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Different dose rate-dependent responses of human melanoma cells and fibroblasts to low dose fast neutrons.

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Dionet, Claude Müller-Barthélémy, Melanie Marceau, Geoffroy Denis, Jean-Marc [UCL] Averbeck, Dietrich Gueulette, John [UCL] Verrelle, Pierre

PURPOSE: To analyze the dose rate influence in hyper-radiosensitivity (HRS) of human melanoma cells to very low doses of fast neutrons and to compare to the behaviour of normal human skin fibroblasts. MATERIALS AND METHODS: We explored different neutron dose rates as well as possible implication of DNA double-strand breaks (DSB), apoptosis, and energy-provider adenosine-triphosphate (ATP) levels during HRS. RESULTS: HRS in melanoma cells appears only at a very low dose rate (VLDR), while a high dose rate (HDR) induces an initial cell-radioresistance (ICRR). HRS does not seem to be due either to DSB or to apoptosis. Both phenomena (HRS and ICRR) appear to be related to ATP availability for triggering cell repair. Fibroblast survival after neutron irradiation is also dose rate-dependent but without HRS. CONCLUSIONS: Melanoma cells or fibroblasts exert their own survival behaviour at very low doses of neutrons, suggesting that in some cases there is a differential between cancer and normal cells radiation responses. Only the survival of fibroblasts at HDR fits the linear no-threshold model. This new insight into human cell responses to very low doses of neutrons, concerns natural radiations, surroundings of accelerators, proton-therapy devices, flights at high altitude. Furthermore, ATP inhibitors could increase HRS during high-linear energy transfer (high-LET) irradiation.

Document type Article de périodique (Journal article) – Article de recherche
Publication date 2016
Language Anglais
Journal information "International Journal of Radiation Biology" - Vol. 92, no.9, p. 527-535 (2016)
Peer reviewed yes
Publisher Informa Healthcare ((United Kingdom) London)
issn 0955-3002
e-issn 1362-3095
Publication status Publié
Affiliations UCL - SSS/IREC/MIRO - Pôle d'imagerie moléculaire, radiothérapie et oncologie
UCL - (SLuc) Service de radiothérapie oncologique
Keywords ATP ; Dose rates ; Fibroblasts ; Low-dose neutrons ; Melanoma ; Radioprotection
Links
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Bibliographic reference Dionet, Claude ; Müller-Barthélémy, Melanie ; Marceau, Geoffroy ; Denis, Jean-Marc ; Averbeck, Dietrich ; et. al. Different dose rate-dependent responses of human melanoma cells and fibroblasts to low dose fast neutrons.. In: International Journal of Radiation Biology, Vol. 92, no.9, p. 527-535 (2016)
Permanent URL http://hdl.handle.net/2078.1/184393