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Callose and cellulose synthase gene expression analysis from the tight cluster to the full bloom stage and during early fruit development in Malus × domestica

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Guerriero, Gea [Laimburg Research Centre for Agriculture and Forestry, Auer (Italy)] Giorno, Filimona [Laimburg Research Centre for Agriculture and Forestry, Auer (Italy)] Folgado, Raquel [Laboratory of Tropical Crop improvment, KUleuven] Printz, Bruno [UCL] Baric, Sanja [Laimburg Research Centre for Agriculture and Forestry, Auer (Italy)] Hausman, Jean-françois [Department Environment and Agro-biotechnologies, Centre de recherche Public Gabriel Lippman, Luxembourg]

Apple (Malus × domestica) is an economically important temperate fruit-bearing crop which belongs to the family of Rosaceae and its pomaceous fruit is one of the most commonly cultivated. Several studies have demonstrated that the cell wall plays a pivotal role during flower and fruit development. It takes active part in pollen tube growth and contributes to determine the fruit firmness trait through the action of cell wall-related enzymes (i.e. polygalacturonase and pectinmethylesterase). We have investigated the expression of callose and cellulose synthase genes during flowering from tight cluster to anthesis and during early fruit development in domesticated apple. We also link the changes observed in gene expression to the profile of soluble non-structural carbohydrates at different developmental stages of flowers/fruitlets and to the qualitative results linked to wall polysaccharides' composition obtained through near-infrared spectroscopy. This work represents an important addition to the study of tree physiology with respect to the analysis of the expression of callose and cellulose synthase genes during flower and early fruit development in domesticated apple. © 2013 The Botanical Society of Japan and Springer Japan.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès interdit
Publication date 2014
Language Anglais
Journal information "Journal of Plant Research" - Vol. 127, no.1, p. 173-183 (2014)
Peer reviewed yes
Publisher Springer Japan KK ((Japan) Tokyo)
issn 0918-9440
e-issn 1618-0860
Publication status Publié
Affiliation UCL - SST/ELI/ELIA - Agronomy
Keywords Callose synthase ; Cell wall ; Cellulose synthase ; Flower and fruit development ; Malus × domestica ; Malus ; Malus x domestica ; Rosaceae
Links
  1. Abercrombie Jason M, O'Meara Brian C, Moffatt Andrew R, Williams Joseph H, Developmental evolution of flowering plant pollen tube cell walls: callose synthase (CalS) gene expression patterns, 10.1186/2041-9139-2-14
  2. Ali M, Emsley A.M, Herman H, Heywood R.J, Spectroscopic studies of the ageing of cellulosic paper, 10.1016/s0032-3861(00)00691-1
  3. Atkinson R. G., Gardner R. C., A Polygalacturonase Gene from Kiwifruit (Actinidia deliciosa), 10.1104/pp.103.2.669
  4. Atkinson R. G., Overexpression of Polygalacturonase in Transgenic Apple Trees Leads to a Range of Novel Phenotypes Involving Changes in Cell Adhesion, 10.1104/pp.010986
  5. Barratt D. H. P., Kolling K., Graf A., Pike M., Calder G., Findlay K., Zeeman S. C., Smith A. M., Callose Synthase GSL7 Is Necessary for Normal Phloem Transport and Inflorescence Growth in Arabidopsis, 10.1104/pp.110.166330
  6. Baum Andreas, Agger Jane, Meyer Anne S., Egebo Max, Mikkelsen Jørn Dalgaard, Rapid near infrared spectroscopy for prediction of enzymatic hydrolysis of corn bran after various pretreatments, 10.1016/j.nbt.2011.11.010
  7. Bieleski R. L., Fructan Hydrolysis Drives Petal Expansion in the Ephemeral Daylily Flower, 10.1104/pp.103.1.213
