User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

Depletion of IgM xenoreactive natural antibodies by injection of anti-mu monoclonal antibodies

Primary tabs

Latinne, Dominique [UCL] Soares, Miguel Havaux, Xavier [UCL] Cormont, Françoise Lesnikoski, Beth Bazin, Hervé [UCL]

It is believed that IgM xenoreactive natural antibodies (XNA) and activation of complement are the two main effectors involved in the hyperacute rejection (HAR) of discordant xenografts, such as pig-to-primate kidney, liver or heart transplants. We have hypothesized that long-term depletion of circulating IgM XNA might be able to overcome HAR and induce the "accommodation" of pig-to-primate vascular discordant xenografts. Several techniques have been described to eliminate circulating XNA in primates but, up to now, none has been able to totally deplete these antibodies for a sufficiently long period of time in order to test the hypothesis of discordant xenograft "accommodation". Previous reports from our laboratory have shown that, in rodents, B-cell immunosuppression could be achieved by neonatal administration of anti-mu antibodies. Recently we have shown that administration of an anti-mu mAb, in adult rats, was able to totally deplete circulating IgM and IgM XNA, without immune complex disease. Furthermore, we have used different methods such as splenectomy, plasma exchange and an anti-B cell immunosuppressive agent mycophenylate mophetil (RS61443, Syntex, Palo Alto, USA) to pre-deplete circulating IgM before administration of anti-mu mAb (MARM-7) and showed that the effectiveness of anti-mu mAb to deplete circulating IgM was increased by 100-fold. Depletion of circulating IgM in adult rats by anti-mu mAb (MARM-7) was used as an experimental model to study the role of IgM XNA in the pathogenesis of HAR in guinea pig-to-rat cardiac xenografts. Our data show that IgM XNA play a major role in HAR, even if in this discordant combination direct activation of complement, probably through the alternative pathway, seems to be the main effector involved in HAR. We have analyzed the mechanisms of anti-mu depletion of circulating IgM in adult animals and shown that, besides anti-mu/IgM immune complex formation, depletion of circulating IgM results from the very significant inhibition of B-cell differentiation and secretion of IgM following in vivo crosslinking and internalization of surface IgM on B cells. As well, we provide evidence demonstrating that anti-mu mAb blocks B cells at an early stage of maturation, probably in the bone marrow. Furthermore, we have developed several rat anti-human and anti-baboon IgM mAb and tested their ability to deplete circulating IgM and IgM XNA in baboons, after splenectomy or splenectomy and plasma exchange.(ABSTRACT TRUNCATED AT 400 WORDS)

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 1994
Journal information "Immunological Reviews" - no. 141, p. 95-125 (1994)
Peer reviewed yes
Publisher Wiley-Blackwell Publishing, Inc. (Malden)
issn 0105-2896
e-issn 1600-065X
Publication status Publié
Affiliations UCL - MD/CHIR - Département de chirurgie
UCL - (SLuc) Service de biologie hématologique
UCL - (SLuc) Centre de thérapie tissulaire et cellulaire
UCL - MD/MINT - Département de médecine interne
