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YopT, a new Yersinia Yop effector protein, affects the cytoskeleton of host cells.

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Iriarte, M. Cornelis, G R

Extracellular Yersinia disarm the immune system of their host by injecting effector Yop proteins into the cytosol of target cells. Five effectors have been described: YopE, YopH, YpkA/YopO, YopP and YopM. Delivery of these effectors by Yersinia adhering at the cell surface requires other Yops (translocators) including YopB. Effector and translocator Yops are secreted by the type III Ysc secretion apparatus, and some Yops also need a specific cytosolic chaperone, called Syc. In this paper, we describe a new Yop, which we have called YopT (35.5kDa). Its secretion required an intact Ysc apparatus and SycT (15.0kDa, pl4.4), a new chaperone resembling SycE. Infection of macrophages with a Yersinia, producing a hybrid YopT-adenylate cyclase, led to the accumulation of intracellular cAMP, indicating that YopT is delivered into the cytosol of eukaryotic cells. Infection of HeLa cells with a mutant strain devoid of the five known Yop effectors (deltaHOPEM strain) but producing YopT resulted in the alteration of the cell cytoskeleton and the disruption of the actin filament structure. This cytotoxic effect was caused by YopT and dependent on YopB. YopT is thus a new effector Yop and a new bacterial toxin affecting the cytoskeleton of eukaryotic cells.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Publication date 1998
Journal information "Molecular microbiology" - Vol. 29, no. 3, p. 915-29 (1998)
Peer reviewed yes
issn 0950-382X
Publication status Publié
Affiliation UCL
MESH Subject Amino Acid Sequence ; Animals ; Bacterial Proteins - genetics - metabolism ; Base Sequence ; Cysteine Endopeptidases ; Cytoskeleton ; Cytotoxins - genetics - metabolism ; DNA, Bacterial ; Eukaryotic Cells ; Hela Cells ; Humans ; Mice ; Molecular Sequence Data ; Open Reading Frames ; Operon ; Peyer's Patches ; Transcription, Genetic ; Yersinia enterocolitica - genetics - metabolism - physiology
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  1. Aktories K., J Clin Invest, 29, 827 (1997)
  2. Allaoui A., J Bacteriol, 29, 4534 (1994)
  3. Allaoui A., Mol Microbiol, 29, 343 (1995)
  4. Altschul S, Basic Local Alignment Search Tool, 10.1006/jmbi.1990.9999
  5. Andersson K., Mol Microbiol, 29, 1057 (1996)
  6. Arricau N., Hermant D., Waxin H., Popoff M.Y., Molecular characterization of the Salmonella typhi StpA protein that is related to both Yersinia YopE cytotoxin and YopH tyrosine phosphatase, 10.1016/s0923-2508(97)81896-7
  7. Bergman T., J Bacteriol, 29, 2619 (1994)
  8. Black D. S., Identification of p130Cas as a substrate of Yersinia YopH (Yop51), a bacterial protein tyrosine phosphatase that translocates into mammalian cells and targets focal adhesions, 10.1093/emboj/16.10.2730
  9. Bliska J.B., Infect Immun, 29, 3914 (1993)
  10. Boland A., Infect Immun, 29, 1878 (1998)
  11. Boland A., EMBO J, 29, 5191 (1996)
  12. Boyd A.P., Mol Microbiol, 29, 425 (1998)
  13. Cheng L.W., Mol Microbiol, 29, 757 (1997)
  14. China B., Mol Microbiol, 29, 1585 (1990)
  15. Cole S.P., Mol Microbiol, 29, 1895 (1992)
  16. Cole S. P., Guiney D. G., Corbeil L. B., Molecular analysis of a gene encoding a serum-resistance-associated 76 kDa surface antigen of Haemophilus somnus, 10.1099/00221287-139-9-2135
  17. Corbeil L.B., Infect Immun, 29, 4250 (1997)
  18. Cornelis G., Abraham E. P.,  -Lactamases from Yersinia enterocolitica, 10.1099/00221287-87-2-273
  19. Cornelis G., Colson C., Restriction of DNA in Yersinia enterocolitica Detected Recipient Ability for a Derepressed R Factor from Escherichia coli, 10.1099/00221287-87-2-285
  20. Cornelis G.R., Mol Microbiol, 29, 861 (1997)
