Birgit, Absenger Markus, Meindl Claudia, Fröhlich Eleonore, Leitinger Gerd, Zimmer Andreas, Roblegg Eva, Development of an Advanced Intestinal in Vitro Triple Culture Permeability Model To Study Transport of Nanoparticles, 10.1021/mp400507g
  17. Gonzalez-Hernandez Mariam B., Liu Thomas, Blanco Luz P., Auble Heather, Payne Hilary C., Wobus Christiane E., Murine Norovirus Transcytosis across anIn VitroPolarized Murine Intestinal Epithelial Monolayer Is Mediated by M-Like Cells, 10.1128/jvi.02378-13
  18. Miyazawa K., Kanaya T., Takakura I., Tanaka S., Hondo T., Watanabe H., Rose M. T., Kitazawa H., Yamaguchi T., Katamine S., Nishida N., Aso H., Transcytosis of Murine-Adapted Bovine Spongiform Encephalopathy Agents in an In Vitro Bovine M Cell Model, 10.1128/jvi.00969-10
  19. Banerjee Amrita, Qi Jianping, Gogoi Rohan, Wong Jessica, Mitragotri Samir, Role of nanoparticle size, shape and surface chemistry in oral drug delivery, 10.1016/j.jconrel.2016.07.051
  20. Ragnarsson Eva G E, Schoultz Ida, Gullberg Elisabet, Carlsson Anders H, Tafazoli Farideh, Lerm Maria, Magnusson Karl-Eric, Söderholm Johan D, Artursson Per, Yersinia pseudotuberculosis induces transcytosis of nanoparticles across human intestinal villus epithelium via invasin-dependent macropinocytosis, 10.1038/labinvest.2008.86
  21. des Rieux Anne, Fievez Virginie, Momtaz Maryam, Detrembleur Christophe, Alonso-Sande Maria, Van Gelder Jan, Cauvin Annick, Schneider Yves-Jacques, Préat Véronique, Helodermin-loaded nanoparticles: Characterization and transport across an in vitro model of the follicle-associated epithelium, 10.1016/j.jconrel.2006.12.023
  22. Slütter Bram, Plapied Laurence, Fievez Virgine, Alonso Sande Maria, des Rieux Anne, Schneider Yves-Jacques, Van Riet Elly, Jiskoot Wim, Préat Véronique, Mechanistic study of the adjuvant effect of biodegradable nanoparticles in mucosal vaccination, 10.1016/j.jconrel.2009.05.011
  23. Garinot Marie, Fiévez Virginie, Pourcelle Vincent, Stoffelbach François, des Rieux Anne, Plapied Laurence, Theate Ivan, Freichels Hélène, Jérôme Christine, Marchand-Brynaert Jacqueline, Schneider Yves-Jacques, Préat Véronique, PEGylated PLGA-based nanoparticles targeting M cells for oral vaccination, 10.1016/j.jconrel.2007.04.021
  24. Fievez V., Plapied L., Plaideau C., Legendre D., des Rieux A., Pourcelle V., Freichels H., Jérôme C., Marchand J., Préat V., Schneider Y.J., In vitro identification of targeting ligands of human M cells by phage display, 10.1016/j.ijpharm.2010.04.023
  25. Finn Rebecca, Ahmad Tauseef, Coffey Eleanor T., Brayden David J., Baird Alan W., Boyd Aoife, Translocation ofVibrio parahaemolyticusacross anin vitroM cell model, 10.1111/1574-6968.12323
  26. Curzer Howard J., Perry Gad, Wallace Mark C., Perry Dan, The Three Rs of Animal Research: What they Mean for the Institutional Animal Care and Use Committee and Why, 10.1007/s11948-015-9659-8
  27. de Lau W., Kujala P., Schneeberger K., Middendorp S., Li V. S. W., Barker N., Martens A., Hofhuis F., DeKoter R. P., Peters P. J., Nieuwenhuis E., Clevers H., Peyer's Patch M Cells Derived from Lgr5+ Stem Cells Require SpiB and Are Induced by RankL in Cultured "Miniguts", 10.1128/mcb.00434-12
