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Spatiotemporal control of PopZ localization through cell cycle-coupled multimerization

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Laloux, Géraldine [UCL] Jacobs-Wagner, Christine [Yale University]

Bacterial cell poles constitute defined subcellular domains where numerous proteins localize, often at specific times, to affect various physiological processes. How pole recognition occurs and what governs the timing of protein localization are often unknown. In this paper, we investigate the mechanisms governing the localization of PopZ, a chromosome-anchoring protein whose unipolar to bipolar localization pattern is critical for cell cycle progression in Caulobacter crescentus. We provide evidence that polar localization of PopZ relied on its self-assembly into a higher-order structure (matrix) and that the unipolar to bipolar transition was coupled to the asymmetric distribution of ParA during the translocation of the origin-proximal ParB–parS partition complex. Collectively, our data suggest a model in which a local increase of ParA concentration promotes the assembly of a PopZ matrix precisely when and where this matrix is needed. Such coupling of protein assembly with a cell cycle–associated molecular asymmetry may represent a principle of cellular organization for controlling protein localization in both time and space.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 2013
Language Anglais
Journal information "The Journal of Cell Biology" - Vol. 201, no.6, p. 827-841 (10/06/2013)
Peer reviewed yes
issn 0021-9525
e-issn 1540-8140
Publication status Publié
Affiliations Yale University - Department of Molecular, Cellular and Developmental Biology
Yale University - Howard Hughes Medical Institute
Yale School of Medicine - Department of Microbial Pathogenesis
Links
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Bibliographic reference Laloux, Géraldine ; Jacobs-Wagner, Christine. Spatiotemporal control of PopZ localization through cell cycle-coupled multimerization. In: The Journal of Cell Biology, Vol. 201, no.6, p. 827-841 (10/06/2013)
Permanent URL http://hdl.handle.net/2078/138577