Balzi, E.
Chen, W N
Capieaux, E.
McCusker, J H
Haber, J E
Goffeau, André
[UCL]
In Saccharomyces cerevisiae, the SCL-1 mutation is a dominant suppressor of the cycloheximide-resistant, temperature-sensitive (ts) lethal mutation, crl3 [McCusker and Haber, Genetics 119 (1988a) 303-315]. The wild-type scl1+ gene was isolated by screening subclones of the 35-kb region between TRP5 and LEU1 for restoration of the ts phenotype in an SCL1-1 crl3-2 strain. The scl1+ mRNA is about 900 nt long and encodes an open reading frame of 810 bp. The polypeptide deduced from scl1+ possesses a putative secretory signal peptide. The 5'-noncoding region may be under multiple controls, since it contains significant homology to the consensus sequences for the DNA-binding proteins, GCN4, GFI and, possibly, TUF. Gene disruption of scl1+ demonstrates that it is an essential gene.
Bibliographic reference |
Balzi, E. ; Chen, W N ; Capieaux, E. ; McCusker, J H ; Haber, J E ; et. al. The suppressor gene scl1+ of Saccharomyces cerevisiae is essential for growth.. In: Gene, Vol. 83, no. 2, p. 271-9 (1989) |
Permanent URL |
http://hdl.handle.net/2078.1/11635 |