MESH Subject Animals ; Antibodies, Monoclonal - administration & dosage ; B-Lymphocytes - immunology ; Graft Rejection - immunology - prevention & control ; Humans ; Immunoglobulin M - immunology ; Immunoglobulin mu-Chains - immunology ; Injections ; Lymphocyte Depletion ; Transplantation, Heterologous - immunology
Links
  1. Abney E. R., J. Immunol., 120, 2041 (1978)
  2. Alexandre G. P J., Xenograft, 25, 259 (1989)
  3. Alexandre G. P J., Transplant. Rev., 5, 230 (1991)
  4. Alexandre G. P J., Clin. Transplantation, 5, 583 (1991)
  5. Alexandre G. P J., Transplant. Proc., 19, 4538 (1987)
  6. AVRAMEAS S, Natural autoantibodies: from ?horror autotoxicus? to ?gnothi seauton?, 10.1016/0167-5699(91)90080-d
  7. Bach F. H., Transplant. Proc., 19, 4538 (1991)
  8. Bannett A. D., Transplant. Proc., 19, 4543 (1987)
  9. Bazin H., Eur. J. Immunol, 4, 44 (1974)
  10. Bazin H., J. Immunol., 121, 2077 (1978)
  11. Bazin H., Ann. N. Y. Acad. Sci., 399, 157 (1982)
  12. Bazin H., J. Immunol., 121, 2083 (1978)
  13. Bazin H., Immunology, 54, 79 (1985)
  14. Bazin H., Rat Hybridomas and Rat Monoclonal Antibodies, 1, 5 (1990)
  15. Beckers Andrée, Bazin Hervé, Allotypes of rat immunoglobulins—III: An allotype of the γ2b-chain locus, 10.1016/0019-2791(75)90214-1
  16. Borrebaeck Carl A.K., Malmborg Ann-Christin, Ohlin Mats, Does endogenous glycosylation prevent the use of mouse monoclonal antibodies as cancer therapeutics?, 10.1016/0167-5699(93)90259-n
  17. Bouwman E., Transplant. Proc., 22, 1063 (1990)
  18. Brink R., Immunoglobulin M and D antigen receptors are both capable of mediating B lymphocyte activation, deletion, or anergy after interaction with specific antigen, 10.1084/jem.176.4.991
  19. T Cairns, E. Karlsson, J. Holgersson, K. Welsh, and B. Samuelsson (1993 ) Confirmation of a major target epitope of human natural IgG and IgM anti-pig antibodies; terminal galactosea 1.3 galactose .Second international Congress in Xenotransplantation. Abstracts Sept. 26-29.pp.56 .
  20. Calne R. T., Transplant. Proc., 2, 550 (1970)
  21. Calcert J. E., J. Immunol, 1, 655 (1983)
  22. Carsetti R., Eur. J. Immunol., 23, 168 (1993)
  23. Casali Paolo, Notkins Abner L., CD5+ B lymphocytes, polyreactive antibodies and the human B-cell repertoire, 10.1016/0167-5699(89)90268-5
  24. Cattell Victoria, Jamieson S. W., Hyperacute rejection of guinea-pig to rat cardiac xenografts. I. Morphology, 10.1002/path.1711150309
  25. Chan E. Y. T, Eur. J. Immunol., 23, 357 (1993)
  26. Cooper D. K. C, Transplantation, 56, 769 (1993)
  27. Cosimi A. B., Transplantation, 32, 535 (1981)
  28. Deenen G. J., Eur. J. Immunol, 20, 557 (1990)
  29. Deenen G. J., Int. Immunol., 5, 735 (1993)
  30. Denis O., Scand. J. Immunol., 39, 625 (1994)
  31. Balian G. P, J. Exp. Med., 152, 29 (1980)
  32. Dijkstra C. D., Immunology, 54, 589 (1985)
  33. Fishel R. J., Transplant. Proc., 23, 589 (1990)
  34. Fuji H., Ann. Inst. Pasteur, 117, 801 (1969)
  35. Galili Uri, Interaction of the natural anti-Gal antibody with α-galactosyl epitopes: a major obstacle for xenotransplantation in humans, 10.1016/0167-5699(93)90261-i
  36. Galili U., A unique natural human IgG antibody with anti-alpha-galactosyl specificity, 10.1084/jem.160.5.1519
