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Cloning and expression of a cDNA for the human homolog of mouse T cell and mast cell growth factor P40

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Renauld, Jean-Christophe [UCL] Goethals, Aline Houssiau, Frédéric [UCL] Van Roost, Emiel [UCL] Van Snick, Jacques [UCL]

A cDNA encoding the human homolog of mouse T-cell and mast cell growth factor P40 was derived from peripheral blood mononuclear cells (PBMC) stimulated with phytohemagglutinin and phorbol myristate acetate. Sequence analysis of the cDNA predicted a precursor protein of 144 amino acids including a signal peptide of 18 residues, a structure identical with that of mouse P40. The homology between the mouse and human proteins is 55% with a perfect conservation of the 10 cysteine residues present in the mature polypeptide. Expression of the cDNA for human P40 in a baculovirus vector yielded a protein capable of enhancing in vitro survival of human T cell lines.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 1990
Journal information "Cytokine" - Vol. 2, no. 1, p. 9-12 (1990)
Peer reviewed yes
Publisher Academic Press (London)
issn 1043-4666
e-issn 1096-0023
Publication status Publié
Affiliations UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique
UCL - MD/MINT - Département de médecine interne
UCL - (SLuc) Service de rhumatologie
MESH Subject Amino Acid Sequence ; Base Sequence ; Blotting, Northern ; Cloning, Molecular ; DNA - genetics ; Gene Expression ; Growth Substances - genetics ; Humans ; Interleukin-9 ; Interleukins - genetics ; Molecular Sequence Data ; RNA, Messenger - genetics
Keywords cDNA ; Growth factor ; T cells ; Human P40 cDNA
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Bibliographic reference Renauld, Jean-Christophe ; Goethals, Aline ; Houssiau, Frédéric ; Van Roost, Emiel ; Van Snick, Jacques. Cloning and expression of a cDNA for the human homolog of mouse T cell and mast cell growth factor P40. In: Cytokine, Vol. 2, no. 1, p. 9-12 (1990)
Permanent URL http://hdl.handle.net/2078.1/11464