User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

Alternative electron transports participate in the maintenance of violaxanthin de-epoxidase activity of Ulva sp. under low irradiance

Primary tabs

Xie, Xiujun [ianjin Key Laboratory of Marine Resources and Chemistry, College of Marine Science and Engineering, Tianjin University of Science and Technology, Tianjin, China] Gu, Wenhui [Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China] Gao, Shan [Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China] Lu, Shan [School of Life Science, Nanjing University, Nanjing, China] Li, Jian [UCL] Shen, Songdong [College of Life Sciences, Soochow University, Suzhou, China]

The xanthophyll cycle (Xc), which involves violaxanthin de-epoxidase (VDE) and the zeaxanthin epoxidase (ZEP), is one of the most rapid and efficient responses of plant and algae to high irradiance. High light intensity can activate VDE to convert violaxanthin (Vx) to zeaxanthin (Zx) via antheraxanthin (Ax). However, it remains unclear whether VDE remains active under low light or dark conditions when there is no significant accumulation of Ax and Zx, and if so, how the ΔpH required for activation of VDE is built. In this study, we used salicylaldoxime (SA) to inhibit ZEP activity in the intertidal macro-algae Ulva sp. (Ulvales, Chlorophyta) and then characterized VDE under low light and dark conditions with various metabolic inhibitors. With inhibition of ZEP by SA, VDE remained active under low light and dark conditions, as indicated by large accumulations of Ax and Zx at the expense of Vx. When PSII-mediated linear electron transport systems were completely inhibited by SA and DCMU, alternative electron transport systems (i.e., cyclic electron transport and chlororespiration) could maintain VDE activity. Furthermore, accumulations of Ax and Zx decreased significantly when SA, DCMU, or DBMIB together with an inhibitor of chlororespiration (i.e., propyl gallate (PG)) were applied to Ulva sp. This result suggests that chlororespiration not only participates in the build-up of the necessary DpH, but that it also possibly influences VDE activity indirectly by diminishing the oxygen level in the chloroplast. © 2013 Xie et al.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 2013
Language Anglais
Journal information "PLoS One" - Vol. 8, no. 11 (2013)
Peer reviewed yes
issn 1932-6203
e-issn 1932-6203
Publication status Publié
Affiliation UCL - SST/ELI - Earth and Life Institute
Links
  1. Demmig-Adams B, Adams W W, Photoprotection and Other Responses of Plants to High Light Stress, 10.1146/annurev.pp.43.060192.003123
  2. Niyogi Krishna K., PHOTOPROTECTION REVISITED:Genetic and Molecular Approaches, 10.1146/annurev.arplant.50.1.333
  3. Yamamoto H, Bugos R, David Hieber A (1999) Biochemistry and molecular biology of the xanthophyll cycle. In: Frank HA, Young AJ, Britton G, Cogdell RJ, editors. The Photochemistry of Carotenoids. The Netherlands: Kluwer Academic Publisher. pp. 293–303.
  4. Goss Reimund, Jakob Torsten, Regulation and function of xanthophyll cycle-dependent photoprotection in algae, 10.1007/s11120-010-9536-x
  5. Ruban Alexander V., Johnson Matthew P., Duffy Christopher D.P., The photoprotective molecular switch in the photosystem II antenna, 10.1016/j.bbabio.2011.04.007
  6. Arvidsson Per-Ola, Bratt Charlotte Eva, Carlsson Marie, �kerlund Hans-Erik, Purification and identification of the violaxanthin deepoxidase as a 43 kDa protein, 10.1007/bf00117662
  7. Rockholm D. C., Yamamoto H. Y., Violaxanthin De-Epoxidase (Purification of a 43-Kilodalton Lumenal Protein from Lettuce by Lipid-Affinity Precipitation with Monogalactosyldiacylglyceride), 10.1104/pp.110.2.697
