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The antifungal caspofungin increases fluoroquinolone activity against Staphylococcus aureus biofilms by inhibiting N-acetylglucosamine transferase

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Siala, Wafi [UCL] Kucharíková, Soňa Braem, Annabel Vleugels, Jef Tulkens, Paul M. [UCL] Mingeot-Leclercq, Marie-Paule [UCL] Van Bambeke, Françoise [UCL]

Biofilms play a major role in Staphylococcus aureus pathogenicity but respond poorly to antibiotics. Here, we show that the antifungal caspofungin improves the activity of fluoroquinolones (moxifloxacin, delafloxacin) against S. aureus biofilms grown in vitro (96-well plates or catheters) and in vivo (murine model of implanted catheters). The degree of synergy among different clinical isolates is inversely proportional to the expression level of ica operon, the products of which synthesize poly-N-acetyl-glucosamine polymers, a major constituent of biofilm matrix. In vitro, caspofungin inhibits the activity of IcaA, which shares homology with β-1-3-glucan synthase (caspofungin's pharmacological target in fungi). This inhibition destructures the matrix, reduces the concentration and polymerization of exopolysaccharides in biofilms, and increases fluoroquinolone penetration inside biofilms. Our study identifies a bacterial target for caspofungin and indicates that IcaA inhibitors could potentially be useful in the treatment of biofilm-related infections.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 2016
Language Anglais
Journal information "Nature Communications" - Vol. 7, no.Nov.3, p. 13286 (2016)
Peer reviewed yes
Publisher Nature Publishing Group (London)
issn 2041-1723
e-issn 2041-1723
Publication status Publié
Affiliation UCL - SSS/LDRI - Louvain Drug Research Institute
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Bibliographic reference Siala, Wafi ; Kucharíková, Soňa ; Braem, Annabel ; Vleugels, Jef ; Tulkens, Paul M. ; et. al. The antifungal caspofungin increases fluoroquinolone activity against Staphylococcus aureus biofilms by inhibiting N-acetylglucosamine transferase. In: Nature Communications, Vol. 7, no.Nov.3, p. 13286 (2016)
Permanent URL http://hdl.handle.net/2078.1/177860