  8. Boyer J, Liu RH (2004) Apple phytochemicals and their health benefits. Nutr J 12:3–5
  9. Brummell David A., Harpster Mark H., 10.1023/a:1010656104304
  10. Brummell David A., Harpster Mark H., Dunsmuir Pamela, 10.1023/a:1006130018931
  11. Budzinski I. G. F., Santos T. B., Sera T., Pot D., Vieira L. G. E., Pereira L. F. P., Expression patterns of three α-expansin isoforms in Coffea arabica during fruit development : Coffea expansins, 10.1111/j.1438-8677.2010.00400.x
  12. Bustin S. A., Benes V., Garson J. A., Hellemans J., Huggett J., Kubista M., Mueller R., Nolan T., Pfaffl M. W., Shipley G. L., Vandesompele J., Wittwer C. T., The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments, 10.1373/clinchem.2008.112797
  13. Cai G., Faleri C., Del Casino C., Emons A. M. C., Cresti M., Distribution of Callose Synthase, Cellulose Synthase, and Sucrose Synthase in Tobacco Pollen Tube Is Controlled in Dissimilar Ways by Actin Filaments and Microtubules, 10.1104/pp.110.171371
  14. Callahan A. M., Morgens P. H., Wright P., Nichols K. E., Comparison of Pch313 (pTOM13 Homolog) RNA Accumulation during Fruit Softening and Wounding of Two Phenotypically Different Peach Cultivars, 10.1104/pp.100.1.482
  15. Cantu D., Vicente A. R., Greve L. C., Dewey F. M., Bennett A. B., Labavitch J. M., Powell A. L. T., The intersection between cell wall disassembly, ripening, and fruit susceptibility to Botrytis cinerea, 10.1073/pnas.0709813105
  16. Cantu Dario, Vicente Ariel R., Labavitch John M., Bennett Alan B., Powell Ann L.T., Strangers in the matrix: plant cell walls and pathogen susceptibility, 10.1016/j.tplants.2008.09.002
  17. Carroll A., Mansoori N., Li S., Lei L., Vernhettes S., Visser R. G. F., Somerville C., Gu Y., Trindade L. M., Complexes with Mixed Primary and Secondary Cellulose Synthases Are Functional in Arabidopsis Plants, 10.1104/pp.112.199208
  18. Chaib J., Devaux M.-F., Grotte M.-G., Robini K., Causse M., Lahaye M., Marty I., Physiological relationships among physical, sensory, and morphological attributes of texture in tomato fruits, 10.1093/jxb/erm046
  19. Civello Pedro Marcos, Powell Ann L.T., Sabehat Adnan, Bennett Alan B., An Expansin Gene Expressed in Ripening Strawberry Fruit, 10.1104/pp.121.4.1273
  20. Cosgrove Daniel J., Growth of the plant cell wall, 10.1038/nrm1746
  21. Costa Fabrizio, Alba Rob, Schouten Henk, Soglio Valeria, Gianfranceschi Luca, Serra Sara, Musacchi Stefano, Sansavini Silviero, Costa Guglielmo, Fei Zhangjun, Giovannoni James, Use of homologous and heterologous gene expression profiling tools to characterize transcription dynamics during apple fruit maturation and ripening, 10.1186/1471-2229-10-229
  22. Devoghalaere Fanny, Doucen Thomas, Guitton Baptiste, Keeling Jeannette, Payne Wendy, Ling Toby, Ross John, Hallett Ian, Gunaseelan Kularajathevan, Dayatilake GA, Diak Robert, Breen Ken C, Tustin D Stuart, Costes Evelyne, Chagné David, Schaffer Robert, David Karine, A genomics approach to understanding the role of auxin in apple (Malus x domestica) fruit size control, 10.1186/1471-2229-12-7
  23. Dong Y-H, Janssen B-J, Bieleski LRF, Atkinson RG, Morris BAM (1997) Isolating and characterizing genes differentially expressed early in apple fruit development. J Am Soc Hortic Sci 122:752–757
  24. Fujimoto T, Kurata Y, Matsumoto K, Tsuchika S (2008) Application of near infrared spectroscopy for estimating wood mechanical properties of small clear and full length lumber specimens. JNIRS 16:529–537
  25. Gallus S., Does an apple a day keep the oncologist away?, 10.1093/annonc/mdi361
