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Cryopreservation of arbuscular mycorrhizal fungi from root organ and plant cultures

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Lalaymia, Ismahen [UCL] Cranenbrouck, Sylvie [UCL] Naveau, Françoise [UCL] Declerck, Stephan [UCL]

Long-term maintenance of arbuscular mycorrhizal fungi (AMF) by in vitro or in vivo subcultivation is often expensive and time-consuming and could present the risk of contaminations and possibly morphological, physiological, and genetic variations over time. Recently, in vitro produced AMF isolates belonging to the genus Rhizophagus were successfully cryopreserved at -130 °C following encapsulation-drying. Here, this method was tested on 12 single species cultures belonging to six different genera (i.e., Rhizophagus, Glomus, Claroideoglomus, Septoglomus, Paraglomus, and Gigaspora) produced in vitro or in vivo. Their viability was estimated, after 1 month of cryopreservation at -130 °C, by the percentage of potentially infective beads (i.e., the percentage of beads that contained at least one germinated propagule) for the in vitro produced species and the percentage of infective beads (i.e., the percentage of beads that contained at least one propagule able to colonize a new host plant in pot culture) for the in vivo produced species. With the exception of Gigaspora sp. MUCL 52331 and Septoglomus constrictus PER 7.2, no significant differences were observed in the viability of the single species cultures before and after cryopreservation. These results, thus, demonstrated the suitability of the cryopreservation method by encapsulation-drying for AMF species belonging to different genera and produced in vitro or in vivo. This method opens the door to the long-term preservation at ultra-low temperature of a large number of AMF species and for the preservation of species that are still recalcitrant to in vitro cultivation. © 2013 Springer-Verlag Berlin Heidelberg.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2014
Language Anglais
Journal information "Mycorrhiza" - Vol. 24, no. 3, p. 233-237 (2014)
Peer reviewed yes
Publisher Springer
e-issn 1432-1890
issn 0940-6360
Publication status Publié
Affiliation UCL - SST/ELI/ELIM - Applied Microbiology
Keywords Arbuscular mycorrhizal fungi ; Glomeromycota ; Continuous culture ; Cryopreservation ; Encapsulation-drying ; 3130
Links
  1. Dalpé Yolande, Cranenbrouck Sylvie, Séguin Sylvie, Declerck Stéphane, The Monoxenic Culture of Arbuscular Mycorrhizal Fungi as a Tool for Systematics and Biodiversity, Soil Biology ISBN:3540240276 p.31-48, 10.1007/3-540-27331-x_3
  2. de Souza Francisco Adriano, Declerck Stephane, Mycelium Development and Architecture, and Spore Production of Scutellospora reticulata in Monoxenic Culture with Ri T-DNA Transformed Carrot Roots, 10.2307/3761908
  3. Declerck S, Strullu D.G, Plenchette C, Guillemette T, Entrapment of in vitro produced spores of Glomus versiforme in alginate beads: in vitro and in vivo inoculum potentials, 10.1016/0168-1656(96)01396-x
  4. Declerck Stephane, Strullu Desire G., Plenchette Christian, Monoxenic Culture of the Intraradical Forms of Glomus sp. Isolated from a Tropical Ecosystem: A Proposed Methodology for Germplasm Collection, 10.2307/3761216
  5. DECLERCK S., ANGELO-VAN COPPENOLLE M. G., Cryopreservation of entrapped monoxenically produced spores of an arbuscular mycorrhizal fungus : RESEARCH Cryopreservation of entrapped monoxenic AMF spores, 10.1046/j.1469-8137.2000.00740.x
  6. S. Declerck, D. D'or, S. Cranenbrouck, Boulengé Le, Modelling the sporulation dynamics of arbuscular mycorrhizal fungi in monoxenic culture, 10.1007/s005720100124
  7. Declerck Stéphane, D'Or Dimitri, Bivort Céline, de Souza Francisco A., Development of extraradical mycelium of Scutellospora reticulata under root-organ culture: spore production and function of auxiliary cells, 10.1017/s0953756203008761
  8. DOUDS DAVID D., SCHENCK N. C., Cryopreservation of spores of vesicular-arbuscular mycorrhizal fungi*, 10.1111/j.1469-8137.1990.tb00498.x
  9. Karandashov VE, Kuzourina IN, George E, Marschner H (1999) Monoxenic culture of arbuscular fungi and plant hairy roots. Russ J Plant Physiol 46:87–92
  10. Krüger Manuela, Krüger Claudia, Walker Christopher, Stockinger Herbert, Schüßler Arthur, Phylogenetic reference data for systematics and phylotaxonomy of arbuscular mycorrhizal fungi from phylum to species level, 10.1111/j.1469-8137.2011.03962.x
  11. Kuszala C, Gianinazzi S, Gianinazzi-Pearson V (2001) Storage conditions for the long-term survival of AM fungal propagules in wet sieved soil fractions. Symbiosis 30:287–299
  12. Lalaymia Ismahen, Cranenbrouck Sylvie, Draye Xavier, Declerck Stéphane, Preservation at ultra-low temperature of in vitro cultured arbuscular mycorrhizal fungi via encapsulation–drying, 10.1016/j.funbio.2012.07.007
  13. Lalaymia I, Declerck S, Cranenbrouck S (2013) Cryopreservation of in vitro-produced Rhizophagus species has minor effects on their morphology, physiology, and genetic stability. Mycorrhiza. doi: 10.1007/s00572-013-0506-y
  14. Plenchette C., Declerck S., Diop T. A., Strullu D. G., Infectivity of monoaxenic subcultures of the arbuscular mycorrhizal fungus Glomus versiforme associated with Ri-T-DNA-transformed carrot root, 10.1007/s002530050858
  15. Schüßler A, Walker C (2010) The Glomeromycota. A species list with new families and new genera. Published in Libraries at the Royal Botanic Garden Edinburgh, the Royal Botanic Garden Kew, Botanische Staatssammlung Munich, and Oregon State University.
  16. Walker C (2005) A simple blue staining technique for arbuscular mycorrhizal and other root-inhabiting fungi. Inoculum 56:68–69
  17. Young J. Peter W., A molecular guide to the taxonomy of arbuscular mycorrhizal fungi : Commentary, 10.1111/j.1469-8137.2011.04029.x
Bibliographic reference Lalaymia, Ismahen ; Cranenbrouck, Sylvie ; Naveau, Françoise ; Declerck, Stephan. Cryopreservation of arbuscular mycorrhizal fungi from root organ and plant cultures. In: Mycorrhiza, Vol. 24, no. 3, p. 233-237 (2014)
Permanent URL http://hdl.handle.net/2078.1/159718