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Molecular electron paramagnetic resonance imaging of melanin in melanomas : a proof-of-concept

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Vanea, Emilia Sabina [UCL] Charlier, Nicolas [UCL] Dewever, Julie [UCL] Dinguizli, Mustapha [UCL] Feron, Olivier [UCL] Baurain, Jean-François [UCL] Gallez, Bernard [UCL]

The incidence of malignant melanoma is increasing at an alarming rate. As the clinical outcome of the disease strongly depends on the localization of the lesion, early detection at the initial stages of development is critical. Here, we suggest spatial characterization of melanoma based on the presence of endogenous stable free radicals in melanin pigments. Taking into account the abundance of these naturally occurring free radicals in proliferating melanocytes and their localization pattern, we hypothesized that electron paramagnetic resonance (EPR) imaging could be a unique tool for mapping melanomas with high sensitivity and high resolution. The potential of EPR to image melanoma samples was demonstrated in vitro in animal and human samples. Using EPR systems operating at low frequency, we were also able to record in vivo EPR spectra and images from the melanin present in a subcutaneous melanoma implanted in a mouse. In addition to the proof-of-concept and the achievement of providing the first non-invasive image of an endogenous radical, this technology may represent a key advance in improving the diagnosis of suspected melanoma lesions. Copyright (c) 2008 John Wiley & Sons, Ltd.

Document type Article de périodique (Journal article) – Article de recherche – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 2008
Language Anglais
Journal information "NMR in Biomedicine" - Vol. 21, no. 3, p. 296-300 (2008)
Peer reviewed yes
Publisher JohnWiley & Sons Ltd. ((United Kingdom) Bognor Regis)
issn 0952-3480
e-issn 1099-1492
Publication status Publié
Affiliations UCL - MD/FARM - Ecole de pharmacie
UCL - MD/MINT - Département de médecine interne
UCL - (SLuc) Service de médecine nucléaire
UCL - (SLuc) Unité d'oncologie médicale
UCL - (SLuc) Centre du cancer
MESH Subject Mice, Inbred C57BL ; Mice ; Melanoma - diagnosis - pathology ; Humans ; Electron Spin Resonance Spectroscopy ; Animals
Links
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Bibliographic reference Vanea, Emilia Sabina ; Charlier, Nicolas ; Dewever, Julie ; Dinguizli, Mustapha ; Feron, Olivier ; et. al. Molecular electron paramagnetic resonance imaging of melanin in melanomas : a proof-of-concept. In: NMR in Biomedicine, Vol. 21, no. 3, p. 296-300 (2008)
Permanent URL http://hdl.handle.net/2078.1/11273