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Use of MSAP Markers to Analyse the Effects of Salt Stress on DNA Methylation in Rapeseed (Brassica napus var. oleifera)

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Marconi, Gianpiero [Department of applied Biology, University of Perugia, Italy] Pace, Roberta [Department of agricultural and environmental science, University of Perugia, Italy] Traini, Alessandra [Department of soil, plant, environmental and animal production sciences, University of Naples] Raggi, Lorenzo [Department of applied Biology, University of Perugia, Italy] Lutts, Stanley [UCL] Albertini, Emidio [Department of applied Biology, University of Perugia, Italy]

Excessive soil salinity is a major ecological and agronomical problem, the adverse effects of which are becoming a serious issue in regions where saline water is used for irrigation. Plants can employ regulatory strategies, such as DNA methylation, to enable relatively rapid adaptation to new conditions. In this regard, cytosine methylation might play an integral role in the regulation of gene expression at both the transcriptional and post-transcriptional levels. Rapeseed, which is the most important oilseed crop in Europe, is classified as being tolerant of salinity, although cultivars can vary substantially in their levels of tolerance. In this study, the Methylation Sensitive Amplified Polymorphism (MSAP) approach was used to assess the extent of cytosine methylation under salinity stress in salinity-tolerant (Exagone) and salinity-sensitive (Toccata) rapeseed cultivars. Our data show that salinity affected the level of DNA methylation. In particular methylation decreased in Exagone and increased in Toccata. Nineteen DNA fragments showing polymorphisms related to differences in methylation were sequenced. In particular, two of these were highly similar to genes involved in stress responses (Lacerata and trehalose-6-phosphatase synthase S4) and were chosen to further characterization. Bisulfite sequencing and quantitative RT-PCR analysis of selected MSAP loci showed that cytosine methylation changes under salinity as well as gene expression varied. In particular, our data show that salinity stress influences the expression of the two stress-related genes. Moreover, we quantified the level of trehalose in Exagone shoots and found that it was correlated to TPS4 expression and, therefore, to DNA methylation. In conclusion, we found that salinity could induce genome-wide changes in DNA methylation status, and that these changes, when averaged across different genotypes and developmental stages, accounted for 16.8% of the total site-specific methylation differences in the rapeseed genome, as detected by MSAP analysis. © 2013 Marconi et al.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 2013
Language Anglais
Journal information "PLoS One" - Vol. 8, no.9, p. 1-16 (2013)
Peer reviewed yes
Publisher Public Library of Science ((United States) [S.l.])
e-issn 1932-6203
Publication status Publié
Affiliation UCL - SST/ELI/ELIA - Agronomy
Links
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Bibliographic reference Marconi, Gianpiero ; Pace, Roberta ; Traini, Alessandra ; Raggi, Lorenzo ; Lutts, Stanley ; et. al. Use of MSAP Markers to Analyse the Effects of Salt Stress on DNA Methylation in Rapeseed (Brassica napus var. oleifera). In: PLoS One, Vol. 8, no.9, p. 1-16 (2013)
Permanent URL http://hdl.handle.net/2078.1/134242