  37. Gambiez L., Transplantation, 54, 577 (1992)
  38. Gaur A., J. Immunol, 150, 1663 (1993)
  39. Good A. H., Transplant. Proc., 24, 559 (1992)
  40. Goodnow C., Transgenic Mice And Analysis Of B-Cell Tolerance, 10.1146/annurev.immunol.10.1.489
  41. Goodnow Christopher C., Crosbie Jeffrey, Adelstein Stephen, Lavoie Thomas B., Smith-Gill Sandra J., Brink Robert A., Pritchard-Briscoe Helen, Wotherspoon John S., Loblay Robert H., Raphael Kathy, Trent Ronald J., Basten Antony, Altered immunoglobulin expression and functional silencing of self-reactive B lymphocytes in transgenic mice, 10.1038/334676a0
  42. Grandien A., Eur. J. Immunol, 23, 1561 (1993)
  43. Gray D., Population kinetics of rat peripheral B cells, 10.1084/jem.167.3.805
  44. Gray D., Clin. Exp. Immunol., 60, 78 (1985)
  45. Gray D., Eur. J. Immunol, 12, 564 (1982)
  46. Gray D, Ewr. J. Immunol., 14, 47 (1984)
  47. Hammer C., Transplant. Proc., 19, 4443 (1987)
  48. Hartley Suzanne B., Cooke Michael P., Fulcher David A., Harris Alan W., Cory Suzanne, Basten Antony, Goodnow Christopher C., Elimination of self-reactive B lymphocytes proceeds in two stages: Arrested development and cell death, 10.1016/0092-8674(93)90111-3
  49. Hartmann K. U., Immunology, 20, 29 (1971)
  50. Ho F, Eur. J. Immunol., 16, 1297 (1986)
  51. Hunt S. V, Eur. J. Immunol, 7, 817 (1977)
  52. Inverardi L., J. Immunol, 149, 1416 (1992)
  53. Ishizaka K., J. Immunol, 120, 655 (1978)
  54. Kim K. M., J. Immunol., 148, 29 (1992)
  55. Kincade P. W, Proc. Natl Acad. Sci., 67, t9t8 (1970)
  56. Kincade P. W., J. Immunol., 106, 1421 (1971)
  57. Kirk A. D., Transplantation, 56, 785 (1993)
  58. Kroese F. G. M., Eur. J. Immunol., 20, 1527 (1990)
  59. Latinne D., Transplant. Proc., 25, 336 (1993)
  60. Latinne D, Transplantation, 57, 238 (1994)
  61. Lefebvre M., Rat Hybridomas and Rat Monoclonal Antibodies, 235 (1990)
  62. Lesley J., Science, 169, 487 (1970)
  63. Leslie G. A., J. Immunol, 110, 959 (1973)
  64. Leventhal J. R., Transplantation, 55, 857 (1993)
  65. Leventhal J. R., Transplant. Proc., 24, 455 (1992)
  66. Leventhal J. R., Transplantation, 54, 26 (1992)
  67. Linton R. J., Cell, 59 (1989)
  68. MacLennan I.C.M., Gray D., Kumararatne D.S., Bazin H., The lymphocytes of splenic marginal zones: a distinct B-cell lineage, 10.1016/0167-5699(82)90032-9
  69. Manning D. D., J. Immunol., 109, 1152 (1972)
  70. Manning D. D., J. Immunol, 113, 455 (1974)
  71. Manning D. D., J. Immunol, 118, 1109 (1977)
  72. Manning D. D., J. Immunol, 108, 282 (1972)
  73. Manning D. D., Suppression of reaginic antibody (IgE) formation in mice by treatment with anti-mu antiserum, 10.1084/jem.144.1.288
  74. Makowka L., Xeno, 1, 27 (1993)
  75. Miyagawa S., Transplantation, 46, 825 (1988)
  76. Mosmann Tim, Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays, 10.1016/0022-1759(83)90303-4
  77. Nemazee David, Promotion and prevention of autoimmunity by B lymphocytes, 10.1016/0952-7915(93)90098-d
  78. Nemazee David A., Bürki Kurt, Clonal deletion of B lymphocytes in a transgenic mouse bearing anti-MHC class I antibody genes, 10.1038/337562a0
  79. Opstelten D., J. Immunol., 137, 76 (1986)
  80. Oriol R., Transplant. proc., 19, 4416 (1987)
  81. Oriol R., Transplantation, 56, 1433 (1993)
