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Continuous increase of alcohol dehydrogenase activity along the liver plate in normal and cirrhotic human livers.

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Sokal, Etienne [UCL] Collette, E. Buts, Jean-Paul [UCL]

To determine the zonal distribution of alcohol dehydrogenase in normal and cirrhotic human livers, we measured activities of this enzyme by quantitative cytochemical analysis along the liver cell plate in liver specimens from 10 normal organ donors and from 7 children with extrahepatic biliary atresia cirrhosis. In normal human liver samples, a continuous increase in alcohol dehydrogenase activity was observed along the sinusoid from the periportal to the perivenular hepatocytes (mean extinction units from 16.2 +/- 10.0 to 58.0 +/- 14.8). A similar observation was made in cirrhotic nodules, with activity increasing continuously from nodule periphery to center (7.6 +/- 4.1 to 44.9 +/- 13.3). This study demonstrates a heterogeneous pattern of alcohol dehydrogenase distribution along the sinusoid in normal human liver specimens. In addition, demonstration of this heterogeneity in human cirrhosis suggests that the cirrhotic liver is able to maintain a parenchymal functional organization, with persistence of metabolic zonation.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Publication date 1993
Journal information "Hepatology (Baltimore, Md.)" - Vol. 17, no. 2, p. 202-5 (1993)
Peer reviewed yes
issn 0270-9139
Publication status Publié
Affiliation UCL - MD/GYPE - Département de gynécologie, d'obstétrique et de pédiatrie
MESH Subject Adolescent ; Adult ; Alcohol Dehydrogenase - metabolism ; Biliary Atresia - complications ; Child ; Female ; Histocytochemistry ; Humans ; Liver - enzymology ; Liver Cirrhosis - complications - enzymology - pathology ; Liver Cirrhosis, Biliary - complications ; Male ; Reference Values
Links
  1. Vallee, Isozymes Curr Top Biol Med Res, 8, 219 (1983)
  2. Kato, HEPATOLOGY, 12, 66 (1990)
  3. Yamauchi, HEPATOLOGY, 8, 243 (1988)
  4. Mally, Biol Chem, 388, 315 (1987)
  5. Vaananen, HEPATOLOGY, 5, 862 (1984)
  6. Bengtsson, Eur J Biochem, 118, 591 (1981)
  7. Greenberger, Lab Invest, 14, 264 (1965)
  8. Gumucio, Am J Physiol, 234, c102 (1978)
  9. Morrison, J Lab Clin Med, 70, 116 (1967)
  10. Buehler, Am J Pathol, 108, 89 (1982)
  11. Sokal, J Hepatol, 9, 42 (1989)
  12. Otte, Ann Surg, 211, 146 (1989)
  13. , Practical histochemistry. London: John Wiley & Sons, 1973: 177–193.
  14. Wimmer, Histochemistry, 64, 23 (1979)
  15. Altman, Ciba Found Symp, 73, 81 (1979)
  16. Van Noorden, Histochem J, 17, 1111 (1985)
  17. , Principle of oxidoreductase histochemistry, In: , eds. Histochemistry, Vol 3, Enzyme histochemistry, 4th ed. London: Churchill Livingstone, 1992: 1–71.
  18. Goldstein, Histochemistry, 57, 107 (1978)
  19. Gumucio, HEPATOLOGY, 9, 154 (1989)
  20. Jungermann, Physiol Rev, 69, 708 (1989)
  21. Sokal Etienne M., Trivedi Premila, Portmann Bernard, Mowat Alex P., Adaptative changes of metabolic zonation during the development of cirrhosis in growing rats, 10.1016/0016-5085(90)90969-8
  22. , . Dehydrogenases involved in steroid and prostaglandin metabolism. In: , eds. Histochemistry, Vol 3, Enzyme Histochemistry, 4th ed. London: Churchill Livingstone, 1992: 73–93.
  23. Lieberman, Gastroenterology, 44, 261 (1963)
  24. Asada, Gastroenterology, 45, 67 (1963)
  25. Smith, Ann Hum Genet, 34, 251 (1971)
  26. Sokal, Pediatr Res, 30, 286 (1991)
  27. Sokal, HEPATOLOGY, 15, 904 (1992)
Bibliographic reference Sokal, Etienne ; Collette, E. ; Buts, Jean-Paul. Continuous increase of alcohol dehydrogenase activity along the liver plate in normal and cirrhotic human livers.. In: Hepatology (Baltimore, Md.), Vol. 17, no. 2, p. 202-5 (1993)
Permanent URL http://hdl.handle.net/2078.1/12103