  26. Gambino Giorgio, Perrone Irene, Gribaudo Ivana, A Rapid and effective method for RNA extraction from different tissues of grapevine and other woody plants, 10.1002/pca.1078
  27. Geisler-Lee J., Geisler M., Coutinho P. M., Segerman B., Nishikubo N., Takahashi J., Aspeborg H., Djerbi S., Master E., Andersson-Gunneras S., Sundberg B., Karpinski S., Teeri T. T., Kleczkowski L. A., Henrissat B., Mellerowicz E. J., Poplar Carbohydrate-Active Enzymes. Gene Identification and Expression Analyses, 10.1104/pp.105.072652
  28. Giorno Filomena, Guerriero Gea, Baric Sanja, Mariani Celestina, Heat shock transcriptional factors in Malus domestica: identification, classification and expression analysis, 10.1186/1471-2164-13-639
  29. Gu Z., Cavalcanti A., Chen F.-C., Bouman P., Li W.-H., Extent of Gene Duplication in the Genomes of Drosophila, Nematode, and Yeast, 10.1093/oxfordjournals.molbev.a004079
  30. Guerriero Gea, Spadiut Oliver, Kerschbamer Christine, Giorno Filomena, Baric Sanja, Ezcurra Inés, Analysis of cellulose synthase genes from domesticated apple identifies collinear genes WDR53 and CesA8A: partial co-expression, bicistronic mRNA, and alternative splicing of CESA8A, 10.1093/jxb/ers255
  31. Guerriero G., Giorno F., Ciccotti A. M., Schmidt S., Baric S., A gene expression analysis of cell wall biosynthetic genes in Malus x domestica infected by 'Candidatus Phytoplasma mali', 10.1093/treephys/tps095
  32. Guignard Cédric, Jouve Laurent, Bogéat-Triboulot Marie Béatrice, Dreyer Erwin, Hausman Jean-François, Hoffmann Lucien, Analysis of carbohydrates in plants by high-performance anion-exchange chromatography coupled with electrospray mass spectrometry, 10.1016/j.chroma.2005.05.068
  33. Guo Shaogui, Liu Jingan, Zheng Yi, Huang Mingyun, Zhang Haiying, Gong Guoyi, He Hongju, Ren Yi, Zhong Silin, Fei Zhangjun, Xu Yong, Characterization of transcriptome dynamics during watermelon fruit development: sequencing, assembly, annotation and gene expression profiles, 10.1186/1471-2164-12-454
  34. Hadfield Kristen A., Rose Jocelyn K.C., Yaver Debbie S., Berka Randy M., Bennett Alan B., Polygalacturonase Gene Expression in Ripe Melon Fruit Supports a Role for Polygalacturonase in Ripening-Associated Pectin Disassembly, 10.1104/pp.117.2.363
  35. Hamann Thorsten, Denness Lucy, Cell wall integrity maintenance in plants: Lessons to be learned from yeast?, 10.4161/psb.6.11.17782
  36. Hames Bonnie R., Thomas Steven R., Sluiter Amie D., Roth Christine J., Templeton David W., Rapid Biomass Analysis: New Tools for Compositional Analysis of Corn Stover Feedstocks and Process Intermediates from Ethanol Production, 10.1385/abab:105:1-3:5
  37. Hanke M-V, Flachowsky H, Peil A, Hättasch C (2007) No flower no fruit—genetic potentials to trigger flowering in fruit trees. Genes Genomes Genomics 1:1–20
  38. Hématy Kian, Cherk Candice, Somerville Shauna, Host–pathogen warfare at the plant cell wall, 10.1016/j.pbi.2009.06.007
  39. Hiwasa Kyoko, Rose Jocelyn K. C., Nakano Ryohei, Inaba Akitsugu, Kubo Yasutaka, Differential expression of seven alpha-expansin genes during growth and ripening of pear fruit, 10.1034/j.1399-3054.2003.00064.x
  40. Hong Z., A Cell Plate-Specific Callose Synthase and Its Interaction with Phragmoplastin, 10.1105/tpc.13.4.755
  41. Huang Lijun, Chen Xiong-Yan, Rim Yeonggil, Han Xiao, Cho Won Kyong, Kim Seon-Won, Kim Jae-Yean, Arabidopsis glucan synthase-like 10 functions in male gametogenesis, 10.1016/j.jplph.2008.06.010
  42. Humphrey Tania V., Bonetta Dario T., Goring Daphne R., Sentinels at the wall: cell wall receptors and sensors, 10.1111/j.1469-8137.2007.02192.x
