Wolucka, Béata
[UCL]
McNeil, Michael R.
[Department of Microbiology, Colorado State University, Fort Collins, CO, USA]
Kalbe, Luise-Dorothea
[UCL]
Cocito, Carlo
[UCL]
Brennan, Patrick J.
[Department of Microbiology, Colorado State University, Fort Collins, CO, USA]
A glucuronic acid-containing diacylglycerol was isolated from exponentially growing Mycobacterium smegmatis. Structural analysis of the purified glycolipid, performed by gas chromatography-mass spectrometry, fast atom bombardment-mass spectrometry, and high resolution proton NMR, indicated the structure 3-(O-alpha-D-glucuronopyranosyl)-1,2-diacyl-sn-glycerol. Two forms of the glycolipid were observed differing in fatty acid composition. Both molecular species contained a hexadecanoic acid residue, whereas the second acyl group was either tuberculostearic acid (10-methylstearic acid) or octadecenoic acid. The inherent antigenicity of the glycolipid was shown by its ability to bind to anti-Mycobacterium avium (serovar 26) and anti-Mycobacterium tuberculosis sera by Western blot-type thin-layer chromatography. This is the second instance of the isolation of a glycosyl diacylglycerol from members of the Mycobacterium genus, further confirming its close relationship to Gram-positive bacteria.
Bibliographic reference |
Wolucka, Béata ; McNeil, Michael R. ; Kalbe, Luise-Dorothea ; Cocito, Carlo ; Brennan, Patrick J.. Isolation and characterization of a novel glucuronosyl diacylglycerol from Mycobacterium smegmatis.. In: Biochimica et biophysica acta, Vol. 1170, no. 2, p. 131-6 (1993) |
Permanent URL |
http://hdl.handle.net/2078.1/13524 |