  8. E Marin, The EMBO Journal, 15, 2331 (1996)
  9. Hurry V., Anderson J. M., Chow W. S., Osmond C. B., Accumulation of Zeaxanthin in Abscisic Acid-Deficient Mutants of Arabidopsis Does Not Affect Chlorophyll Fluorescence Quenching or Sensitivity to Photoinhibition in Vivo, 10.1104/pp.113.2.639
  10. Niyogi K. K., Arabidopsis Mutants Define a Central Role for the Xanthophyll Cycle in the Regulation of Photosynthetic Energy Conversion, 10.1105/tpc.10.7.1121
  11. Niyogi K. K., Bjorkman O., Grossman A. R., The roles of specific xanthophylls in photoprotection, 10.1073/pnas.94.25.14162
  12. Xu Chang Cheng, Jeon Young Ah, Hwang Hong Jin, Lee Choon-Hwan, Suppression of zeaxanthin epoxidation by chloroplast phosphatase inhibitors in rice leaves, 10.1016/s0168-9452(99)00084-9
  13. Berg Steven P., Izawa S., Concentration-dependent effects of salicylaldoxime on chloroplast reactions, 10.1016/0005-2728(76)90036-0
  14. Yamamoto Harry Y., Kamite Lavonne, The effects of dithiothreitol on violaxanthin de-epoxidation and absorbance changes in the 500-nm region, 10.1016/0005-2728(72)90182-x
  15. Karlish S. J. D., Shavit N., Avron M., On the Mechanism of Uncoupling in Chloroplasts by Ion-Permeability Inducing Agents, 10.1111/j.1432-1033.1969.tb00608.x
  16. Kleczkowski L A, Inhibitors of Photosynthetic Enzymes/Carriers and Metabolism, 10.1146/annurev.pp.45.060194.002011
  17. Cruz J. A., Salbilla B. A., Kanazawa A., Kramer D. M., Inhibition of Plastocyanin to P700+ Electron Transfer in Chlamydomonas reinhardtii by Hyperosmotic Stress, 10.1104/pp.010328
  18. Cournac L., Josse E.-M., Joet T., Rumeau D., Redding K., Kuntz M., Peltier G., Flexibility in photosynthetic electron transport: a newly identified chloroplast oxidase involved in chlororespiration, 10.1098/rstb.2000.0705
  19. Thayer Susan S., Bj�rkman Olle, Leaf Xanthophyll content and composition in sun and shade determined by HPLC, 10.1007/bf00034864
  20. K Maxwell, J Exp Bot, 51, 659 (2000)
  21. A Hager, Ber Deutsch Bot Ges, 79, 94 (1966)
  22. Golbeck John H., Warden Joseph T., Site of salicylaldoxime interaction with photosystem II, 10.1007/bf00054110
  23. Havir Evelyn A., Tausta S.Lorraine, Peterson Richard B., Purification and properties of violaxanthin de-epoxidase from spinach, 10.1016/s0168-9452(97)04579-2
  24. Bennoun P., Evidence for a respiratory chain in the chloroplast, 10.1073/pnas.79.14.4352
  25. Peltier Gilles, Cournac Laurent, CHLORORESPIRATION, 10.1146/annurev.arplant.53.100301.135242
  26. Fernández-Marín Beatriz, Míguez Fátima, Becerril José, García-Plazaola José, Activation of violaxanthin cycle in darkness is a common response to different abiotic stresses: a case study in Pelvetia canaliculata, 10.1186/1471-2229-11-181
  27. Barr R., Crane F. L., Organization of Electron Transport in Photosystem II of Spinach Chloroplasts According to Chelator Inhibition Sites, 10.1104/pp.57.3.450
  28. Golbeck John H., Action of salicylaldoxime on electron transport reactions, fluorescence yield, and light-induced field changes in spinach chloroplasts, 10.1016/0003-9861(80)90450-6
  29. Takahashi Hideyuki, Watanabe Ayako, Tanaka Ayumi, Hashida Shin-nosuke, Kawai-Yamada Maki, Sonoike Kintake, Uchimiya Hirofumi, Chloroplast NAD Kinase is Essential for Energy Transduction Through the Xanthophyll Cycle in Photosynthesis, 10.1093/pcp/pcl029
  30. Gruszecki Wiesŀaw I., Strzaŀka Kazimierz, Does the xanthophyll cycle take part in the regulation of fluidity of the thylakoid membrane?, 10.1016/s0005-2728(05)80322-6
  31. G Thomas, Plant Mol Biol, 50, 129 (2002)
