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EVI1-mediated down regulation of MIR449A is essential for the survival of EVI1 positive leukaemic cells

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De Weer, An Van der Meulen, Joni Rondou, Pieter Taghon, Tom Konrad, Torsten A. Poirel, Hélène [UCL] Speleman, Frank

Chromosomal rearrangements involving the MECOM (MDS1 and EVI1 complex) locus are recurrent genetic events in myeloid leukaemia and are associated with poor prognosis. In this study, we assessed the role of MECOM locus protein EVI1 in the transcriptional regulation of microRNAs (miRNAs) involved in the leukaemic phenotype. For this, we profiled expression of 366 miRNAs in 38 MECOM-rearranged patient samples, normal bone marrow controls and MECOM (EVI1) knock down/re-expression models. Cross-comparison of these miRNA expression profiling data showed that MECOM rearranged leukaemias are characterized by down regulation of MIR449A. Reconstitution of MIR449A expression in MECOM-rearranged cell line models induced apoptosis resulting in a strong decrease in cell viability. These effects might be mediated in part by MIR449A regulation of NOTCH1 and BCL2, which are shown here to be bona fide MIR449A targets. Finally, we confirmed that MIR449A repression is mediated through direct promoter occupation of the EVI1 transcriptional repressor. In conclusion, this study reveals MIR449A as a crucial direct target of the MECOM locus protein EVI1 involved in the pathogenesis of MECOM-rearranged leukaemias and unravels NOTCH1 and BCL2 as important novel targets of MIR449A. This EVI1-MIR449A-NOTCH1/BCL2 regulatory axis might open new possibilities for the development of therapeutic strategies in this poor prognostic leukaemia subgroup. © 2011 Blackwell Publishing Ltd.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2011
Language Anglais
Journal information "British Journal of Haematology" - Vol. 154, no. 3, p. 337-348 (2011)
Peer reviewed yes
Publisher Wiley-Blackwell Publishing Ltd. ((United Kingdom) Oxford)
issn 0007-1048
e-issn 1365-2141
Publication status Publié
Affiliations UCL - SSS/DDUV - Institut de Duve
UCL - (SLuc) Centre de génétique médicale UCL
Links
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Bibliographic reference De Weer, An ; Van der Meulen, Joni ; Rondou, Pieter ; Taghon, Tom ; Konrad, Torsten A. ; et. al. EVI1-mediated down regulation of MIR449A is essential for the survival of EVI1 positive leukaemic cells. In: British Journal of Haematology, Vol. 154, no. 3, p. 337-348 (2011)
Permanent URL http://hdl.handle.net/2078.1/163952