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Surface modification of polypropylene nonwovens with LDV peptidomimetics and their application in the leukodepletion of blood products

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Gérard, E. [UCL] Bessy, E. Hénard, G. Verpoort, T. Marchand-Brynaert, Jacqueline [UCL]

For clinical indications and according to European standards, a depletion of the leukocytes from whole blood has to be realized before transfusion to a patient. In this study, the surface of a layer of meltblown oxygen-plasma treated polypropylene nonwoven (O 2-PP), making part of the composition of leukodepletion filters, was modified with bioactive molecules to enhance the adhesion of leukocytes. These synthetic small molecules (called peptidomimetics) mimic the "Leu-Asp-Val" (LDV) tripeptide sequence recognized by the α 4β 1 integrin, a receptor expressed on leukocytes. Their activity, as ligands of the α 4β 1 integrin, was confirmed through cell adhesion assays. The peptidomimetics were covalently immobilized on O 2-PP nonwoven via activation of the hydroxyl- and carboxyl-functions displayed on the polymer surface with trifluoro-triazine reagent, or were simply deposited on the O 2-PP surface. The treated materials were characterized by X-ray photoelectron spectroscopy, wettability, and morphological analyses, before and after steam sterilization. Experimental filters composed of 10 layers of O 2-PP nonwovens and a last layer modified with the peptidomimetics were evaluated, using whole blood filtration assays, for their leukodepletion efficiency, the recovery of red cells and platelets and the waste of blood, with an objective to design new filters fulfilling practical and medical criteria. © 2012 Wiley Periodicals, Inc.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2012
Language Anglais
Journal information "Journal of Biomedical Materials Research - Part B Applied Biomaterials" - Vol. 100 B, no.6, p. 1513-1523 (2012)
Peer reviewed yes
Publisher Wiley
issn 1552-4973
Publication status Publié
Affiliation UCL - SST/IMCN/MOST - Molecules, Solids and Reactivity
Keywords Α ; Blood products ; Cell-adhesion assays ; European Standards ; Integrins ; Leukocyte depletion ; Meltblown ; Morphological analysis ; Non-woven ; Nonwovens ; Oxygen plasmas ; 4β ; Peptidomimetics ; Polymer surfaces ; Polypropylene nonwovens ; PP nonwoven ; Red cells ; Small molecules ; Steam sterilization ; Tripeptide ; Whole blood ; Blood ; 1 integrins ; Cell adhesion ; Molecular biology ; Nonwoven fabrics ; Photoelectrons ; Surface treatment ; Surfaces ; Weaving ; X ray photoelectron spectroscopy ; Polypropylenes ; Peptidomimetic agent ; LDV peptidomimetics ; Polypropylene ; Very late activation antigen 4 ; Article ; Blood filter ; Blood filtration ; Cell adhesion ; Chemical modification ; Chemical structure ; Leukocyte ; Leukodepletion filter ; Leukocyte depletion ; Wettability ; X ray photoelectron spectroscopy ; Blood Platelets ; Cell Adhesion ; Erythrocytes ; Female ; Humans ; Leukapheresis ; Leukocytes ; Male ; Polypropylene nonwovens ; Membranes, Artificial ; Polypropylenes ; Surface modification ; As-ligands ; Bioactive molecules
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Bibliographic reference Gérard, E. ; Bessy, E. ; Hénard, G. ; Verpoort, T. ; Marchand-Brynaert, Jacqueline. Surface modification of polypropylene nonwovens with LDV peptidomimetics and their application in the leukodepletion of blood products. In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, Vol. 100 B, no.6, p. 1513-1523 (2012)
Permanent URL http://hdl.handle.net/2078.1/124527