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The action of brevin, an F-actin severing protein, on the mechanical properties and ATPase activity of skinned smooth muscle

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Gailly, Philippe [UCL] Lejeune, Thierry [UCL] Capony, J. P. Gillis, Jean-Marie [UCL]

Brevin is a protein which regulates the actin gel-sol transformation: it severs F-actin filaments into shorter ones. This action is Ca-dependent and is prevented by tropomyosin. We tested the effect of brevin on isometric contractions of skinned smooth muscle (taenia coli) and noted a dramatic loss of tension that possibly reflects some F-actin fragmentation. This effect is tentatively attributed to a partial loss of tropomyosin in the skinning procedure. We also studied the effect of brevin on unloaded shortenings of skinned preparations: thin bundles and enzymatically dissociated cells. We observed a marked increase of the velocity of shortening in the presence of brevin. This effect cannot be attributed to an increased ATPase activity as the latter is slightly reduced in the presence of brevin. We interpret this result as reflecting a decrease in internal resistance to movement, possibly by solation of an actin-filamin domain.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 1990
Journal information "Journal of Muscle Research and Cell Motility" - Vol. 11, no. 4, p. 293-301 (1990)
Peer reviewed yes
Publisher Springer Netherlands ((Netherlands) Dordrecht)
issn 0142-4319
e-issn 1573-2657
Publication status Publié
Affiliations UCL - MD/IEPR - Institut d'éducation physique et de réadaptation
UCL - MD/FSIO - Département de physiologie et pharmacologie
UCL - (SLuc) Service de neurologie
UCL - (SLuc) Service de médecine physique et de réadaptation motrice
MESH Subject Actins - metabolism ; Adenosine Triphosphatases - physiology ; Animals ; Calcium-Binding Proteins - physiology ; Gelsolin ; Glycerol ; Guinea Pigs ; Isometric Contraction - physiology ; Microfilament Proteins - physiology ; Muscle, Smooth - enzymology - physiology ; Time Factors
Links
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Bibliographic reference Gailly, Philippe ; Lejeune, Thierry ; Capony, J. P. ; Gillis, Jean-Marie. The action of brevin, an F-actin severing protein, on the mechanical properties and ATPase activity of skinned smooth muscle. In: Journal of Muscle Research and Cell Motility, Vol. 11, no. 4, p. 293-301 (1990)
Permanent URL http://hdl.handle.net/2078.1/11485