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In-depth proteome mining of cultured Catharanthus roseus cells identifies candidate proteins involved in the synthesis and transport of secondary metabolites.

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Champagne, Antoine [UCL] Rischer, Heiko [VTT Biotechnology (Finland)] Oksman-Caldentey, Kirsi-Marja [VTT Biotechnology (Finland)] Boutry, Marc [UCL]

Madagascar periwinkle (Catharanthus roseus) is the major source of terpenoid indole alkaloids, such as vinblastine or vincristine, used as natural drugs against various cancers. In this study, we have extensively analyzed the proteome of cultured C. roseus cells. Comparison of the proteomes of two independent cell lines with different terpenoid indole alkaloid metabolism by 2D-DIGE revealed 358 proteins that differed quantitatively by at least a twofold average ratio. Of these, 172 were identified by MS; most corresponded to housekeeping proteins. Less abundant proteins were identified by LC separation of tryptic peptides of proteins from one of the lines. We identified 1663 proteins, most of which are housekeeping proteins or involved in primary metabolism. However, 63 enzymes potentially involved in secondary metabolism were also identified, of which 22 are involved in terpenoid indole alkaloid biosynthesis and 16 are predicted transporters putatively involved in secondary metabolite transport. About 30% of the proteins identified have an unclear or unknown function, indicating important gaps in knowledge of plant metabolism. This study is an important step toward elucidating the proteome of C. roseus, which is critical for a better understanding of how this plant synthesizes terpenoid indole alkaloids.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès interdit
Publication date 2012
Language Anglais
Journal information "Proteomics" - Vol. 12, no.23-24, p. 3536–3547 (December 2012)
Peer reviewed yes
Publisher Wiley - V C H Verlag GmbH & Co. KGaA ((Germany) Weinheim)
issn 1615-9853
e-issn 1615-9861
Publication status Publié
Keywords Alkaloids ; Catharanthus roseus ; MALDI-MS/MS ; Plant proteomics ; Terpenes ; Terpenoid indole alkaloids
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Bibliographic reference Champagne, Antoine ; Rischer, Heiko ; Oksman-Caldentey, Kirsi-Marja ; Boutry, Marc. In-depth proteome mining of cultured Catharanthus roseus cells identifies candidate proteins involved in the synthesis and transport of secondary metabolites.. In: Proteomics, Vol. 12, no.23-24, p. 3536–3547 (December 2012)
Permanent URL http://hdl.handle.net/2078/120953