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Probe ligation and real-time detection of KPC, OXA-48, VIM, IMP, and NDM carbapenemase genes

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Cuzon, G. Naas, T. Bogaerts, Pierre [UCL] Glupczynski, Gerald [UCL] Nordmann, P.

The Check-MDR Carba test (Check-Points, Wageningen, Netherlands), which is based on specific molecular recognition of blaNDM, blaKPC, blaOXA-48, blaVIM, and blaIMP genes by DNA probe ligation and real-time PCR detection, was evaluated on 183 well-characterized Gram-negative rods. Representatives of the 5 gene families were accurately identified (specificities and sensitivities of 100%) within 4.5 hours. This test may be helpful to differentiate carbapenem resistance mediated by carbapenemases from those involving other mechanisms. © 2013 Elsevier Inc. All rights reserved.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2013
Language Anglais
Journal information "Diagnostic Microbiology and Infectious Disease" - Vol. 76, p. 502-505 (2013)
Peer reviewed yes
issn 0732-8893
Publication status Publié
Affiliations UCL - SSS/IREC/MBLG - Pôle de Microbiologie médicale
UCL - (MGD) Laboratoire de biologie clinique
Keywords Carbapenem resistance ; Detection ; Real-time PCR
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Bibliographic reference Cuzon, G. ; Naas, T. ; Bogaerts, Pierre ; Glupczynski, Gerald ; Nordmann, P.. Probe ligation and real-time detection of KPC, OXA-48, VIM, IMP, and NDM carbapenemase genes. In: Diagnostic Microbiology and Infectious Disease, Vol. 76, p. 502-505 (2013)
Permanent URL http://hdl.handle.net/2078.1/132631