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Fibrin hydrogels as Stem Cells of the Apical Papilla (SCAP) Scaffold for regenerative medicine

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Germain, Loïc [UCL] De Berdt, Pauline [UCL] Vanacker, Julie [UCL] Leprince, Julian [UCL] Diogenes, Anibal Jacobs, Damien [UCL] Vandermeulen, Gaëlle [UCL] Bouzin, Caroline [UCL] Préat, Véronique [UCL] Dupont-Gillain, Christine C. [UCL] des Rieux, Anne [UCL]

Evaluation of survival, proliferation and neurodifferentiation of dental stem cells from the apical papilla (SCAP) in fibrin hydrogels. We hypothesized that fibrin composition will influence cell behavior. Methods: Modulus, pore and fiber size were measured. SCAP in vitro viability, proliferation and neural differentiation, as well as in vivo proliferation and angiogenesis were studied. Results: Hydrogel moduli were influenced by fibrin formulation but not hydrogel morphology, SCAP in vitro viability and proliferation. In total 60% of SCAP expressed PanNeurofilament in vitro without induction in Fibrinogen50-Thrombin10. SCAP proliferated when implanted in vivo and stimulated host endothelial cell infiltration. Conclusion: Fibrinogen30-Thrombin10 or Thrombin50 would be more favorable to in vitro SCAP viability and in vivo proliferation, while Fibrinogen50-Thrombin50 would be more adapted to neurodifferentiation

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2015
Language Anglais
Journal information "Regenerative Medicine" - Vol. 10, no. 2, p. 153-167 (2015)
Peer reviewed yes
Publisher Future Medicine Ltd. ((United Kingdom) London)
issn 1746-0751
e-issn 1746-076X
Publication status Publié
Affiliations UCL - SSS/LDRI - Louvain Drug Research Institute
UCL - SST/IMCN/BSMA - Bio and soft matter
UCL - (SLuc) Service de dentisterie conservatrice et d'endodontie
Keywords angiogenesis ; dental stem cells ; fibrin ; neurodifferentiation ; tissue engineering
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Bibliographic reference Germain, Loïc ; De Berdt, Pauline ; Vanacker, Julie ; Leprince, Julian ; Diogenes, Anibal ; et. al. Fibrin hydrogels as Stem Cells of the Apical Papilla (SCAP) Scaffold for regenerative medicine. In: Regenerative Medicine, Vol. 10, no. 2, p. 153-167 (2015)
Permanent URL http://hdl.handle.net/2078.1/157261