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Variability of Mouse Left Ventricular Function Assessment by 11.7 Tesla MRI.

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Vanhoutte, Laetitia [UCL] Gallez, Bernard [UCL] Feron, Olivier [UCL] Balligand, Jean-Luc [UCL] Esfahani, Hrag [UCL] D'Hoore, William [UCL] Moniotte, Stéphane [UCL]

We studied intraobserver (n = 24), interobserver (n = 24) and interexperiment (n = 12) reproducibility of left ventricular (LV) mass and volume measurements in mice using an 11.7 T MRI system. The LV systolic function was assessed with a short-axis FLASH-cine sequence in 29 mice, including animals having undergone transverse aortic constriction. Bland-Altman and regression analysis were used to compare the different data sets. Reproducibility was excellent for the LV mass and end-diastolic volume (coefficient of variability (CoV) between 5.4 and 11.8 %), good for end-systolic volume (CoV 15.2-19.4 %) and moderate for stroke volume and ejection fraction (CoV 14.7-20.9 %). We found an excellent correlation between LV mass determined by MRI and ex vivo morphometric data (r = 0.92). In conclusion, LV systolic function can be assessed on an 11.7 T MRI scanner with high reproducibility for most parameters, as needed in longitudinal studies. However, data should be interpreted taking into account the moderate reproducibility of small volumes.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2015
Language Anglais
Journal information "Journal of Cardiovascular Translational Research" - Vol. 8, no. 6, p. 362-371 (2015)
Peer reviewed yes
Publisher Springer New York LLC ((United States) New York, NY)
issn 1937-5387
e-issn 1937-5395
Publication status Publié
Affiliations UCL - SSS/IREC/FATH - Pôle de Pharmacologie et thérapeutique
UCL - SSS/IREC/PEDI - Pôle de Pédiatrie
UCL - SSS/IRSS - Institut de recherche santé et société
UCL - SSS/LDRI - Louvain Drug Research Institute
UCL - (SLuc) Service de médecine interne et maladies infectieuses (MIMI)
UCL - (SLuc) Service de médecine nucléaire
UCL - (SLuc) Service de neurologie pédiatrique
UCL - (SLuc) Service de cardiologie pédiatrique
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Bibliographic reference Vanhoutte, Laetitia ; Gallez, Bernard ; Feron, Olivier ; Balligand, Jean-Luc ; Esfahani, Hrag ; et. al. Variability of Mouse Left Ventricular Function Assessment by 11.7 Tesla MRI.. In: Journal of Cardiovascular Translational Research, Vol. 8, no. 6, p. 362-371 (2015)
Permanent URL http://hdl.handle.net/2078.1/161088