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Identification, characterization and mapping of differentially expressed genes in a winter wheat cultivar (Centenaire) resistant to Fusarium graminearum infection.

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Muhovski, Yordan [Life Sciences Department, Walloon Agricultural Research Centre, Gembloux (Belgium)c] Batoko, Henri [UCL] Jacquemin, Jean-Marie [Life Sciences Department, Walloon Agricultural Research Centre, Gembloux (Belgium)c]

Fusarium head blight (FHB), predominantly caused by Fusarium graminearum, is a destructive disease that poses a serious threat to wheat (Triticum aestivum L.) production around the world. A suppression subtractive hybridization (SSH) cDNA library was constructed from F. graminearum infected spikes of a resistant Belgian winter wheat variety Centenaire, exhibiting Type II resistance to FHB. Forty-three differentially expressed transcripts were identified and classified in different categories according to their predicted function, including proteins involved in defense response, signaling, transport of molecules, metabolism and proteins with unknown function. Time-course gene expression analysis between the FHB resistant genotype Centenaire and the susceptible genotype Robigus was carried out on twelve selected genes in order to validate the SSH screening. Real-time quantitative polymerase chain reaction showed that the selected transcripts were differentially expressed between the resistant and the susceptible genotype at three-time points (24, 48 and 72 h) after inoculation with the pathogen, and mostly, the transcripts accumulation rates were higher in the FHB-resistant as compared to the susceptible one. Thirty identified differentially expressed loci were mapped on the corresponding wheat chromosomes either by in silico analysis or by PCR-based mapping strategy, and fifteen of these loci were located within or nearby chromosomal regions known to have quantitative trait loci for FHB resistance in winter wheat cultivars. This work emphasizes the differential gene expression between the FHB-resistant winter wheat Centenaire and the susceptible Robigus and highlights the putative genes and mechanism involved in the disease resistance reaction.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2012
Language Anglais
Journal information "Molecular Biology Reports : an international journal on molecular and cellular biology" - Vol. 39, no.10, p. 9583-600 (2012)
Peer reviewed yes
Publisher Springer Netherlands ((Netherlands) Dordrecht)
issn 0301-4851
e-issn 1573-4978
Publication status Publié
Affiliations UCL - SST/ISV - Institut des sciences de la vie
UCL - SST/LIBST - Louvain Institute of Biomolecular Science and Technology
MESH Subject Chromosome Mapping ; Plant Proteins ; Quantitative Trait Loci ; Real-Time Polymerase Chain Reaction ; Sequence Analysis, DNA ; Transcriptome ; Triticum ; Chromosomes, Plant ; Disease Resistance ; Expressed Sequence Tags ; Fusarium ; Gene Expression ; Gene Expression Regulation, Plant ; Genes, Plant ; Plant Diseases
Keywords 3100
Links
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Bibliographic reference Muhovski, Yordan ; Batoko, Henri ; Jacquemin, Jean-Marie. Identification, characterization and mapping of differentially expressed genes in a winter wheat cultivar (Centenaire) resistant to Fusarium graminearum infection.. In: Molecular Biology Reports : an international journal on molecular and cellular biology, Vol. 39, no.10, p. 9583-600 (2012)
Permanent URL http://hdl.handle.net/2078.1/124605