Everard, Amandine
[UCL]
Delzenne, Nathalie M.
[UCL]
Cani, Patrice D.
[UCL]
Obesity and related metabolic disorders such as insulin resistance and type 2 diabetes are associated with a low-grade inflammatory state. These disorders may be related to the gut microbiota possibly through the development of metabolic endotoxemia (i.e., increased plasma lipopolysaccharide levels (LPS)). This higher plasma LPS is associated with gut permeability. We have also previously shown that gut microbiota modulations (i.e., prebiotics) improve gut barrier functions, inflammation and metabolic disorders. However, no specific bacteria have been causally linked with these beneficial effects. More recently, by using metagenomic approaches, we found that prebiotic treatment dramatically increases Akkermansia muciniphila in genetic obese mice. The aim of this study was 1° to investigate the effect of high-fat diet-induced obesity and type 2 diabetes on this bacteria, 2° to evaluate the impact of prebiotic on the abundance of Akkermansia muciniphila in this model, 3° to unravel its implication on gut barrier functions, inflammation and metabolism. In this study we show for the first time that Akkermansia muciniphila is dramatically decreased upon high-fat diet (83-fold decrease), whereas its abundance was dramatically increased after prebiotic treatment (294-fold increase). Moreover this bacteria was inversely correlated with several markers of inflammation in the gut (TLR4 and IL-1 beta mRNA), the liver (Myd88 and IL-1 beta mRNA) and the adipose tissue (CD11c mRNA). We also found that Akkermansia muciniphila correlates positively with muscle mass and negatively with adiposity and body weight gain. Importantly, we are currently investigating the impact of Akkermansia muciniphila administration in several obese and type 2 diabetes models. In conclusion, we propose Akkermansia muciniphila as a novel bacteria causally linked with gut barrier, whole body inflammation and metabolism in obesity.


Bibliographic reference |
Everard, Amandine ; Delzenne, Nathalie M. ; Cani, Patrice D.. Akkermansia muciniphila link gut barrier function with inflammation and metabolic disorders associated with obesity. .Keystone Symposia: The Microbiome (Q8) (Keystone, USA, du 04/03/2012 au 09/03/2012). |
Permanent URL |
http://hdl.handle.net/2078.1/138299 |