  32. Havaux M., Dall'Osto L., Bassi R., Zeaxanthin Has Enhanced Antioxidant Capacity with Respect to All Other Xanthophylls in Arabidopsis Leaves and Functions Independent of Binding to PSII Antennae, 10.1104/pp.107.108480
  33. Johnson Matthew P., Havaux Michel, Triantaphylidès Christian, Ksas Brigitte, Pascal Andrew A., Robert Bruno, Davison Paul A., Ruban Alexander V., Horton Peter, Elevated Zeaxanthin Bound to Oligomeric LHCII Enhances the Resistance ofArabidopsisto Photooxidative Stress by a Lipid-protective, Antioxidant Mechanism, 10.1074/jbc.m702831200
  34. Takeguchi C.A., Yamamoto H.Y., Light-induced 18O2 uptake by epoxy xanthophylls in New Zealand spinach leaves (Tetragonia expansa), 10.1016/0005-2728(68)90087-x
  35. Büch Karen, Stransky Harald, Hager Achim, FAD is a further essential cofactor of the NAD(P)H and O2-dependent zeaxanthin-epoxidase, 10.1016/0014-5793(95)01243-9
  36. Bratt Charlotte Eva, Arvidsson Per-Ola, Carlsson Marie, �kerlund Hans-Erik, Regulation of violaxanthin de-epoxidase activity by pH and ascorbate concentration, 10.1007/bf00032588
  37. Holtgrefe S., Decreased Content of Leaf Ferredoxin Changes Electron Distribution and Limits Photosynthesis in Transgenic Potato Plants, 10.1104/pp.103.026013
  38. Bernhard Teicher Harald, Vibe Scheller Henrik, The NAD(P)H Dehydrogenase in Barley Thylakoids Is Photoactivatable and Uses NADPH as well as NADH, 10.1104/pp.117.2.525
  39. Nixon P. J., Chlororespiration, 10.1098/rstb.2000.0714
  40. Friedrich Thorsten, Steinmüller Klaus, Weiss Hanns, The proton-pumping respiratory complex I of bacteria and mitochondria and its homologue in chloroplasts, 10.1016/0014-5793(95)00548-n
  41. Cournac Laurent, Redding Kevin, Ravenel Jacques, Rumeau Dominique, Josse Eve-Marie, Kuntz Marcel, Peltier Gilles, Electron Flow between Photosystem II and Oxygen in Chloroplasts of Photosystem I-deficient Algae Is Mediated by a Quinol Oxidase Involved in Chlororespiration, 10.1074/jbc.m908732199
  42. Mittler Ron, Oxidative stress, antioxidants and stress tolerance, 10.1016/s1360-1385(02)02312-9
  43. Rizhsky Ludmila, Hallak-Herr Elza, Van Breusegem Frank, Rachmilevitch Shimon, Barr Jason E., Rodermel Steven, Inzé Dirk, Mittler Ron, Double antisense plants lacking ascorbate peroxidase and catalase are less sensitive to oxidative stress than single antisense plants lacking ascorbate peroxidase or catalase, 10.1046/j.1365-313x.2002.01427.x
  44. McDonald Allison E., Ivanov Alex G., Bode Rainer, Maxwell Denis P., Rodermel Steven R., Hüner Norman P.A., Flexibility in photosynthetic electron transport: The physiological role of plastoquinol terminal oxidase (PTOX), 10.1016/j.bbabio.2010.10.024
  45. Asada K., The water-water cycle as alternative photon and electron sinks, 10.1098/rstb.2000.0703
  46. Cleland Robyn E., Grace Stephen C., Voltammetric detection of superoxide production by photosystem II, 10.1016/s0014-5793(99)01067-4
  47. Triantaphylidès Christian, Havaux Michel, Singlet oxygen in plants: production, detoxification and signaling, 10.1016/j.tplants.2009.01.008
  48. Noctor Graham, Foyer Christine H., ASCORBATE AND GLUTATHIONE: Keeping Active Oxygen Under Control, 10.1146/annurev.arplant.49.1.249
  49. Muller-Moule P., Conklin P. L., Niyogi K. K., Ascorbate Deficiency Can Limit Violaxanthin De-Epoxidase Activity in Vivo, 10.1104/pp.010924
Bibliographic reference Xie, Xiujun ; Gu, Wenhui ; Gao, Shan ; Lu, Shan ; Li, Jian ; et. al. Alternative electron transports participate in the maintenance of violaxanthin de-epoxidase activity of Ulva sp. under low irradiance. In: PLoS One, Vol. 8, no. 11 (2013)
Permanent URL http://hdl.handle.net/2078.1/160762