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Photoactivable nonsymmetrical bifunctional linkers for protein immobilization on attenuated total reflectance FTIR optical devices

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Hammaecher, Catherine [UCL] Joris, Bernard [ULg] Goormaghtigh, Erik [ULB] Marchand-Brynaert, Jacqueline [UCL]

The photosensitive 4-azido-2,3,5,6-tetrafluoro-1-benzoyl core has been connected to maleimide-, chloroacetyloxy-, or succinimidyl carbonate motifs through various spacers to furnish a series of bifunctional linkers. One linker (7a) has been used for the construction of a biosensor for β-lactam antibiotic detection by attenuated total reflectance FTIR (ATR-FTIR) spectroscopy. Linkers featuring a photosensitive aryl azide motif at one end and a function reactive toward nucleophiles at the other end are described. One linker is used for the construction of a biosensor for β-lactam antibiotic detection by attenuated total reflectance FTIR spectroscopy. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2013
Language Anglais
Journal information "European Journal of Organic Chemistry" - Vol. 2013, no.35, p. 7952-7959 (2013)
Peer reviewed yes
Publisher Wiley - V C H Verlag GmbH & Co. KGaA ((Germany) Weinheim)
issn 1434-193X
e-issn 1099-0690
Publication status Publié
Affiliations UCL - SST/IMCN/MOST - Molecules, Solids and Reactivity
ULB - Center for Structural Biology and Bioinformatics
ULg - Centre d'Ingénierie des Protéines (CIP)
Keywords Antibiotics ; Azides ; Biosensors ; Immobilization ; IR spectroscopy ; Photoaffinity labelling
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Bibliographic reference Hammaecher, Catherine ; Joris, Bernard ; Goormaghtigh, Erik ; Marchand-Brynaert, Jacqueline. Photoactivable nonsymmetrical bifunctional linkers for protein immobilization on attenuated total reflectance FTIR optical devices. In: European Journal of Organic Chemistry, Vol. 2013, no.35, p. 7952-7959 (2013)
Permanent URL http://hdl.handle.net/2078.1/140596