User menu

Activity-Fed Translation (AFT) Assay: A New High-Throughput Screening Strategy for Enzymes in Droplets.

Bibliographic reference Woronoff, Gabrielle ; Ryckelynck, Michaël ; Wessel, Julia ; Schicke, Olivier ; Griffiths, Andrew D ; et. al. Activity-Fed Translation (AFT) Assay: A New High-Throughput Screening Strategy for Enzymes in Droplets.. In: ChemBioChem : a European journal of chemical biology, Vol. 16, no. 9, p. 1343-1349 (2015)
Permanent URL
  1. Seixas de Melo J. Sergio, Becker Ralph S., Macanita Antonio L., Photophysical Behavior of Coumarins as a Function of Substitution and Solvent: Experimental Evidence for the Existence of a Lowest Lying 1(n,.pi.*) State, 10.1021/j100075a002
  2. Goddard Jean-Philippe, Reymond Jean-Louis, Recent advances in enzyme assays, 10.1016/j.tibtech.2004.04.005
  3. GODDARD J, REYMOND J, Enzyme assays for high-throughput screening, 10.1016/j.copbio.2004.06.008
  4. Wahler Denis, Reymond Jean-Louis, Novel methods for biocatalyst screening, 10.1016/s1367-5931(00)00184-8
  5. Henke Erik, Bornscheuer Uwe T., Schmid Rolf D., Pleiss Jürgen, A Molecular Mechanism of Enantiorecognition of Tertiary Alcohols by Carboxylesterases, 10.1002/cbic.200200518
  6. Konarzycka-Bessler Monika, Bornscheuer Uwe T., A High-Throughput-Screening Method for Determining the Synthetic Activity of Hydrolases, 10.1002/anie.200390365
  7. Konarzycka-Bessler Monika, Bornscheuer Uwe T., Eine Hochdurchsatz-Screeningmethode zur Bestimmung der Syntheseaktivität von Hydrolasen, 10.1002/ange.200390335
  8. Zhao, Methods in Molecular Biology, Vol. 230: Directed Enzyme Evolution: Screening and Selection Methods, 213 (2003)
  9. Theberge Ashleigh B., Courtois Fabienne, Schaerli Yolanda, Fischlechner Martin, Abell Chris, Hollfelder Florian, Huck Wilhelm T. S., Microdroplets in Microfluidics: An Evolving Platform for Discoveries in Chemistry and Biology, 10.1002/anie.200906653
  10. Theberge Ashleigh B., Courtois Fabienne, Schaerli Yolanda, Fischlechner Martin, Abell Chris, Hollfelder Florian, Huck Wilhelm T. S., Mikrotröpfchen in Mikrofluidiksystemen: eine Technik für Entdeckungen in der Chemie und Biologie, 10.1002/ange.200906653
  11. Devenish, Methods in Molecular Biology, Vol. 996: Protein Nanotechnology: Protocols, Instrumentation, and Applications,, 269 (2013)
  12. Agresti J. J., Antipov E., Abate A. R., Ahn K., Rowat A. C., Baret J.-C., Marquez M., Klibanov A. M., Griffiths A. D., Weitz D. A., Ultrahigh-throughput screening in drop-based microfluidics for directed evolution, 10.1073/pnas.0910781107
  13. Sjostrom Staffan L., Bai Yunpeng, Huang Mingtao, Liu Zihe, Nielsen Jens, Joensson Haakan N., Andersson Svahn Helene, High-throughput screening for industrial enzyme production hosts by droplet microfluidics, 10.1039/c3lc51202a
  14. Fallah-Araghi Ali, Baret Jean-Christophe, Ryckelynck Michael, Griffiths Andrew D., A completely in vitro ultrahigh-throughput droplet-based microfluidic screening system for protein engineering and directed evolution, 10.1039/c2lc21035e
  15. Shimizu Yoshihiro, Inoue Akio, Tomari Yukihide, Suzuki Tsutomu, Yokogawa Takashi, Nishikawa Kazuya, Ueda Takuya, 10.1038/90802
  16. Griffiths Andrew D, Tawfik Dan S, Man-made enzymes — from design to in vitro compartmentalisation, 10.1016/s0958-1669(00)00109-9
  17. Wang Qian, Parrish Angela R., Wang Lei, Expanding the Genetic Code for Biological Studies, 10.1016/j.chembiol.2009.03.001
  18. Woronoff Gabrielle, El Harrak Abdeslam, Mayot Estelle, Schicke Olivier, Miller Oliver J., Soumillion Patrice, Griffiths Andrew D., Ryckelynck Michael, New Generation of Amino Coumarin Methyl Sulfonate-Based Fluorogenic Substrates for Amidase Assays in Droplet-Based Microfluidic Applications, 10.1021/ac200373n
  19. Duggleby Helen J., Tolley Shirley P., Hill Christopher P., Dodson Eleanor J., Dodson Guy, Moody Peter C. E., Penicillin acylase has a single-amino-acid catalytic centre, 10.1038/373264a0