  28. Wood Megan B., Rios Daniel, Williams Ifor R., TNF-α augments RANKL-dependent intestinal M cell differentiation in enteroid cultures, 10.1152/ajpcell.00108.2016
  29. Coco Régis, Plapied Laurence, Pourcelle Vincent, Jérôme Christine, Brayden David J., Schneider Yves-Jacques, Préat Véronique, Drug delivery to inflamed colon by nanoparticles: Comparison of different strategies, 10.1016/j.ijpharm.2012.07.017
  30. Velin A K, Increased antigen and bacterial uptake in follicle associated epithelium induced by chronic psychological stress in rats, 10.1136/gut.2003.028506
  31. Beloqui Ana, des Rieux Anne, Préat Véronique, Mechanisms of transport of polymeric and lipidic nanoparticles across the intestinal barrier, 10.1016/j.addr.2016.04.014
  32. Siissalo Sanna, Laitinen Leena, Koljonen Maija, Vellonen Kati-Sisko, Kortejärvi Hanna, Urtti Arto, Hirvonen Jouni, Kaukonen Ann Marie, Effect of cell differentiation and passage number on the expression of efflux proteins in wild type and vinblastine-induced Caco-2 cell lines, 10.1016/j.ejpb.2007.03.017
  33. Makino Kozo, Oshima Kenshiro, Kurokawa Ken, Yokoyama Katsushi, Uda Takayuki, Tagomori Kenichi, Iijima Yoshio, Najima Masatomo, Nakano Masayuki, Yamashita Atsushi, Kubota Yoshino, Kimura Shigenobu, Yasunaga Teruo, Honda Takeshi, Shinagawa Hideo, Hattori Masahira, Iida Tetsuya, Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V cholerae, 10.1016/s0140-6736(03)12659-1
  34. Cedervall T., Lynch I., Lindman S., Berggard T., Thulin E., Nilsson H., Dawson K. A., Linse S., Understanding the nanoparticle-protein corona using methods to quantify exchange rates and affinities of proteins for nanoparticles, 10.1073/pnas.0608582104
  35. Both N J de, Vermey M, Dinjens W N, Bosman F T, A comparative evaluation of various invasion assays testing colon carcinoma cell lines, 10.1038/sj.bjc.6690790
  36. Beloqui Ana, Solins Mara ngeles, Gascn Alicia R., del Pozo-Rodrguez Ana, des Rieux Anne, Prat Vronique, Mechanism of transport of saquinavir-loaded nanostructured lipid carriers across the intestinal barrier, 10.1016/j.jconrel.2012.12.021
  37. Artursson Per, Palm Katrin, Luthman Kristina, Caco-2 monolayers in experimental and theoretical predictions of drug transport1PII of original article: S0169-409X(96)00415-2. The article was originally published in Advanced Drug Delivery Reviews 22 (1996) 67–84.1, 10.1016/s0169-409x(00)00128-9
  38. Carradori Dario, Barreau Kristell, Eyer Joël, The carbocyanine dye DiD labels in vitro and in vivo neural stem cells of the subventricular zone as well as myelinated structures following in vivo injection in the lateral ventricle : DiD Labels In Vitro and In Vivo Neural Stem Cells, 10.1002/jnr.23694
  39. Clark L., Martinez-Argudo I., Humphrey T. J., Jepson M. A., GFP plasmid-induced defects in Salmonella invasion depend on plasmid architecture, not protein expression, 10.1099/mic.0.025700-0
Bibliographic reference Beloqui Garcia, Ana ; Brayden, David J ; Artursson, Per ; Préat, Véronique ; des Rieux, Anne. A human intestinal M-cell-like model for investigating particle, antigen and microorganism translocation.. In: Nature Protocols (Online) : recipes for researchers, Vol. 12, no.7, p. 1387-1399 (2017)
Permanent URL http://hdl.handle.net/2078.1/185558