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Genetic analysis and overexpression of lipolytic activity in Bacillus subtilis.

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Dartois, V. Colson, Charles [UCL] Baulard, A Coppée, J Y

The previously cloned Bacillus subtilis lipase gene (lip) was mapped on the chromosome and used in the construction of a B. subtilis derivative totally devoid of any lip sequence. Homologous overexpression was performed in this strain by subcloning the lip open reading frame on a multicopy plasmid under the control of a strong gram-positive promoter. A 100-fold overproducing strain was obtained, which should facilitate purification of the secreted protein. Furthermore, the delta lip strain BCL1050 constitutes an ideal host for the cloning of heterologous lipase genes.

Document type Article de périodique (Journal article) – Journal Article
Access type Accès libre
Publication date 1994
Journal information "Applied and environmental microbiology" - Vol. 60, no. 5, p. 1670-3 (1994)
Peer reviewed yes
issn 0099-2240
Publication status Publié
Affiliation UCL
MESH Subject Alleles ; Bacillus subtilis - enzymology - genetics ; Chromosome Mapping ; Chromosomes, Bacterial ; Gene Expression ; Lipase - biosynthesis - genetics - metabolism ; Plasmids
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Bibliographic reference Dartois, V. ; Colson, Charles ; Baulard, A ; Coppée, J Y. Genetic analysis and overexpression of lipolytic activity in Bacillus subtilis.. In: Applied and environmental microbiology, Vol. 60, no. 5, p. 1670-3 (1994)
Permanent URL http://hdl.handle.net/2078.1/11978