  20. Alkema Wynand B. L., Dijkhuis Anne-Jan, de Vries Erik, Janssen Dick B., The role of hydrophobic active-site residues in substrate specificity and acyl transfer activity of penicillin acylase : Role of active-site phenylalanines in penicillin acylase, 10.1046/j.1432-1033.2002.02857.x
  21. Stögbauer Tobias, Windhager Lukas, Zimmer Ralf, Rädler Joachim O., Experiment and mathematical modeling of gene expression dynamics in a cell-free system, 10.1039/c2ib00102k
  22. Sunami Takeshi, Hosoda Kazufumi, Suzuki Hiroaki, Matsuura Tomoaki, Yomo Tetsuya, Cellular Compartment Model for Exploring the Effect of the Lipidic Membrane on the Kinetics of Encapsulated Biochemical Reactions, 10.1021/la904569m
  23. Elbaz, Biochemica, 4, 23 (2004)
  24. Alkema Wynand B.L., Hensgens Charles M.H., Kroezinga Els H., de Vries Erik, Floris René, van der Laan Jan-Metske, Dijkstra Bauke W., Janssen Dick B., Characterization of the β-lactam binding site of penicillin acylase of Escherichia coli by structural and site-directed mutagenesis studies, 10.1093/protein/13.12.857
  25. Anna Shelley L., Bontoux Nathalie, Stone Howard A., Formation of dispersions using “flow focusing” in microchannels, 10.1063/1.1537519
  26. Savile C. K., Janey J. M., Mundorff E. C., Moore J. C., Tam S., Jarvis W. R., Colbeck J. C., Krebber A., Fleitz F. J., Brands J., Devine P. N., Huisman G. W., Hughes G. J., Biocatalytic Asymmetric Synthesis of Chiral Amines from Ketones Applied to Sitagliptin Manufacture, 10.1126/science.1188934
  27. Baret Jean-Christophe, Miller Oliver J., Taly Valerie, Ryckelynck Michaël, El-Harrak Abdeslam, Frenz Lucas, Rick Christian, Samuels Michael L., Hutchison J. Brian, Agresti Jeremy J., Link Darren R., Weitz David A., Griffiths Andrew D., Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity, 10.1039/b902504a
  28. Mastrobattista Enrico, Taly Valerie, Chanudet Estelle, Treacy Patrick, Kelly Bernard T., Griffiths Andrew D., High-Throughput Screening of Enzyme Libraries: In Vitro Evolution of a β-Galactosidase by Fluorescence-Activated Sorting of Double Emulsions, 10.1016/j.chembiol.2005.09.016
  29. Diamante L., Gatti-Lafranconi P., Schaerli Y., Hollfelder F., In vitro affinity screening of protein and peptide binders by megavalent bead surface display, 10.1093/protein/gzt039
  30. Dressman D., Yan H., Traverso G., Kinzler K. W., Vogelstein B., Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variations, 10.1073/pnas.1133470100
  31. Cohen H. M., Tawfik D. S., Griffiths A. D., Altering the sequence specificity of HaeIII methyltransferase by directed evolution using in vitro compartmentalization, 10.1093/protein/gzh001
  32. Wochner A., Attwater J., Coulson A., Holliger P., Ribozyme-Catalyzed Transcription of an Active Ribozyme, 10.1126/science.1200752
  33. Tawfik Dan S., Griffiths Andrew D., Man-made cell-like compartments for molecular evolution , 10.1038/nbt0798-652
  34. Ghadessy F. J., Ong J. L., Holliger P., Directed evolution of polymerase function by compartmentalized self-replication, 10.1073/pnas.071052198
  35. Courtois Fabienne, Olguin Luis F., Whyte Graeme, Bratton Daniel, Huck Wilhelm T. S., Abell Chris, Hollfelder Florian, An Integrated Device for Monitoring Time-Dependent in vitro Expression From Single Genes in Picolitre Droplets, 10.1002/cbic.200700536
  36. Holtze C., Rowat A. C., Agresti J. J., Hutchison J. B., Angilè F. E., Schmitz C. H. J., Köster S., Duan H., Humphry K. J., Scanga R. A., Johnson J. S., Pisignano D., Weitz D. A., Biocompatible surfactants for water-in-fluorocarbon emulsions, 10.1039/b806706f
  37. Pekin Deniz, Skhiri Yousr, Baret Jean-Christophe, Le Corre Delphine, Mazutis Linas, Ben Salem Chaouki, Millot Florian, El Harrak Abdeslam, Hutchison J. Brian, Larson Jonathan W., Link Darren R., Laurent-Puig Pierre, Griffiths Andrew D., Taly Valérie, Quantitative and sensitive detection of rare mutations using droplet-based microfluidics, 10.1039/c1lc20128j