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B cell adrenoceptors and sulphonylurea-induced insulin release in mouse islets.

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Garrino, M G Henquin, Jean-Claude [UCL]

Interactions of tolbutamide and glibenclamide with B cell adrenoceptors have been reported. This study evaluated the possible role of such interactions in the stimulation of insulin release. Mouse islets were incubated in the presence of 10 mmol/l glucose alone or with tolbutamide (10 mumol/l) or glibenclamide (0.02 mumol/l). At 0.01-10 mumol/l, blockers of alpha 2-adrenoceptors (yohimbine, idazoxan) or alpha 1-adrenoceptors (prazosin) had practically no effect on glucose-induced insulin release and did not affect its potentiation by sulphonylureas, except for a slight increase by 10 mumol/l prazosin and idazoxan. Nonspecific alpha-blockers (phentolamine, dihydroergotamine) increased control release at 10 mumol/l, but only the latter amplified the response to tolbutamide. Blockers of beta-adrenoceptors were tested at 0.1-100 mumol/l: propranolol (beta 1, beta 2), metoprolol (beta 1) and compound ICI 118-551 (beta 2). They increased glucose-induced insulin release at 100 mumol/l but variably altered the effect of sulphonylureas. Blockers of adrenoceptors have, thus, no effect on insulin release in vitro at therapeutic concentrations. At high concentrations, they non-specifically affect the action of sulphonylureas. We conclude that an interaction with B cell adrenoceptors is not involved in the insulinotropic action of sulphonylureas.

Document type Article de périodique (Journal article) – In Vitro, Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 1990
Journal information "Diabetologia" - Vol. 33, no. 3, p. 145-7 (1990)
Peer reviewed yes
issn 0012-186X
Affiliation UCL - MD/FSIO - Département de physiologie et pharmacologie
MESH Subject Adrenergic alpha-Antagonists - pharmacology ; Adrenergic beta-Antagonists - pharmacology ; Animals ; Female ; Glyburide - pharmacology ; Insulin - secretion ; Islets of Langerhans - drug effects - physiology - secretion ; Kinetics ; Metoprolol - pharmacology ; Mice ; Mice, Inbred Strains ; Propanolamines - pharmacology ; Propranolol - pharmacology ; Receptors, Adrenergic, beta - drug effects - physiology ; Tolbutamide - pharmacology
Links
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Bibliographic reference Garrino, M G ; Henquin, Jean-Claude. B cell adrenoceptors and sulphonylurea-induced insulin release in mouse islets.. In: Diabetologia, Vol. 33, no. 3, p. 145-7 (1990)
Permanent URL http://hdl.handle.net/2078.1/13825