User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

Interferon-gamma and interleukin-1 beta inhibit adipoconversion in cultured rodent preadipocytes.

Primary tabs

Grégoire, F De Broux, N Hauser, N. Heremans, H. Van Damme, Julie [UCL] Remacle, Claude [UCL]

Cytokines like tumor necrosis factor (TNF), interferon-gamma (IFN-gamma), and interleukin-1 (IL-1) are known to interfere with the differentiation of cultured cell lines of adipocyte precursors. In the present study, the effect of mouse and rat IFN-gamma, as well as human IL-1 beta, was investigated on rodent preadipocytes in primary cultures, either in the presence of fetal bovine serum (FBS, 10%) or in serum-free defined medium. IFN-gamma exerted an antiproliferative action that was more pronounced when cells reached confluency than during the growth phase of the culture. Morphological observation and quantifications of undifferentiated and differentiating cells revealed that IFN-gamma caused a decrease in the proportion of cells devoid of lipid droplets which would correspond to fibroblast-like cells, whereas preadipocytes remained unaffected. IFN-gamma induced a marked retardation of adipoconversion, resulting in a partial inhibition of lipoprotein lipase (LPL) activity and a severe decrease in glycerol-3-phosphate dehydrogenase (GPDH) activity. The antiproliferative and anti-LPL effects of IFN-gamma were neutralized by adding anti-IFN-gamma antibodies, while these antibodies prevented only partially the depressing effect of IFN-gamma on GPDH activity. Contrary to IFN-gamma, IL-1 beta slightly enhanced the proliferation in preadipocyte cultures. IL-1 beta also depressed adipoconversion, inhibited markedly LPL activity, and partially reduced GPDH activity. These results show that the influence of cytokines on adipoconversion observed in preadipocyte cell lines can be found in normal preadipocytes in culture.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Publication date 1992
Journal information "Journal of cellular physiology" - Vol. 151, no. 2, p. 300-9 (1992)
Peer reviewed yes
issn 0021-9541
Publication status Publié
Affiliation UCL - SC/BIOL - Département de biologie
MESH Subject Adipose Tissue - cytology ; Animals ; Cell Differentiation - drug effects ; Cell Division - drug effects ; Cells, Cultured ; Interferon Type II - pharmacology ; Interleukin-1 - pharmacology ; Rodentia ; Stem Cells - cytology
Links
  1. Bazin Hervé, Xhurdebise Louis-Marie, Burtonboy Guy, Lebacq Anne-Marie, De Clercq Lieve, Cormont Françoise, Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants, 10.1016/0022-1759(84)90337-5
  2. Beutler, N. Engl. J. Med., 316, 379 (1987)
  3. Björntorp, J. Lipid Res., 19, 316 (1978)
  4. Björntorp, J. Lipid Res., 1, 714 (1980)
  5. Bradford, Anal. Biochem., 72, 248 (1976)
  6. CIOE L, OBRIEN T, DIAMOND L, Inhibition of adipose conversion of BALB/c 3T3 cells by interferon and 12-O-tetradecanoylphorbol-13-acetate, 10.1016/0309-1651(80)90057-0
  7. Cornelius, Biochem. J., 249, 765 (1988)
  8. Cure, Methods in Cell Biology, 14, 125 (1976)
  9. Deslex Sylviane, Negrel Raymond, Ailhaud Gérard, Development of a chemically defined serum-free medium for differentiation of rat adipose precursor cells, 10.1016/0014-4827(87)90412-5
  10. Dijkmans, J. Interferon Res., 5, 511 (1987)
  11. Dijkmans, Infect. Immun., 58, 32 (1990)
  12. Dobson, J. Biol. Chem., 262, 1804 (1987)
  13. Elias, Am. Rev. Respir. Dis., 138, 652 (1988)
  14. Ferreira, Science, 232, 881 (1986)
  15. Gaben-Cogneville Anne-Marie, Aron Yolande, Idriss Ginette, Jahchan Thérèse, Pello Jean-Yves, Swierczewski Elisabeth, Differentiation under the control of insulin of rat preadipocytes in primary culture, 10.1016/0167-4889(83)90009-5
  16. Gre´goire Francine, Todoroff Germaine, Hauser Nadine, Remacle Claude, The stroma-vascular fraction of rat inguinal and epididymal adipose tissue and the adipoconversion of fat cell precursors in primary culture, 10.1016/0248-4900(90)90348-7
  17. Grégoire Francine, Genart Christine, Hauser Nadine, Remacle Claude, Glucocorticoids induce a drastic inhibition of proliferation and stimulate differentiation of adult rat fat cell precursors, 10.1016/0014-4827(91)90261-r
  18. Hauner, J. Clin. Invest., 84, 1663 (1989)
  19. Heremans, J. Immunol., 138, 4175 (1987)
  20. Heremans, Dev. Biol. Stand., 71, 113 (1989)
  21. Ijzermans, Immunobiology, 179, 456 (1989)
  22. KAWAKAMI Masanobu, MURASE Toshio, OGAMA Hiroko, ISHIBASHI Shun, MORI Natsuko, TAKAKU Fumimaro, SHIBATA Seiichi, Human Recombinant TNF Suppresses Lipoprotein Lipase Activity and Stimulates Lipolysis in 3T3-L1 Cells1, 10.1093/oxfordjournals.jbchem.a121917
  23. Kawakami, J. Cell. Physiol., 138, 1 (1989)
  24. Kawakami, Ann. N. Y. Acad. Sci., 587, 339 (1990)
  25. Keay, Proc. Natl. Acad. Sci. U.S.A., 77, 4099 (1980)
  26. Ogawa, Biochim. Biophys. Acta, 1003, 131 (1989)
  27. Opdenakker, Experientia, 45, 513 (1989)
  28. Pape, Mol. Cell. Biol., 9, 974 (1989)
  29. Patton, Proc. Natl. Acad. Sci. U.S.A., 83, 8313 (1986)
  30. Price, Biochem. J., 240, 601 (1986)
  31. Roncari, Biochem. Cell. Biol., 68, 238 (1990)
  32. Taylor, J. Biol. Chem., 263, 1336 (1988)
  33. Van Damme J., De Ley M., Opdenakker G., Billiau A., De Somer P., Beeumen J. Van, Homogeneous interferon-inducing 22K factor is related to endogenous pyrogen and interleukin-1, 10.1038/314266a0
  34. Van Damme J., Identification of the human 26-kD protein, interferon beta 2 (IFN-beta 2), as a B cell hybridoma/plasmacytoma growth factor induced by interleukin 1 and tumor necrosis factor, 10.1084/jem.165.3.914
  35. Van Damme, J. Immunol., 140, 1534 (1988)
  36. Vannier, J. Biol. Chem., 260, 4424 (1985)
  37. Wise, J. Biol. Chem., 254, 273 (1979)
  38. Zechner, Mol. Cell. Biol., 8, 2394 (1988)
Bibliographic reference Grégoire, F ; De Broux, N ; Hauser, N. ; Heremans, H. ; Van Damme, Julie ; et. al. Interferon-gamma and interleukin-1 beta inhibit adipoconversion in cultured rodent preadipocytes.. In: Journal of cellular physiology, Vol. 151, no. 2, p. 300-9 (1992)
Permanent URL http://hdl.handle.net/2078.1/13649