  43. Ishimaru Megumi, Smith David L., Gross Kenneth C., Kobayashi Shozo, Expression of three expansin genes during development and maturation of Kyoho grape berries, 10.1016/j.jplph.2006.07.017
  44. Jackson John E., The Biology of Apples and Pears, ISBN:9780511542657, 10.1017/cbo9780511542657
  45. Janssen Bart J, Thodey Kate, Schaffer Robert J, Alba Rob, Balakrishnan Lena, Bishop Rebecca, Bowen Judith H, Crowhurst Ross N, Gleave Andrew P, Ledger Susan, McArtney Steve, Pichler Franz B, Snowden Kimberley C, Ward Shayna, Global gene expression analysis of apple fruit development from the floral bud to ripe fruit, 10.1186/1471-2229-8-16
  46. Jin Y., Ni D.-A., Ruan Y.-L., Posttranslational Elevation of Cell Wall Invertase Activity by Silencing Its Inhibitor in Tomato Delays Leaf Senescence and Increases Seed Weight and Fruit Hexose Level, 10.1105/tpc.108.063719
  47. Kim Y. K., Xiao C. L., A Postharvest Fruit Rot in Apple Caused by Phacidiopycnis washingtonensis, 10.1094/pd-90-1376
  48. Kutsunai S. Y., Lin A. C., Percival F. W., Laties G. G., Christoffersen R. E., Ripening-Related Polygalacturonase cDNA from Avocado, 10.1104/pp.103.1.289
  49. Lebon G., Wojnarowiez G., Holzapfel B., Fontaine F., Vaillant-Gaveau N., Clement C., Sugars and flowering in the grapevine (Vitis vinifera L.), 10.1093/jxb/ern135
  50. Lee Young-Pyo, Yu Gyung-Hee, Seo Young Sam, Han Sang Eun, Choi Yeon-Ok, Kim Daeil, Mok Il-Gin, Kim Woo Taek, Sung Soon-Kee, Microarray analysis of apple gene expression engaged in early fruit development, 10.1007/s00299-007-0308-9
  51. Li Yi, Jones Louise, McQueen-Mason Simon, Expansins and cell growth, 10.1016/j.pbi.2003.09.003
  52. Li Mingjun, Feng Fengjuan, Cheng Lailiang, Expression Patterns of Genes Involved in Sugar Metabolism and Accumulation during Apple Fruit Development, 10.1371/journal.pone.0033055
  53. Longhi Sara, Moretto Marco, Viola Roberto, Velasco Riccardo, Costa Fabrizio, Comprehensive QTL mapping survey dissects the complex fruit texture physiology in apple (Malus x domestica Borkh.)., 10.1093/jxb/err326
  54. Losada Juan M., Herrero María, Arabinogalactan-protein secretion is associated with the acquisition of stigmatic receptivity in the apple flower, 10.1093/aob/mcs116
  55. Malladi Anish, Johnson Lisa Klima, Expression profiling of cell cycle genes reveals key facilitators of cell production during carpel development, fruit set, and fruit growth in apple (Malus×domestica Borkh.), 10.1093/jxb/erq258
  56. Mann HS, Alton JJ, Kim SH, Tong CBS (2008) Differential expression of cell-wall—modifying genes and novel cDNAs in apple fruit during storage. J Am Soc Hortic Sci 133:1–6
  57. Mattioli Roberto, Marchese Daniele, D’Angeli Simone, Altamura Maria Maddalena, Costantino Paolo, Trovato Maurizio, Modulation of intracellular proline levels affects flowering time and inflorescence architecture in Arabidopsis, 10.1007/s11103-007-9269-1
  58. Meyers Blake C, Kaushik Shail, Nandety Raja Sekhar, Evolving disease resistance genes, 10.1016/j.pbi.2005.01.002
  59. Muller PY, Janovjak H, Miserez AR, Dobbie Z (2002) Processing of gene expression data generated by quantitative real-time RT-PCR. Biotechniques 32:1372–1374 1376, 1378–1379
  60. Nishizawa-Yokoi Ayako, Yabuta Yukinori, Shigeoka Shigeru, The contribution of carbohydrates including raffinose family oligosaccharides and sugar alcohols to protection of plant cells from oxidative damage, 10.4161/psb.6738
  61. Nunes Carla, Usall Josep, Teixidó Neus, Eribe Xavier Ochoa de, Viñas Immaculada, Control of post-harvest decay of apples by pre-harvest and post-harvest application of ammonium molybdate : Control of post-harvest decay of apples using ammonium molybdate, 10.1002/ps.402
