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Role of protein-phosphorylation events in the anoxia signal-transduction pathway leading to the inhibition of total protein synthesis in isolated hepatocytes.

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Tinton, S Tran-Nguyen, Q N Buc Calderon, Pedro [UCL]

Incubation of isolated hepatocytes under N2/CO2 (no O2) produced a rapid and strong inhibition of overall polypeptide biosynthesis, which was neither related to cell death nor to the appearance of specific stress proteins. Treatment of the cells with the tyrosine-kinase inhibitor genistein or with the serine/threonine-protein-kinase inhibitor H7 did not modify the impairment of protein synthesis induced by oxygen deprivation, indicating that such signal-transduction pathways are probably not involved in the anoxia-mediated effect. Okadaic acid (100 nM) and Na3VO4 (1 mM) reduced the incorporation of [14C]Leu into proteins of hepatocytes maintained under aerobic conditions (93.3 kPa O2). The effects of oxygen deprivation and okadaic acid were additive, whereas sodium vanadate did not enhance the impairment of protein synthesis induced by anoxia. This observation suggests that a common mechanism, involving the net phosphorylation of protein tyrosine residues, that is insensitive to genistein might participate in the negative control of the translation induced by oxygen deprivation. The effect of anoxia on the synthesis of proteins was fully and rapidly reversible upon the restoration of oxygen supply, thus indicating that hepatocytes are able to sense O2. Although high concentrations of cobalt chloride partially mimic the effect of oxygen deprivation on protein biosynthesis, the nature of such an oxygen sensor remains unknown, and appears unlikely to be a part of a classic haem protein.