  21. Cornelis G., J Bacteriol, 29, 254 (1989)
  22. Eisenberg D., J Mol Biol, 29, 125 (1984)
  23. Fallman M., Infect Immun, 29, 3117 (1995)
  24. Fields K.A., J Bacteriol, 29, 569 (1994)
  25. Finck-Barbancon V., Mol Microbiol, 29, 547 (1997)
  26. Forsberg A., Mol Microbiol, 29, 121 (1988)
  27. Forsberg A., Trends Microbiol, 29, 14 (1994)
  28. Frithz-Lindsten E., Mol Microbiol, 29, 635 (1995)
  29. Frithz-Lindsten E., Mol Microbiol, 29, 1125 (1997)
  30. Fu Y., Mol Microbiol, 29, 359 (1998)
  31. Galyov E.E., Nature, 29, 730 (1993)
  32. Gish W., Nature Genet, 29, 266 (1993)
  33. Hakansson S., Mol Microbiol, 29, 593 (1996)
  34. Hartland E.L., Infect Immun, 29, 4445 (1994)
  35. Hauser A.R., Mol Microbiol, 29, 807 (1998)
  36. Iriarte M., J Bacteriol, 29, 2299 (1995)
  37. Iriarte Maite, Sory Marie-Paule, Boland Anne, Boyd Aoife P., Mills Scott D., Lambermont Isabelle, Cornelis Guy R., TyeA, a protein involved in control of Yop release and in translocation of Yersinia Yop effectors, 10.1093/emboj/17.7.1907
  38. Jerse A.E., Proc Natl Acad Sci USA, 29, 7839 (1990)
  39. Kaniga Koné, Delor Isabelle, Cornelis Guy R., A wide-host-range suicide vector for improving reverse genetics in Gram-negative bacteria: inactivation of the blaA gene of Yersinia enterocolitica, 10.1016/0378-1119(91)90599-7
  40. Lambert de Rouvroit C., Mol Microbiol, 29, 395 (1992)
  41. Michiels T., J Bacteriol, 29, 4994 (1991)
  42. Miller V.L., J Bacteriol, 29, 2575 (1988)
  43. Mills S. D., Boland A., Sory M.-P., van der Smissen P., Kerbourch C., Finlay B. B., Cornelis G. R., Yersinia enterocolitica induces apoptosis in macrophages by a process requiring functional type III secretion and translocation mechanisms and involving YopP, presumably acting as an effector protein, 10.1073/pnas.94.23.12638
  44. Monack D. M., Mecsas J., Ghori N., Falkow S., Yersinia signals macrophages to undergo apoptosis and YopJ is necessary for this cell death, 10.1073/pnas.94.19.10385
  45. Neyt C, J Bacteriol, 29, 612 (1997)
  46. Palmer L.E., Mol Microbiol, 29, 953 (1998)
  47. Pearson W.R., Proc Natl Acad Sci USA, 29, 2444 (1988)
  48. Persson C., Mol Microbiol, 29, 135 (1995)
  49. Persson Cathrine, Carballeira Nivia, Wolf-Watz Hans, Fällman Maria, The PTPase YopH inhibits uptake ofYersinia, tyrosine phosphorylation of p130Casand FAK, and the associated accumulation of these proteins in peripheral focal adhesions, 10.1093/emboj/16.9.2307
  50. Plano G.V., J Bacteriol, 29, 7293 (1991)
  51. 51A.M. Popowicz, and P.F. Dash (1989 )Sigseq Version 1.0. Prediction of signal sequence cleavage sites based on the method of G. von Heijne. New York: The Rockefeller University.
  52. Portnoy D.A., J Bacteriol, 29, 877 (1981)
  53. Ridley A.J., Cell, 29, 389 (1992)
  54. Rosqvist R., Mol Microbiol, 29, 657 (1990)
  55. Rosqvist R., Infect Immun, 29, 4562 (1991)
  56. Rosqvist R., EMBO J, 29, 964 (1994)
  57. Ruckdeschel K., Infect Immun, 29, 724 (1996)
  58. Sarker M., Infect Immun, 29, 2976 (1998)
  59. Sarker M., J Bacteriol, 29, 1207 (1998)
  60. Sory M.P., Mol Microbiol, 29, 583 (1994)
  61. Sory M.P., Infect Immun, 29, 3830 (1992)
  62. Sory M.P., Proc Natl Acad Sci USA, 29, 11998 (1995)
  63. Tabor S., Proc Natl Acad Sci USA, 29, 1074 (1985)
  64. Wattiau P., Mol Microbiol, 29, 123 (1993)
  65. Wattiau P., Proc Natl Acad Sci USA, 29, 10493 (1994)
  66. Wattiau P., Mol Microbiol, 29, 255 (1996)
  67. Woestyn S., J Bacteriol, 29, 1561 (1994)
  68. Woestyn S., Mol Microbiol, 29, 1261 (1996)
  69. Yahr T.L., J Bacteriol, 29, 1169 (1995)
Bibliographic reference Iriarte, M. ; Cornelis, G R. YopT, a new Yersinia Yop effector protein, affects the cytoskeleton of host cells.. In: Molecular microbiology, Vol. 29, no. 3, p. 915-29 (1998)
Permanent URL http://hdl.handle.net/2078.1/12463