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Changes in serum and intestinal diamine oxidase (DAO) activity after proximal enterectomy in rats. Correlation of DAO activity with mucosal mass parameters.

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Buts, Jean-Paul [UCL] Theys, Serge [UCL] Dekeyser, Nadine [UCL] Dive, Charles [UCL]

To determine whether serum and mucosal DAO activity reflects quantitative changes in the small bowel mucosal mass, we have chosen an experimental model of mucosal hyperplasia which is known to occur in the rat after enterectomy. A 50% proximal enterectomy or a single transection was performed in 20 growing rats weighing 145-160 g. Ten days following surgery, we determined mucosal mass parameters (weight, protein, and DNA content), sucrase activity, and DAO activity in the duodenum (segment A), proximal ileum (segment B), and distal ileum (segment C) of the remaining small intestine. Mucosal hyperplasia was demonstrated by the finding that in each segment, mucosal weight, protein, and DNA content per centimeter of gut length were significantly (P less than 0.01) higher (+38 to + 78%) in the resected group than in transected controls. In segments B and C of resected rats, the changes in DAO activity expressed per gram of mucosa paralleled the changes in mucosal mass, the activity being increased by +69% and +49% (P less than 0.05) compared to the values recorded in transected controls. Expressed per centimeter of gut length, total DAO activity was also enhanced by +141% in segment B (P less than 0.05 vs controls) and by +87% in segment C (P less than 0.01 vs controls) of resected rats. In the duodenum, the changes in DAO activity were small (+36%) and not significant.(ABSTRACT TRUNCATED AT 250 WORDS)

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Publication date 1989
Journal information "Digestive diseases and sciences" - Vol. 34, no. 9, p. 1393-8 (1989)
Peer reviewed yes
issn 0163-2116
Publication status Publié
Affiliation UCL
MESH Subject Amine Oxidase (Copper-Containing) - blood - metabolism ; Animals ; Female ; Hyperplasia - enzymology ; Intestinal Mucosa - enzymology - pathology ; Intestine, Small - enzymology - pathology - surgery ; Organ Size ; Rats ; Rats, Inbred Strains
Links
  1. Buffoni F: Histaminase and related amine oxidases. Pharmacol Rev 18:1163?1199, 1966
  2. Bieganski T: Biochemical, physiological and pathophysiological aspects of intestinal diamine oxidase. Acta Physiol Pol 34:139?154, 1983
  3. Shakir KMM, Margolis S, Baylin SB: Localisation of histaminase (diamine oxidase) in rat small intestinal mucosa: Site of release by heparin. Biochem Pharmacol 26:2343?2347, 1977
  4. D'Agostino L, D'Argenio G, Ciacci C, Daniele B, Macchia V, Mazzacca G: Diamine oxidase in rat small bowel: Distribution in different segments and cellular location. Enzyme 31:217?220, 1984
  5. Baylin SB, Stevens SA, Shakir KMM: Association of diamine oxidase and ornithine decarboxylase with maturating cells in rapidly proliferating epithelium. Biochim Biophys Acta 541:415?419, 1978
  6. Lin CV, Kirley SD: Human placental and tumor histaminases.In Proteins of Biological Fluids. J Peeters (ed). Oxford, Pergamon Press, 1976, p 103
  7. Jänne J, Pösö H, Raina A: Polyamines in rapid growth and cancer. Biochim Biophys Acta 473:241?293, 1978
  8. Luk GD, Bayless TM, Baylin SB: Diamine oxidase (DAO) a circulating marker for rat mucosal maturation and integrity. J Clin Invest 66:66?70, 1980
  9. Forget P, Grandfils, Vant Cutsem JL, Dandrifosse G: Diamine oxidase and disaccharidase activities in small intestinal biopsies of children. Pediatr Res 18:647?649, 1984
  10. Forget P, Saye Z, Van Cutsem JL, Dandrifosse G: Serum diamine oxidase activity in acute gastroenteritis in children. Pediatr Res 19:26?28, 1984
  11. Forget P, Grandfils C, Van Cutsem JL, Dandrifosse G: Diamine oxidase in serum and small intestinal biopsy tissue in childhood celiac disease. J Pediatr Gastroenterol Nutr 5:379?383, 1986
  12. D'Agostino L, Ciacci C, Daniele B, Barone MV, Sollazo R, Mazzacca G: Postheparin plasma diamine oxidase in subjects with small bowel mucosal atrophy. Dig Dis Sci 32:313?317, 1987
  13. Bayless TM, Luk GD, Baylin SB, Thomas ME: Decreased plasma diamine oxidase levels in patients with Crohn's disease and celiac disease. Gastroenterology 80:1106, 1981
  14. Buts JP, Morin CL, Ling V: Influence of dietary components on intestinal adaptation after small bowel resection in rats. Clin Invest Med 2:59?66, 1979
  15. Okuyama T, Kobayashi Y: Determination of diamine oxidase activity by liquid scintillation counting. Arch Biochem Biophys 95:242?250, 1961
  16. Messer M, Dahlqvist A: A one-step ultramicromethod for the assay of intestinal disaccharidases. Anal Biochem 14:376?392, 1966
  17. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 193:265?275, 1951
  18. Burton K: A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of desoxyribonucleic acid. Biochem J 62:315?323, 1956
  19. Giles WK, Myers M: An improved diphenylamine method for the estimation of DNA. Nature 206:93?94, 1965
  20. Buts JP, De Keyser N, Dive CH: Cellular adaptation of the rat small intestine after proximal enterectomy: changes in microvillous enzymes and in the secretory component of immunoglobulins. Pediatr Res 22:29?33, 1987
  21. Williamson RCN: Intestinal adaptation: Structural, functional and cytokinetic changes. N Engl J Med 298:1393?1399, 1978
  22. Nygaard K: Resection of the small intestine in rat III morphological changes in the intestinal tract. Acta Chir Scand 133:233?248, 1967
  23. Dowling RH, Booth CC: Structural and functional changes following small intestinal resection in the rat. Clin Sci 32:139?149, 1967
  24. Hanson WR, Osborne JW, Sharp JG: Compensation by the residual intestine after intestine resection in the rat II. Influence of postoperative time interval. Gastroenterology 72:701?705, 1977
  25. Weser E, Tawil T: Epithelial cell loss in remaining intestine after small bowel resection in the rat. Gastroenterology 71:412?415, 1976
  26. Menge H, Robinson JWL: The relationship between the functional and structural alterations in the rat small intestine following proximal resection of varying extents. Res Exp Med (Berlin) 173:41?53, 1978
  27. Mc Carthy DM, Kim SY: Changes in sucrase, enterokinase and peptide hydrolase after intestinal resection. J Clin Invest 52:942?951, 1973
Bibliographic reference Buts, Jean-Paul ; Theys, Serge ; Dekeyser, Nadine ; Dive, Charles. Changes in serum and intestinal diamine oxidase (DAO) activity after proximal enterectomy in rats. Correlation of DAO activity with mucosal mass parameters.. In: Digestive diseases and sciences, Vol. 34, no. 9, p. 1393-8 (1989)
Permanent URL http://hdl.handle.net/2078.1/13882