  62. Park S., Identification of Genes with Potential Roles in Apple Fruit Development and Biochemistry through Large-Scale Statistical Analysis of Expressed Sequence Tags, 10.1104/pp.106.080994
  63. Pastene Edgar, Speisky Hernán, Troncoso Miriam, Alarcón Julio, Figueroa Guillermo, In Vitro Inhibitory Effect of Apple Peel Extract on the Growth ofHelicobacter pyloriand Respiratory Burst Induced on Human Neutrophils, 10.1021/jf9006592
  64. Percy Ann E., O'Brien Iona E. W., Jameson Paula E., Melton Laurence D., MacRae ELspeth A., Redgwell Robert J., Xyloglucan endotransglycosylase activity during fruit development and ripening of apple and kiwifruit, 10.1111/j.1399-3054.1996.tb00181.x
  65. Reagan-Shaw Shannon, Eggert David, Mukhtar Hasan, Ahmad Nihal, Antiproliferative Effects of Apple Peel Extract Against Cancer Cells, 10.1080/01635580903441253
  66. Redondo‐Nevado J., Moyano E., Medina‐Escobar N., Caballero J.L., Muñoz‐Blanco J., A fruit‐specific and developmentally regulated endopolygalacturonase gene from strawberry (Fragaria x ananassa cv. Chandler), 10.1093/jexbot/52.362.1941
  67. Ringli C., Monitoring the Outside: Cell Wall-Sensing Mechanisms, 10.1104/pp.110.154518
  68. Rose J. K. C., Lee H. H., Bennett A. B., Expression of a divergent expansin gene is fruit-specific and ripening-regulated, 10.1073/pnas.94.11.5955
  69. Rose Jocelyn K.C., Hadfield Kristen A., Labavitch John M., Bennett Alan B., Temporal Sequence of Cell Wall Disassembly in Rapidly Ripening Melon Fruit, 10.1104/pp.117.2.345
  70. Salentijn Elma M. J., Aharoni Asaph, Schaart Jan G., Boone Marjan J., Krens Frans A., Differential gene expression analysis of strawberry cultivars that differ in fruit-firmness, 10.1034/j.1399-3054.2003.00138.x
  71. Schimleck LR, Evans R (2004) Estimation of P. radiata D. Don tracheid morphological characteristics by near infrared spectroscopy. Holzforschung 58:66–73
  72. Schimleck Laurence R, Kube Peter D, Raymond Carolyn A, Genetic improvement of kraft pulp yield inEucalyptus nitensusing cellulose content determined by near infrared spectroscopy, 10.1139/x04-119
  73. Sherson S. M., Alford H. L., Forbes S. M., Wallace G., Smith S. M., Roles of cell-wall invertases and monosaccharide transporters in the growth and development of Arabidopsis, 10.1093/jxb/erg055
  74. Sitrit Yaron, Bennett Alan B., Regulation of Tomato Fruit Polygalacturonase mRNA Accumulation by Ethylene: A Re-Examination, 10.1104/pp.116.3.1145
  75. Smith-Moritz AM, Chern M, Lao J, Sze-To WH, Heazlewood JL, Ronald PC, Vega-Sánchez ME (2011) Combining multivariate analysis and monosaccharide composition modeling to identify plant cell wall variations by Fourier transform near infrared spectroscopy. Plant Methods 18:7–26
  76. Stone BA, Clarke AE (1992) Chemistry and biology of (1 → 3)-β-glucans. La Trobe University Press, Victoria
  77. Troyanskaya O., Cantor M., Sherlock G., Brown P., Hastie T., Tibshirani R., Botstein D., Altman R. B., Missing value estimation methods for DNA microarrays, 10.1093/bioinformatics/17.6.520
  78. Turner Simon R., Cell Walls: Monitoring Integrity with THE Kinase, 10.1016/j.cub.2007.05.033
  79. Vatén Anne, Dettmer Jan, Wu Shuang, Stierhof York-Dieter, Miyashima Shunsuke, Yadav Shri Ram, Roberts Christina J., Campilho Ana, Bulone Vincent, Lichtenberger Raffael, Lehesranta Satu, Mähönen Ari Pekka, Kim Jae-Yean, Jokitalo Eija, Sauer Norbert, Scheres Ben, Nakajima Keiji, Carlsbecker Annelie, Gallagher Kimberly L., Helariutta Ykä, Callose Biosynthesis Regulates Symplastic Trafficking during Root Development, 10.1016/j.devcel.2011.10.006
