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Plasminogen activator activity and fertilizing ability of human spermatozoa.

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Lison, Dominique [UCL] Tas, S Gennart, J P Psalti, I. de Cooman, Serge [UCL] Lauwerys, Robert [UCL]

Mature spermatozoa contain a number of proteases that are supposed to contribute to their fertilizing ability. The present study was directed at plasminogen activator (PA), a protease that belongs to the group of serine proteases and converts the zymogen plasminogen to the active broad-spectrum protease plasmin. To investigate the possible role of PA in the fertilization process, we have measured sperm-bound PA activity in 63 patients included in an in-vitro fertilization (IVF) programme and assessed their relationship to standard semen parameters and the rate of fertilization. PA activity was correlated significantly with the sperm count, as well as with sperm motility and morphology. Using logistic regression analysis, specific PA (pmol pNA 10(-6) cells min-2) was found to significantly influence the probability of fertilization. Other significantly predictive factors were motility and the percentage of spermatozoa with normal morphology. The sperm concentration (10(6) cells ml-1) did not significantly affect the outcome of IVF. We suggest that sperm-bound PA is involved in the fertilization process and may represent a potential indicator of sperm fertilizing capacity.

Document type Article de périodique (Journal article) – Journal Article
Publication date 1993
Journal information "International journal of andrology" - Vol. 16, no. 3, p. 201-6 (1993)
Peer reviewed yes
issn 0105-6263
Publication status Publié
Affiliation UCL
MESH Subject Female ; Fertilization ; Fertilization in Vitro ; Humans ; Infertility - enzymology ; Male ; Plasminogen Activators - metabolism ; Semen - enzymology ; Spermatozoa - enzymology - physiology
Links
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Bibliographic reference Lison, Dominique ; Tas, S ; Gennart, J P ; Psalti, I. ; de Cooman, Serge ; et. al. Plasminogen activator activity and fertilizing ability of human spermatozoa.. In: International journal of andrology, Vol. 16, no. 3, p. 201-6 (1993)
Permanent URL http://hdl.handle.net/2078.1/12084