User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

Transmission of the Pepino mosaic virus by whitefly

Primary tabs

Noel, Pierre [UCL] Hance, Thierry [UCL] Bragard, Claude [UCL]

Two experiments were conducted to investigate the transmission of the Pepino mosaic virus (PepMV) by the greenhouse whitefly (Trialeurodes vaporariorum) from tomato to tomato. In the 1:1 system (in which a single virus-contaminated plant was placed next to a healthy plant in a cage containing 469 whiteflies on average) the virus was transmitted to three out of 10 plants. In the 1:4 system (in which a virus-contaminated plant was surrounded by four healthy plants in a cage with 601 whiteflies on average) the virus was transmitted to five out of 32 plants. In order to investigate the mechanism involved in the transmission, the insect bodies were washed to determine the external presence of viral particles. The results showed that the number of PepMV particles carried on whitefly bodies was low, with an average occurrence of 1.33 on the 55 whiteflies tested after the insects were in contact with infected plants for 5 days. This low occurrence was confirmed by observation under microscope, which showed an absence of PepMV-contaminated tomato sap on the insect bodies, suggesting that PepMV transmission by whiteflies could occur when they feed on the plant.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2014
Language Anglais
Journal information "European Journal of Plant Pathology" - Vol. 138, no.1, p. 23-27 (2014)
Peer reviewed yes
Publisher Springer Netherlands ((Netherlands) Dordrecht)
issn 0929-1873
e-issn 1573-8469
Publication status Publié
Affiliations UCL - SST/ELI/ELIB - Biodiversity
UCL - SST/ELI/ELIM - Applied Microbiology
Keywords Pepino mosaic virus ; Trialeurodes ; Tomato ; Transmission ; Vector ; 3123 ; 3134
Links
  1. Aguilar J. M., Hernández-Gallardo M. D., Cenis J. L., Lacasa A., Aranda M. A., Complete sequence of the Pepino mosaic virus RNA genome, 10.1007/s00705-002-0848-9
  2. Alfaro-Fernández Ana, del Carmen Córdoba-Sellés María, Herrera-Vásquez José Ángel, Cebrián María del Carmen, Jordá Concepción, Transmission ofPepino mosaic virusby the Fungal VectorOlpidium virulentus, 10.1111/j.1439-0434.2009.01605.x
  3. Bragard, C., Caciagli, P., Lemaire, O., Lopez-Moya, J. J., MacFarlane, S., Peters, D., et al. (2013). Status and prospects of plant virus control through interference with Vector transmission. Annual Review of Phytopathology, 51(9), 1–25.
  4. Fakhro A., Paschek, U., Bargen, S.V., Buttner, C., & Schwarz, D. (2005). Distribution and spread of Pepino mosaic virus (PepMV) in tomatoes cultivated in a re-circulating hydroponic system. Plant protection and plant health in Europe: introduction and spread of invasive species, held at Humboldt University, Berlin, Germany, 9–11. Alton UK: British Crop Protection Council, 223–4.
  5. French C. J., Bouthillier M., Bernardy M., Ferguson G., Sabourin M., Johnson R. C., Masters C., Godkin S., Mumford R., First Report of Pepino mosaic virus in Canada and the United States, 10.1094/pdis.2001.85.10.1121b
  6. Hanssen Inge M., Paeleman Anneleen, Wittemans Lieve, Goen Kris, Lievens Bart, Bragard Claude, Vanachter Alfons C. R. C., Thomma Bart P. H. J., Genetic characterization of Pepino mosaic virus isolates from Belgian greenhouse tomatoes reveals genetic recombination, 10.1007/s10658-007-9255-0
  7. Hanssen I. M., Paeleman A., Vandewoestijne E., Van Bergen L., Bragard C., Lievens B., Vanachter A. C. R. C., Thomma B. P. H. J., Pepino mosaic virusisolates and differential symptomatology in tomato, 10.1111/j.1365-3059.2008.02018.x
  8. Hanssen Inge M., Mumford Rick, Blystad Dag-Ragnar, Cortez Isabel, Hasiów-Jaroszewska Beata, Hristova Dimitrinka, Pagán Israel, Pereira Ana-Maria, Peters Jeff, Pospieszny Henryk, Ravnikar Maja, Stijger Ineke, Tomassoli Laura, Varveri Christina, van der Vlugt René, Nielsen Steen Lykke, Seed transmission of Pepino mosaic virus in tomato, 10.1007/s10658-009-9528-x
  9. Harris Kerry F., Bath James E., The fate of pea enation mosaic virus in its pea aphid vector, Acyrthosiphon pisum (Harris), 10.1016/0042-6822(72)90432-1
  10. Hoggan, I. A. (1931). Further studies on aphid transmission of plant viruses. Phytopathology, 21, 199–212.
  11. Hoggan, I. A. (1934). Transmissibility by aphids of the Tobacco mosaic virus from different hosts. Journal Agricultural Research, 49, 1135–1142.
  12. Jones David R., 10.1023/a:1022846630513
  13. JONES R. A. C., KOENIG RENATE, LESEMANN D. E., Pepino mosaic virus, a new potexvirus from pepino (Solanum muricatum), 10.1111/j.1744-7348.1980.tb03896.x
  14. Klapwijk, J., & Stijger, C. C. M. M. (2000). Overdracht pepinomozaïekvirus door wittevlieg en Macrolophus. Groente en Fruit/Glasgroenten, 17.
  15. Ling, K.S. & Carpenter, L. (2005) Pepino mosaic virus, an emerging disease in greenhouse tomato production worldwide: is seed responsible? Acta Horticulturae. Leuven Belgium: International Society for Horticultural Science (ISHS), 43–50.
  16. Orlob, G. B. (1963). Reappraisal of Transmission of Tobacco mosaic virus by insects. Phytopathology, 53, 822–830.
  17. Pirone Thomas P., Does tobacco mosaic virus infect plants through wounds produced by aphid stylets, 10.1016/0042-6822(72)90536-3
  18. Roggero P., Masenga V., Lenzi R., Coghe F., Ena S., Winter S., First report of Pepino mosaic virus in tomato in Italy. NEW DISEASE REPORT, 10.1046/j.1365-3059.2001.00621.x
  19. Salomone, A., & Roggero, P. (2002). Host range, seed transmission and detection by ELISA and lateral flow of an Italian isolate of Pepino mosaic virus. Journal of Plant Pathology, 84(1), 65–68.
  20. Shipp J.L., Buitenhuis R., Stobbs L., Wang K., Kim W.S., Ferguson G., Vectoring of Pepino mosaic virus by bumble-bees in tomato greenhouses : Bumble-bee vectoring of PepMV, 10.1111/j.1744-7348.2008.00245.x
  21. SOLER S., PROHENS J., DIEZ M. J., NUEZ F., Natural Occurrence of Pepino mosaic virus in Lycopersicon Species in Central and Southern Peru, 10.1046/j.1439-0434.2002.00712.x
  22. van der Vlugt R. A. A., Stijger C. C. M. M., Verhoeven J. Th. J., Lesemann D.-E., First Report of Pepino Mosaic Virus on Tomato, 10.1094/pdis.2000.84.1.103c
Bibliographic reference Noel, Pierre ; Hance, Thierry ; Bragard, Claude. Transmission of the Pepino mosaic virus by whitefly. In: European Journal of Plant Pathology, Vol. 138, no.1, p. 23-27 (2014)
Permanent URL http://hdl.handle.net/2078.1/139241