  80. Velasco Riccardo, Zharkikh Andrey, Affourtit Jason, Dhingra Amit, Cestaro Alessandro, Kalyanaraman Ananth, Fontana Paolo, Bhatnagar Satish K, Troggio Michela, Pruss Dmitry, Salvi Silvio, Pindo Massimo, Baldi Paolo, Castelletti Sara, Cavaiuolo Marina, Coppola Giuseppina, Costa Fabrizio, Cova Valentina, Dal Ri Antonio, Goremykin Vadim, Komjanc Matteo, Longhi Sara, Magnago Pierluigi, Malacarne Giulia, Malnoy Mickael, Micheletti Diego, Moretto Marco, Perazzolli Michele, Si-Ammour Azeddine, Vezzulli Silvia, Zini Elena, Eldredge Glenn, Fitzgerald Lisa M, Gutin Natalia, Lanchbury Jerry, Macalma Teresita, Mitchell Jeff T, Reid Julia, Wardell Bryan, Kodira Chinnappa, Chen Zhoutao, Desany Brian, Niazi Faheem, Palmer Melinda, Koepke Tyson, Jiwan Derick, Schaeffer Scott, Krishnan Vandhana, Wu Changjun, Chu Vu T, King Stephen T, Vick Jessica, Tao Quanzhou, Mraz Amy, Stormo Aimee, Stormo Keith, Bogden Robert, Ederle Davide, Stella Alessandra, Vecchietti Alberto, Kater Martin M, Masiero Simona, Lasserre Pauline, Lespinasse Yves, Allan Andrew C, Bus Vincent, Chagné David, Crowhurst Ross N, Gleave Andrew P, Lavezzo Enrico, Fawcett Jeffrey A, Proost Sebastian, Rouzé Pierre, Sterck Lieven, Toppo Stefano, Lazzari Barbara, Hellens Roger P, Durel Charles-Eric, Gutin Alexander, Bumgarner Roger E, Gardiner Susan E, Skolnick Mark, Egholm Michael, Van de Peer Yves, Salamini Francesco, Viola Roberto, The genome of the domesticated apple (Malus × domestica Borkh.), 10.1038/ng.654
  81. Vicente Ariel R., Ortugno Claudia, Powell Ann L. T., Greve L. Carl, Labavitch John M., Temporal Sequence of Cell Wall Disassembly Events in Developing Fruits. 1. Analysis of Raspberry (Rubus idaeus), 10.1021/jf063547r
  82. Vicente Ariel R., Ortugno Claudia, Rosli Hernán, Powell Ann L. T., Greve L. Carl, Labavitch John M., Temporal Sequence of Cell Wall Disassembly Events in Developing Fruits. 2. Analysis of Blueberry (VacciniumSpecies), 10.1021/jf063548j
  83. Wakasa Y, Hatsuyama Y, Takahashi A, Sato T, Niizeki M, Harada T (2003) Divergent expression of six expansin genes during apple fruit ontogeny. Eur J Hortic Sci 68:S253–S259
  84. Waldron K.W., Parker M.L., Smith A.C., Plant Cell Walls and Food Quality, 10.1111/j.1541-4337.2003.tb00019.x
  85. Wang Guifeng, Gao Yan, Wang Jinjun, Yang Liwei, Song Rentao, Li Xiaorong, Shi Jisen, Overexpression of two cambium-abundant Chinese fir (Cunninghamia lanceolata) α-expansin genes ClEXPA1 and ClEXPA2 affect growth and development in transgenic tobacco and increase the amount of cellulose in stem cell walls : Effects of cambium-abundant expansins on plant growth and development, 10.1111/j.1467-7652.2010.00569.x
  86. Workman J Jr, Weyer L (2007) Practical guide to near-infrared spectroscopy. CRC Press, Boca Raton
  87. Xie Bo, Wang Xiaomin, Zhu Maosheng, Zhang Zhongming, Hong Zonglie, CalS7 encodes a callose synthase responsible for callose deposition in the phloem : A phloem-specific callose synthase , 10.1111/j.1365-313x.2010.04399.x
  88. Yang Sihai, Zhang Xiaohui, Yue Jia-Xing, Tian Dacheng, Chen Jian-Qun, Recent duplications dominate NBS-encoding gene expansion in two woody species, 10.1007/s00438-008-0355-0
  89. Zenoni S., Downregulation of the Petunia hybrida  -Expansin Gene PhEXP1 Reduces the Amount of Crystalline Cellulose in Cell Walls and Leads to Phenotypic Changes in Petal Limbs, 10.1105/tpc.018705
Bibliographic reference Guerriero, Gea ; Giorno, Filimona ; Folgado, Raquel ; Printz, Bruno ; Baric, Sanja ; et. al. Callose and cellulose synthase gene expression analysis from the tight cluster to the full bloom stage and during early fruit development in Malus × domestica. In: Journal of Plant Research, Vol. 127, no.1, p. 173-183 (2014)
Permanent URL http://hdl.handle.net/2078.1/140615