Lemarre, P
Robineau, S
Colson, A M
Netter, P
Three respiratory-deficient mutants of cytochrome oxidase subunit I in the yeast mitochondrion have been sequenced. They are located in, or near, transmembrane segment VI, the catalytic core of the enzyme. Respiratory-competent revertants have been selected and studied. The mutant V244M was found to revert at the same site in valine (wild-type), isoleucine or threonine. The revertants of the mutant G251R were of three types: glycine (wild-type), serine and threonine at position 251. A search for second-site mutations was carried out but none were found. Among 60 revertants tested, the mutant K265M was found to revert only to the wild-type allele.
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Bibliographic reference |
Lemarre, P ; Robineau, S ; Colson, A M ; Netter, P. Sequence analysis of three deficient mutants of cytochrome oxidase subunit I of Saccharomyces cerevisiae and their revertants.. In: Current genetics, Vol. 26, no. 5-6, p. 546-52 (1994) |
Permanent URL |
http://hdl.handle.net/2078.1/11949 |