  82. Osmond Dennis G, The turnover of B-cell populations, 10.1016/0167-5699(93)90322-c
  83. Osmond D.G., Nossal G.J.V., Differentiation of lymphocytes in mouse bone marrow, 10.1016/0008-8749(74)90233-0
  84. Parry S. L., Eur. J. Immunol., 24, 974 (1994)
  85. Peleman Renaat, Delespesse Guy, In vitro synthesis of human IgE by neonatal lymphocytes: Enhancing effect of interferon-γ, 10.1016/0008-8749(90)90206-7
  86. Platt J. L., Transplantation, 49, 1000 (1990)
  87. Platt J. L., Transplantation, 52, 937 (1991)
  88. Platt J. L., Eur. J. Immunol., 21, 2887 (1991)
  89. Platt J. L., Transplantation, 52, 214 (1991)
  90. Platt J. L., Transplantation, 57, 327 (1994)
  91. Platt J. L., Transplantation, 50, 817 (1990)
  92. Platt J. L., Transplantation, 52, 1037 (1991)
  93. Pruitt S. K., Transplantation, 52, 868 (1991)
  94. Pruitt S. K., Transplantation, 56, 1318 (1993)
  95. Ratner A. J., Transplant. Proc., 24, 583 (1992)
  96. Reding R., Transplant. Immunol (1994)
  97. Ross J. R., Transplantation, 55, 1144 (1993)
  98. Sandrin M. S., Proc. Natl. Acad Sci. USA, 90, 11391 (1993)
  99. Schwartz Ronald H., Acquisition of immunologic self-tolerance, 10.1016/0092-8674(89)90044-5
  100. Sharabi Y., Specific tolerance induction across a xenogeneic barrier: production of mixed rat/mouse lymphohematopoietic chimeras using a nonlethal preparative regimen, 10.1084/jem.172.1.195
  101. Soares M., Transplantation, 56, 1427 (1993)
  102. Soares M., Transplant. Proc., 24, 451 (1992)
  103. Soares M., Transplantation, 57, 1 (1994)
  104. Soares M., Transplant. Proc. (1994)
  105. Soares M., Xenotransplantation (1994)
  106. Tamura K., Immunology, 79, 587 (1993)
  107. Thornton B. P, J. Immunol., 152, 1727 (1994)
  108. Tong T. S. K., J. Cell Biochem., 242 (1993)
  109. Tsubata Takeshi, Wu Jing, Honjo Tasuku, B-cell apoptosis induced by antigen receptor crosslinking is blocked by a T-cell signal through CD40, 10.1038/364645a0
  110. Turman M. A., Transplantation, 52, 710 (1991)
  111. P. J. Tuso, D. V Cramer, Y. D Middleton, M. Kearns-Jonker, C Yasunaga, C. A. Cosenza, W. C Davis, G. D. Wu, and L. Makowka (1993 ) Pig aortic endothelial cell antigens recognized by human IgM natural antibodies.
  112. W. J Werf, M. L. Blakely, W. W Hancock, M. Soares, D. Latinne, H. Bazin, and F. H. Bach (1993 ) Sustained suppression of xenoreactive natural antibodies in rats: anti-IgM monoclonal antibody treatment plus anti-B cell immunosuppressants . Second International Congress on Xenotransplantation. Cambridge, England. Book of Abstracts, p.60 .
  113. Vanhove B., Transplantation, 56, 1251 (1993)
  114. Vanhove B., Immunology, 79, 580 (1993)
  115. H. A. Vaughan, P. L. Dabkowski, and I. F. C. McKenzie (1993 ) Biochemical analysis of the major pig xenoantigens recognized by humans . Second International Congress on Xenotransplantation. Cambridge, England. Book of Abstracts, p.52 .
Bibliographic reference Latinne, Dominique ; Soares, Miguel ; Havaux, Xavier ; Cormont, Françoise ; Lesnikoski, Beth ; et. al. Depletion of IgM xenoreactive natural antibodies by injection of anti-mu monoclonal antibodies. In: Immunological Reviews, no. 141, p. 95-125 (1994)
Permanent URL http://hdl.handle.net/2078.1/13418