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NeuroD1 induces terminal neuronal differentiation in olfactory neurogenesis

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Boutin, Camille [UCL] Hardt, Olaf [Institut de Biologie du Développement de Marseille Luminy] de Chevigny, Antoine [Institut de Biologie du Développement de Marseille Luminy] Coré, Nathalie [Institut de Biologie du Développement de Marseille Luminy] Goebbels, Sandra [Institut de Biologie du Développement de Marseille Luminy] Cremer, Harold [Institut de Biologie du Développement de Marseille Luminy]

After their generation and specification in periventricular regions, neuronal precursors maintain an immature and migratory state until their arrival in the respective target structures. Only here are terminal differentiation and synaptic integration induced. Although the molecular control of neuronal specification has started to be elucidated, little is known about the factors that control the latest maturation steps. We aimed at identifying factors that induce terminal differentiation during postnatal and adult neurogenesis, thereby focusing on the generation of periglomerular interneurons in the olfactory bulb. We isolated neuronal precursors and mature neurons from the periglomerular neuron lineage and analyzed their gene expression by microarray. We found that expression of the bHLH transcription factor NeuroD1 strikingly coincides with terminal differentiation. Using brain electroporation, we show that overexpression of NeuroD1 in the periventricular region in vivo leads to the rapid appearance of cells with morphological and molecular characteristics of mature neurons in the subventricular zone and rostral migratory stream. Conversely, shRNA-induced knockdown of NeuroD1 inhibits terminal neuronal differentiation. Thus, expression of a single transcription factor is sufficient to induce neuronal differentiation of neural progenitors in regions that normally do not show addition of new neurons. These results suggest a considerable potential of NeuroD1 for use in cell-therapeutic approaches in the nervous system.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 2010
Language Anglais
Journal information "Proceedings of the National academy of sciences of the United States of America" - Vol. 107, no.3, p. 1201-6 (19 janvier 2010)
Peer reviewed yes
Publisher National academy of sciences ((United States) [S.l.])
issn 0027-8424
e-issn 1091-6490
Publication status Publié
Affiliation UCL - SSS/IONS/CEMO - Pôle Cellulaire et moléculaire
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Bibliographic reference Boutin, Camille ; Hardt, Olaf ; de Chevigny, Antoine ; Coré, Nathalie ; Goebbels, Sandra ; et. al. NeuroD1 induces terminal neuronal differentiation in olfactory neurogenesis. In: Proceedings of the National academy of sciences of the United States of America, Vol. 107, no.3, p. 1201-6 (19 janvier 2010)
Permanent URL http://hdl.handle.net/2078.1/126498