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Antibody-functionalized polymer-coated gold nanoparticles targeting cancer cells: an in vitro and in vivo study

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Marega, Riccardo Karmani, Linda [UCL] Flamant, Lionel Nageswaran, Praveen Ganesh Valembois, Vanessa Feron, Olivier [UCL] Vander Borght, Thierry [UCL] Gallez, Bernard [UCL] Bonifazi, Davide

Gold nanoparticles ([similar]5 nm) coated with plasma-polymerized allylamine were produced through plasma vapor deposition and bioconjugated with a monoclonal antibody targeting the epidermal growth factor receptor. The resulting nanoconjugates displayed an antibody loading of about 1.7 nmol mg−1 and efficiently target epidermal growth factor receptor overexpressing cell lines, as ascertained by ELISA and Western blot assays. The in vitro targeting properties were also confirmed in vivo, where a similar biodistribution profile of what was experienced for the unconjugated antibody was observed. Thanks to the possibility of doping the gold nanoparticles with radionuclides during plasma vapor deposition, the proposed functionalization strategy represents a very suitable platform for the in vivo cancer targeting with nanosized multifunctional particles.

Document type Article de périodique (Journal article) – Article de recherche
Publication date 2012
Language Anglais
Journal information "Journal of Materials Chemistry" - Vol. 22, no. 39, p. 21305-21312 (2012)
Peer reviewed yes
Publisher Royal Society of Chemistry ((United Kingdom) Cambridge)
issn 0959-9428
e-issn 1364-5501
Publication status Publié
Affiliations UCL - (MGD) Service de médecine nucléaire
UCL - SSS/IREC/MONT - Pôle Mont Godinne
UCL - SSS/IREC/FATH - Pôle de Pharmacologie et thérapeutique
UCL - SSS/LDRI - Louvain Drug Research Institute
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Bibliographic reference Marega, Riccardo ; Karmani, Linda ; Flamant, Lionel ; Nageswaran, Praveen Ganesh ; Valembois, Vanessa ; et. al. Antibody-functionalized polymer-coated gold nanoparticles targeting cancer cells: an in vitro and in vivo study. In: Journal of Materials Chemistry, Vol. 22, no. 39, p. 21305-21312 (2012)
Permanent URL http://hdl.handle.net/2078.1/123307