Document type Article de périodique (Journal article) – In Vitro, Journal Article, Research Support, Non-U.S. Gov't
Publication date 1997
Journal information "European journal of biochemistry / FEBS" - Vol. 249, no. 1, p. 121-6 (1997)
Peer reviewed yes
issn 0014-2956
Publication status Publié
Affiliation UCL
MESH Subject Animals ; Cell Hypoxia - physiology ; Cell Survival ; Cobalt - pharmacology ; Enzyme Inhibitors - pharmacology ; Liver - cytology - metabolism ; Male ; Phosphoprotein Phosphatases - antagonists & inhibitors ; Phosphorylation ; Protein Biosynthesis ; Protein Kinase Inhibitors ; Proteins - metabolism ; Rats ; Rats, Wistar ; Signal Transduction - physiology
Links
  1. Hochachka P. W., Science, 231, 234 (1986)
  2. Krumschnabel G., Am. J. Physiol., 270, R614 (1996)
  3. Hochachka P. W., Buck L. T., Doll C. J., Land S. C., Unifying theory of hypoxia tolerance: molecular/metabolic defense and rescue mechanisms for surviving oxygen lack., 10.1073/pnas.93.18.9493
  4. Krumschnabel Gerhard, Schwarzbaum Pablo J., Wieser Wolfgang, Coupling of Energy Supply and Energy Demand in Isolated Goldfish Hepatocytes, 10.1086/physzool.67.2.30163857
  5. Kraggerud S. M., Anticancer Res., 15, 683 (1995)
  6. Land S. C., Am. J. Physiol., 265, R41 (1993)
  7. Land S. C., Proc. Natl Acad. Sci. USA, 92, 7505 (1995)
  8. Lefebvre V.H.L., Vansteenbrugge M., Beckers V., Roberfroid M., Buccalderon P., Adenine Nucleotides and Inhibition of Protein Synthesis in Isolated Hepatocytes Incubated under Different pO2 Levels, 10.1006/abbi.1993.1357
  9. Kwast Kurt E., Hand Steven C., Acute Depression of Mitochondrial Protein Synthesis during Anoxia : CONTRIBUTIONS OF OXYGEN SENSING, MATRIX ACIDIFICATION, AND REDOX STATE, 10.1074/jbc.271.13.7313
  10. Vreugdenhil P. K., Hepatology, 16, 241 (1992)
  11. Nakagohri T., Transplant. Proc., 21, 2292 (1989)
  12. Goldberg M. A., Science, 242, 1412 (1988)
  13. Tan C. C., Am. J. Physiol., 263, F474 (1992)
  14. Marti H. H., Eur. J. Physiol,, 429, 216 (1994)
  15. Semenza G. L., J. Biol. Chem., 269, 23757 (1994)
  16. Wenger R., Blood, 87, 756 (1996)
  17. Shweiki Dorit, Itin Ahuva, Soffer Dov, Keshet Eli, Vascular endothelial growth factor induced by hypoxia may mediate hypoxia-initiated angiogenesis, 10.1038/359843a0
  18. Wang G. L., J. Biol. Chem., 268, 21513 (1993)
  19. Rupec R. A., Eur. J. Biochem., 234, 632 (1995)
  20. Hershey J., Translational Control In Mammalian Cells, 10.1146/annurev.biochem.60.1.717
  21. Kietzmann T., Schmidt H., Unthanfechner K., Probst I., Jungermann K., A Ferro-Heme Protein Senses Oxygen Levels, Which Modulate the Glucagon-Dependent Activation of the Phosphoenolpyruvate Carboxykinase Gene in Rat Hepatocyte Cultures, 10.1006/bbrc.1993.2115
  22. Acker H., Ann. N. Y. Acad. Sci., 718, 3 (1994)
  23. Bunn F. H., Physiol. Rev., 76, 839 (1996)
  24. Tinton Sandrine A., Lefebvre Véronique H., Cousin Olivier C., Buc-Calderon Pedro M., Cytolytic effects and biochemical changes induced by extracellular ATP to isolated hepatocytes, 10.1016/0167-4889(93)90169-p
  25. Krack G., Goethals F., Deboyser D., Roberfroid M., Interference of chemicals with glycogen metabolism in isolated hepatocytes, 10.1016/0300-483x(80)90066-9
  26. Seglen Per O., Incorporation of radioactive amino acids into protein in isolated rat hepatocytes, 10.1016/0005-2787(76)90313-0
  27. Lowry H. O., J. Biol. Chem., 193, 265 (1951)
  28. Laemmli U. K., Nature, 227, 680 (1970)
  29. Rao P. U., Am. J. Physiol., 267, L271 (1994)
  30. Murphy BJ, Laderoute KR, Short SM, Sutherland RM, The identification of heme oxygenase as a major hypoxic stress protein in Chinese hamster ovary cells, 10.1038/bjc.1991.241
  31. Qi Y., J. Neurol., 63, 1485 (1994)
  32. Cairo G., Hepatology, 5, 357 (1985)
  33. Schiaffonati L., Transplantation, 55, 977 (1993)
  34. Nairn A. C., J. Biol. Chem., 262, 17299 (1987)
  35. Ochoa Severo, de Haro Cesar, Regulation of Protein Synthesis in Eukaryotes, 10.1146/annurev.bi.48.070179.003001
  36. Menaya J, Parrilla R, Ayuso M S, Action of phenylephrine on protein synthesis in liver cells, 10.1042/bj2480903
  37. Curran R. D., FASEB J., 5, 2085 (1991)
  38. Hu B. R., J. Neurosci., 13, 1830 (1993)
  39. Eckardt K.-U., Pflugers Arch. Eur. J. Physiol., 426, 21 (1994)
  40. Wang G.L., Jiang B.H., Semenza G.L., Effect of Protein Kinase and Phosphatase Inhibitors on Expression of Hypoxia Inducible Factor 1, 10.1006/bbrc.1995.2674
  41. BODI I, Cell-specificity and signaling pathway of endothelin-1 gene regulation by hypoxia, 10.1016/0008-6363(95)00164-6
  42. Claeyssens S, Francois A, Chedeville A, Lavoinne A, Microcystin-LR induced an inhibition of protein synthesis in isolated rat hepatocytes, 10.1042/bj3060693
  43. Redpath N T, Proud C G, The tumour promoter okadaic acid inhibits reticulocyte-lysate protein synthesis by increasing the net phosphorylation of elongation factor 2, 10.1042/bj2620069
  44. Gilles-Gonzalez Marie A., Ditta Gary S., Helinski Donald R., A haemoprotein with kinase activity encoded by the oxygen sensor of Rhizobium meliloti , 10.1038/350170a0
  45. Göpfert Thea, Gess Bernhard, Eckardt Kai-Uwe, Kurtz Armin, Hypoxia signalling in the control of erythropoietin gene expression in rat hepatocytes, 10.1002/(sici)1097-4652(199608)168:2<354::aid-jcp14>3.0.co;2-3
  46. Wang G. L., Proc. Natl Acad. Sci. USA, 92, 5510 (1995)
  47. Maltepe Emin, Schmidt Jennifer V., Baunoch David, Bradfield Christopher A., Simon M. Celeste, Abnormal angiogenesis and responses to glucose and oxygen deprivation in mice lacking the protein ARNT, 10.1038/386403a0
  48. Lefebvre V., Hepatology, 20, 1567 (1994)
Bibliographic reference Tinton, S ; Tran-Nguyen, Q N ; Buc Calderon, Pedro. Role of protein-phosphorylation events in the anoxia signal-transduction pathway leading to the inhibition of total protein synthesis in isolated hepatocytes.. In: European journal of biochemistry / FEBS, Vol. 249, no. 1, p. 121-6 (1997)
Permanent URL http://hdl.handle.net/2078.1/12379