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Impairment of growth of Leishmania donovani by Trypanosoma brucei during co-culture.

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Coppens, Isabelle [UCL] Ter Kuile, B. H. [UCL] Opperdoes, Frederik [UCL]

Cells of Leishmania donovani in co-culture with Trypanosoma brucei, were severely affected in their growth, resulting in swelling and subsequent lysis. Similar effects were also observed when Crithidia luciliae or Phytomonas sp. were co-cultured with T. brucei. Direct contact between the cells under investigation and T. brucei was necessary because T. brucei did not hamper the growth of the other trypanosomatids, when separated by a filter with 0.2 microns pore size. Examination of this phenomenon at the ultrastructural level, in a co-culture of L. donovani and T. brucei, suggests that the plasma membrane permeability is increased in the former, as a result of a close cellular contact between the two cell types.

Document type Article de périodique (Journal article) – Journal Article
Publication date 1992
Journal information "Parasitology" - Vol. 105 ( Pt 3), p. 393-8 (1992)
Peer reviewed yes
issn 0031-1820
Publication status Publié
Affiliation UCL - MD/BICL - Département de biochimie et de biologie cellulaire
MESH Subject Animals ; Cell Adhesion ; Crithidia - growth & development ; Culture Media ; Leishmania donovani - growth & development - ultrastructure ; Microscopy, Electron ; Trypanosoma brucei brucei - growth & development - ultrastructure ; Trypanosomatina - growth & development
Links
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Bibliographic reference Coppens, Isabelle ; Ter Kuile, B. H. ; Opperdoes, Frederik. Impairment of growth of Leishmania donovani by Trypanosoma brucei during co-culture.. In: Parasitology, Vol. 105 ( Pt 3), p. 393-8 (1992)
Permanent URL http://hdl.handle.net/2078.1/13612