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Trypanosomes contain two highly different isoforms of peroxin PEX13 involved in glycosome biogenesis.

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Brennand, Ana Rigden, Daniel J Michels, Paulus [UCL]

We previously identified the peroxin PEX13 in Trypanosoma brucei. Although lacking some features considered typical of PEX13s, it appeared functional in the biogenesis of glycosomes, the peroxisome-like organelles of trypanosomatids. Here we report the identification of a very different trypanosomatid PEX13, not containing the commonly encountered PEX13 SH3 domain but having other typical features. It is readily detected with the jackhmmer database search program, but not with PSI-BLAST. This is the first time different PEX13 isoforms are reported in a single organism. We show that this PEX13.2, like the PEX13.1 previously described, is associated with glycosomes and that its depletion by RNA interference affects the biogenesis of the organelles and viability of trypanosomes. The features considered typical of PEX13s are discussed.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2012
Language Anglais
Journal information "FEBS Letters" - Vol. 586, no. 13, p. 1765-1771 (2012)
Peer reviewed yes
Publisher Elsevier BV ((Netherlands) [S.l.])
issn 0014-5793
e-issn 1873-3468
Publication status Publié
Affiliation UCL - SSS/DDUV - Institut de Duve
MESH Subject Amino Acid Sequence ; Trypanosoma brucei brucei ; src Homology Domains ; Biogenesis ; Microbodies ; Molecular Sequence Data ; Peroxisomes ; Protein Isoforms ; Protozoan Proteins ; RNA Interference ; Sequence Alignment
Links
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Bibliographic reference Brennand, Ana ; Rigden, Daniel J ; Michels, Paulus. Trypanosomes contain two highly different isoforms of peroxin PEX13 involved in glycosome biogenesis.. In: FEBS Letters, Vol. 586, no. 13, p. 1765-1771 (2012)
Permanent URL http://hdl.handle.net/2078/122479