User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

Mitochondrial DNA polymerases from yeast to man: a new family of polymerases.

Primary tabs

Lecrenier, N van der Bruggen, Pierre [UCL] Foury, Françoise [UCL]

We report the sequence of a 4.5-kb cDNA clone isolated from a human melanoma library which bears high amino acid sequence identity to the yeast mitochondrial (mt) DNA polymerase (Mip1p). This cDNA contains a 3720-bp open reading frame encoding a predicted 140-kDa polypeptide that is 43% identical to Mip1p. The N-terminal part of the sequence contains a 13 glutamine stretch encoded by a CAG trinucleotide repeat which is not found in the other DNA polymerases gamma (Pol gamma). Multiple amino acid sequence alignments with Pol gamma from Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris, Drosophila melanogaster, Xenopus laevis and Mus musculus show that these DNA polymerases form a family strongly conserved from yeast to man and are only loosely related to the Family A DNA polymerases.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 1997
Journal information "Gene" - Vol. 185, no. 1, p. 147-52 (1997)
Peer reviewed yes
issn 0378-1119
Publication status Publié
Affiliation UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries
MESH Subject Amino Acid Sequence ; Animals ; Chromosome Mapping ; Cloning, Molecular ; DNA, Complementary - genetics ; DNA-Directed DNA Polymerase - classification - genetics ; Drosophila melanogaster ; Exonucleases - genetics ; Gene Library ; Humans ; Melanoma - genetics ; Mice ; Mitochondria - genetics ; Molecular Sequence Data ; Phylogeny ; Pichia ; Repetitive Sequences, Nucleic Acid ; Saccharomyces cerevisiae ; Schizosaccharomyces ; Sequence Alignment ; Sequence Homology, Amino Acid ; Xenopus laevis
Links
Bibliographic reference Lecrenier, N ; van der Bruggen, Pierre ; Foury, Françoise. Mitochondrial DNA polymerases from yeast to man: a new family of polymerases.. In: Gene, Vol. 185, no. 1, p. 147-52 (1997)
Permanent URL http://hdl.handle.net/2078.1/12293