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Heterologous expression of an active rat regulatory protein of glucokinase

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Detheux, Michel [UCL] Van Schaftingen, Emile [UCL]

The cDNA presumed to encode the rat liver regulatory protein of glucokinase has been expressed in Escherichia coli and a partially soluble protein has been obtained. This recombinant protein was partially purified and found to have the same apparent molecular mass as the regulatory protein purified from rat liver. Like the latter, it inhibited rat liver glucokinase competitively with respect to glucose and its effect was sensitive to fructose 6-phosphate and fructose 1-phosphate.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 1994
Journal information "FEBS Letters" - Vol. 355, no. 1, p. 27-29 (1994)
Publisher Elsevier BV
issn 0014-5793
e-issn 1873-3468
Publication status Publié
Affiliation UCL - MD/BICL - Département de biochimie et de biologie cellulaire
MESH Subject Animals ; Base Sequence ; Carrier Proteins ; Escherichia coli - genetics - metabolism ; Fructosephosphates - pharmacology ; Glucokinase - antagonists & inhibitors ; Glucose - metabolism ; Liver - chemistry - enzymology ; Molecular Sequence Data ; Molecular Weight ; Protein Biosynthesis ; Proteins - chemistry - isolation & purification - pharmacology ; Rats ; Recombinant Proteins - biosynthesis - chemistry - isolation & purification - pharmacology ; Solubility
Keywords Glucokinase ; Glycolysis ; Fructose 1-phosphate ; Fructose 6-phosphate
Links
  1. SCHAFTINGEN Emile, A protein from rat liver confers to glucokinase the property of being antagonistically regulated by fructose 6-phosphate and fructose 1-phosphate, 10.1111/j.1432-1033.1989.tb14538.x
  2. Van, Faseb, J., 8, 414 (1994)
  3. Detheux Michel, Vandekerckhove Joël, Van Schaftingen Emile, Cloning and sequencing of rat liver cDNAs encoding the regulatory protein of glucokinase, 10.1016/0014-5793(93)80089-d
  4. Cloning and sequencing of rat liver cDNAs encoding the regulatory protein of glucokinase : Michel Detheux, Joël Vandekerckhove, Emile Van SchaftingenFEBS Letters321 (1993) 111-115 (FEBS 12330), 10.1016/0014-5793(94)80437-0
  5. Studier F.William, Moffatt Barbara A., Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes, 10.1016/0022-2836(86)90385-2
  6. Marchuk Douglas, Drumm Mitchell, Saulino Ann, Collins Francis S., Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products, 10.1093/nar/19.5.1154
  7. Veiga-Da-Cunha Maria, Detheux Michel, Watelet Nathalie, Schaftingen Emile, Cloning and Expression of a Xenopus Liver cDNA Encoding a Fructose-Phosphate-Insensitive Regulatory Protein of Glucokinase, 10.1111/j.1432-1033.1994.00043.x
  8. VANDERCAMMEN Annick, SCHAFTINGEN Emile, The mechanism by which rat liver glucokinase is inhibited by the regulatory protein, 10.1111/j.1432-1033.1990.tb19147.x
  9. Towbin H., Staehelin T., Gordon J., Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications., 10.1073/pnas.76.9.4350
  10. DETHEUX Michel, VANDERCAMMEN Annick, SCHAFTINGEN Emile, Effectors of the regulatory protein acting on liver glucokinase: a kinetic investigation, 10.1111/j.1432-1033.1991.tb16218.x
  11. Hemmingsen Sean M., Woolford Carol, van der Vies Saskia M., Tilly Kit, Dennis David T., Georgopoulos Costa P., Hendrix Roger W., Ellis R. John, Homologous plant and bacterial proteins chaperone oligomeric protein assembly, 10.1038/333330a0
  12. Vandercammen A, Van Schaftingen E, Species and tissue distribution of the regulatory protein of glucokinase, 10.1042/bj2940551
Bibliographic reference Detheux, Michel ; Van Schaftingen, Emile. Heterologous expression of an active rat regulatory protein of glucokinase. In: FEBS Letters, Vol. 355, no. 1, p. 27-29 (1994)
Permanent URL http://hdl.handle.net/2078.1/11970